Minimum inhibitory concentration of methylene bisthiocyanate towards selected wood inhabiting fungi in liquid culture and on radiata pine

IRG/WP 01-30261

T Singh, B Kreber, C Chittenden, R N Wakeling, A Stewart

The objective of the current study was to determine the minimum inhibitory concentration (MIC) of methylene bisthiocyanate (MBT) towards wood inhabiting fungi common on unseasoned radiata pine (Pinus radiata D. Don). The fungi used were Ophiostoma floccosum, Leptographium procerum, Sphaeropsis sapinea and Trichoderma viride. Fungi were individually inoculated into nutrient media spiked with different concentrations of MBT. After 12 days of incubation, biomass and microscopy were used to determine the MIC value for each fungus tested. For wood, radiata pine wafers were inoculated with individual fungi prior to dip-treatment with different concentrations of MBT. Fungal growth over the surface of wafers was assessed visually over five weeks and used an indicator to define a MIC value. Of all fungi tested, T. viride exhibited the greatest fungicidal tolerance in liquid media and on wood. For all fungi, spores and hyphal fragments were more sensitive to MBT than established hyphae, and considerably higher levels of MBT were required to kill growth of test fungi on wood than in liquid culture.


Conference: 01-05-20/25 Nara, Japan

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