Many chemicals are used today to enhance the durability of wood and wood-derived products that are very important in our life. Such chemicals are copper, chromium, arsenate, zinc, etc. Though these preservatives are useful to protect wood from biodeterioration, but environmental toxicity is also related with them. The present study is to investigate the fungitoxic activities of Dalbergia sissoo leaves instead of chemicals. Acetone with five different concentrations was used as solvent to isolate the leaf extract of Dalbergia sissoo. The extractives were then used to see the inhibition activity over the soil fungi and other microbes. Experiment has showed that 3.0% concentration of acetone as solvent with leaf extract showed best result in inhibiting the attack of soil fungi and bacteria. For treating with aforesaid concentration of acetone, wood sample showed lowest loss of weight (22%). From this investigation, it can be assumed that biological preservative is a tool of protecting wood and thus environmental toxicity and biodiversity can be maintained.

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