Results from agar block tests using Coniophora puteana demonstrate that the biological activity of alkyl dimethyl benzyl ammonium chloride is affected by the nature of the carrier solvent in which it is applied. In contrast to some earlier work, an alkyl ammonium compound in organic solvent formulation was identified that had significantly greater activity than its aqueous equivalent. A simple solution of alkyl dimethyl benzyl ammonium chloride in xylene did not exhibit increased activity relative to that of the same alkyl ammonium compound in aqueous solution. The reasons for the differences in performance cannot at present be fully explained.

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