Gaseous treatment with allyl isothiocyanate (AIT) was tested for its effects on the growth of microorganisms on the wood substrate and the mortality of subterranean termites and powder-post beetles. Toxic limits of AIT were determined as concentrations in the air when an AIT-treated filter paper was placed in a sealed container with fungus-inoculated wood specimens. Those were <3.8 ppm for Aureobasidium pullulans, 7.5-15 ppm for Aspergillus niger, 30-59 ppm for Gliocladium virens, 59-118 ppm for Penicillium funiculosum and >118 ppm for Rhizopus stolonifer. LD 50 values were determined for insects. Twenty workers of Coptotermes formosanus or 10 adults of Lyctus brunneus were placed in an air-circulated glass bottle, and the mortality of the test insects was recorded after 24 hours. LD 50/24h were 10-13 ppm and approximately 80 ppm in the air for Coptotermes formosanus and Lyctus brunneus, respectively.

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