Antagonistic abilities of a commercial biofungicide, Gliocladium virens (GL-21, Grace) were evaluated against three white-rot fungi, Trametes versicolor, Phlebia brevispora, Irpex lacteus, and three brown-rot fungi, Postia placenta, Neolentinus lepideus, Gloeophyllum trabeum. In dual culture of Gliocladium virens and wood decay fungi, Gliocladium virens rapidly overgrow the decay fungi and killed them. Pretreatment of Southern pine and maple blocks with Gliocladium virens prevented weight loss by all the decay fungi in soil-block tests. Gliocladium virens colonized blocks treated with propylene oxide to kill the antagonist were not decay resistant. Filter-sterilized filtrates from Gliocladium virens showed fungistatic effect against the decay fungi in agar medium. However, weight losses in wood blocks treated with filter-sterilized filtrates of Gliocladium virens were only slightly reduced when exposed to decay fungi in soil-block tests.

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