Basisiospore production by Lentinus lepideus in vitro
Evaluation of fungicides active against the lower fungi by spore based bioassays is very common. Possibilities of using similar assays to evaluate preservatives against brown rot and white rot fungi, especially for use in above ground constructions, are limited by the lack of methods for production of basidiospores. Tested media containing glucose 10-30 g/l and ammonium tartrate concentrations 2-20 g/l supported fairly good spore production. Media buffered with the organic buffer 2 (N-morpholino) ethane sulfonate (MES) with phosphate concentrations of 12.5-50 mM supported good spore production.