Molecular studies on isolates of Serpula lacrymans
A Vigrow, D Button, J W Palfreyman, B King, B M Hegarty
The major protein species present in detergent extracts of 14 different Serpula lacrymans isolates have been compared, by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE), with a standard strain, viz. Serpula lacrymans FPRL 12C. Following silver staining of SDS gels the major protein species identified in 12 isolates were similar to those found in the standard strain. However differences were found when the final two isolates were compared with FPRL 12C, both isolates had extra molecular species not present in 12C and both were lacking some present in the standard strain. Comparison of the protein species identified in Serpula lacrymans isolates with those identified in extracts of other fungal organisms, viz. brown and white rot causing basidiomycetes and non-basidiomycetes indicated that the Serpula isolates were more similar to each other than to other organisms. Some molecular differences could be identified when individual isolates were cultured on different media, i.e. liquid culture or agar, only minor differences were seen when individual isolates were subcultured. These results indicate that whilst care must be taken to ensure as near identical conditions as possible for culture of organisms if their molecular species are to be compared by SDS-PAGE and silver staining, consistent results can be obtained using this technique. The technique may therefore offer a method of distinguishing between isolates, strains and species of wood decay basidiomycetes, and identifying new isolates.