The isolation of DDAC tolerant fungi was carried out on lumber treated with a range of antisapstain chemicals containing DDAC as one of their active ingredients. The tolerant fungi were selected by using malt media spiked with DDAC (100 and 2500 ppm). Isolations were tentatively identified into a range of fungi commonly found associated with wood deterioration (i.e. Penicillium sp. and Trichoderma sp.). One particular group showed extreme tolerance to DDAC (growing at 2500 ppm) and were tentatively identified as fungi from Verticillium/Acremonium sp. DDAC was extracted from the above media and analysed using HPLC, in order to determine the fate of the DDAC once the fungi had colonised the plate.

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