In-house accelerated method for testing decay resistance of treated wood
Fungicidal compounds often change their effectiveness when they are incorporated into candidate formulations. For this reason fungistatic effectiveness needs to be re-evaluated as many times as the formula has been modified for better performance. To avoid multiple expenses, in-house fungistatic tests are essential. Our goal was to develop in-house usable, simple but reliable and reproductive procedure for testing decay resistance of the Sansin priming formulae without a need for special testing equipment.
Our preliminary trials have proved that commercially available, heat processed wood pellet fuel can be used as an excellent substrate for growing certain wood rotting fungi. In contact with water wood pellets quickly disintegrate and convert into sawdust, gaining up to three times increase in volume. When wood pellets are wetted with water containing hydrogen peroxide, the vapors of this antiseptic kill non-desirable air-borne contaminants and protect the substrate from further contamination for as long period as peroxide remains in the substrate at sufficient concentration. When peroxide protected substrate is spawned with well-organized fungal organisms (for example true decay/test fungi), they will rapidly colonize the substrate and develop a powerful mycelial network capable of decomposing most of the natural fiber based materials.
Treated and non-treated wood specimens were buried in spawned, hydrogen peroxide protected wood pellet fuel-based substrate and placed in transparent, perforated plastic containers to determine the effect of this procedure on rapid colonization and accelerated wood degradation. Parallel test was set up with specimens inserted in 150mm diameter dishes containing fungal cultures developed on the beer based agar medium. The results after 45 and 90 days of exposure to the brown rot causing fungus Gloeophyllum trabeum indicate that the weight loss in control blocks in containers was higher than in the dishes for 7 and 11 percent respectively.