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Applications of immunological methods to the analysis and detection of Lentinus lepideus Fr
1989 - IRG/WP 1422
Polyclonal antisera have been raised against the brown rot fungus Lentinus lepideus, a major cause of rot induced pole failure in the UK. Specificity studies have indicated that the antisera cross-react with a number of basidiomycetes but to much lesser extent with other fungi. The antigenicity of Lentinus lepideus and hence its molecular composition shows some alteration with culture age and change of substrate. Wood block decay experiments have indicated that dot-immunobinding assays based on the antisera can detect the presence of Lentinus lepideus in conditions of minimal weight loss, an initial field trial has indicated that cross-reacting antigens can be detected in artificially inoculated distribution pole stubs. These and other aspecta of the antigenicity and immunodetection of Lentinus lepideus are discussed in this paper.
H Glancy, A Bruce, D Button, J W Palfreyman, B King

Detection of brown-rot antigens in southern pine
1996 - IRG/WP 96-20090
Brown rot fungal antigens were detected by particle capture immunoassay (PCI) in southern pine 2 x 4's beyond visible or culturable hyphal growth. Further analysis of test samples revealed changes along the 2 x 4's that could be grouped into zones. Zone 1, the point of inoculation through 6 cm, showed low pH, measurable oxalic acid, high moisture, and high protein. Zone 2, through 16 cm, marked the end of visible hyphal growth, increased permeability, viable fungi isolated by culturing, and detection by microscopic examination. Zone 3, through 71 cm, revealed increased alkali solubility. Zone 4, through 84 cm, demonstrated positive PCI results (i.e. detection of fungal antigens). Further testing included mineral analysis which revealed iron accumulation. Movement of soluble fungal antigens beyond hyphal growth may account for the ability of the PCI to detect the presence of brown-rot fungi at zero percent wood weight loss.
C A Clausen