The objective of this study was to test the fractionated metabolites released by Coniophora puteana for their antagonistic activity against the sapstain fungi Ceratocystis coerulescens and Aureobasidum pullulans, and the molds Asperigillus niger and Penicillium spp. The acetone-soluble fraction obtained from the culture filtrate prepared from Coniophora puteana grown on 6% malt extract agar inhibited mycelial growth in a plate bioassay. The <5k-Da fraction separated from the acetone-soluble fraction also inhibited mycelial growth in the plate bioassay and prevented attack by Ceratocystis coerulescens in wood.

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