An exploratory study was performed to probe into fungal/wood/fungicide interactions using Ophiostoma piceae, a common sapstaining fungus on unseasoned radiate pine. Treated (1% solution containing IPBC (0.07% w/w) and DDAC (0.60% w/w)) and untreated radiate pine wafers were inoculated with O. piceae and examined microscopically over a ten days incubation time. The results of the study showed that while prolific spore germination and mycelial growth of O. piceae occurred on untreated radiate pine, spore deformation and lysis was observed on treated radiate pine. Using Trypan blue, a vital stain, it was found that some swelled spores remained viable during incubation for ten days suggesting that IPBC/DDAC mode of action is to delay the process of spore germination.

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