Two methods were used to screen a number of potential anti-sapstain chemicals in the laboratory. In one method miniature boards of freshly felled Scots pine sapwood were dip treated with the candidate chemical, sprayed with a suspension of stainers or moulds and incubated for five weeks. In the other method antibiotic assay discs were treated with precise quantities of chemical, plated out on malt extract agar, inoculated with a spore suspension of one of four stainers and moulds and incubated for a few days. This was repeated for each of the four fungi. The results obtained from the two methods provided information on different aspects of the chemicals but the overall conclusions on efficacy were the same.

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