The secretion of ligninases by Phanerochaete chrysosporium was investigated with polyclonal antibodies, followed by immmunogold-silver staining and light microscopy. After growing fungal mycelium on nitrocellulose, extracellular ligninases were detected around old, plasmaless hyphae, but not at arthrospores, chlamydospores, or blastoconidia. Labeling of fungal hyphae on coverslip cultures was observe only when the cell wall was highly porous and translocation of cell material occurred. These experiments suggested that ther is a correlation between hyphal lysis and the release of extracellular ligninases from hyphae.

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