Copper based preservatives have been extensively used in the field of wood preservation. However, several brown-rot fungi to be tolerant to copper and consequently, efficacy of copper based wood preservatives may not be sufficient. Copper tolerance is especially by some fungi that are closely aligned to or included in genus Antrodia. The highest copper tolerance was found at some strains of wood rotting fungus A. vaillantii. In this research, we investigated the variation of tolerance to copper among 14 isolates of Antrodia using different screening tests. Screening test on potato dextrose agar wasused as nutrient medium. The concentration of copper(II) sulfate in growth medium varied between 1.0x10-3 mol/l and 5.0x10-2 mol/l. There was almost no growth detected at all observed isolates when nutrient media contained the highest concentration of copper. Nevertheless, six strains were still able to grow on solid media containing 2.5x10-2 mol/l of copper. Further tests confirmed copper tolerance atselected isolates. Thus we can conclude that various isolates performed different copper tolerance.

Download document (142 kb)
free for the members of IRG. Available if purchased.

Purchase this document