Postia placenta gene expression during growth in furfurylated wood

IRG/WP 10-10734

G Alfredsen, C G Fossdal

Modified wood can provide protection against a range of wood deteriorating organisms. But we still lack information about why the modified wood is protected from microbial attack. Several hypotheses have been put forward for the mode of action against wood decaying fungi, including inhibition of action of specific enzymes, but they still need further testing. In this study gene expression of the brown rot fungus Postia placenta FPRL 280 has been monitored after 2, 4 and 8 weeks of colonization in furfurylated Scots pine (Pinus sylvestris L.) and in untreated control samples. Preliminary results are given. The main finding was that genes related to oxidative metabolic activity generally was higher in furfurylated wood compared to untreated Scots pine. Carbohydrate metabolism related expression varied. For one endo-glucanase and two β -glucosidases the expression was lower in furfurylated wood compared to untreated control, while for one glucoamylase and one glucan 1,3b glucosidase the expression was higher in furfurylated wood. The four cytochrome P450 tested, involved in breakdown of toxic compounds, gave inconsistent results between furfurylated and untreated control samples. Phenylalanine ammonia lyase and cytosolic oxaloacetase gave higher expression in control than in furfurylated samples.


Keywords: furfurylated wood, fungal colonization, gene expression, metabolic genes, Postia placenta, quantitative real time PCR

Conference: 10-05-09/13 Biarritz, France


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