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Direct analysis from wood of the blue stain fungi Aureobasidium pullulans and Hormonema dematioides by denaturing gradient gel electrophoresis
2006 - IRG/WP 06-10595
Aureobasidium pullulans and Hormonema dematioides are the two organisms used in the EN 152 laboratory method for determining the effectiveness of preservatives against blue stain in service. The literature concerning the disfigurement of surface coatings and exposed timber in-service states that A. pullulans is the dominant blue stain fungus, due to its frequent isolation from stained material. In...
M J Ray, D J Dickinson


Mechanical and chemical properties of blue stained wood
2009 - IRG/WP 09-10686
Discoloration of wood is frequently caused by blue-staining fungi. In the previous researches there was general opinion that blue-stain fungi do not influence mechanical properties. On the other hand, there were some opposite results reported as well. In order to elucidate this issue, specimens made of Pinus sylvestris sapwood were exposed to two the most frequent and important blue stain fungi Au...
M Humar, V Vek, B Bučar


Degradation of Carbon-Based Preservatives by Black-stain Fungi
2010 - IRG/WP 10-30533
Some black-stain fungi are known to exhibit resistance to carbon-based preservatives. However, the cause of this resistance is unclear. The fungi may have mechanisms to tolerate these chemicals, or they may be able to degrade and detoxify them. Fungi capable of degrading carbon-based preservatives are of interest for possible bioremediation of carbon-based preservative-treated wood at the end of i...
R Stirling, P I Morris


Using an accelerated laboratory method to evaluate Aureobasidium pullulans colonization on painted surfaces and underlying wood substrate
2012 - IRG/WP 12-20480
Mold growth on painted surfaces can lead to premature paint film failure and is unsightly. Aureobasidium pullulans has been identified as a major cause of black stain on surfaces painted with acrylic or latex paints. In this study, an accelerated laboratory method developed by Zabel and Horner (1981) was used with modifications to the type of agar used and sample evaluation methods. A study was ...
C Schauwecker, L Burns, A Preston


Detection of Aureobasidium as the dominant fungus on coated wood
2013 - IRG/WP 13-10796
Wood is often covered with a coating in order to protect the surface. Unwanted dark mold growth on paint covered surfaces in external conditions is a familiar phenomenon. Simple microbial techniques have commonly been used to determine the dominant fungal species present in the discoloured area, for example morphological analysis of microscopic preparations of fungal pieces sticking to an adhesive...
E J Van Nieuwenhuijzen, M F Sailer, R A Samson, O C G Adan


The role of hydrophobins in surface growth by the Blue Stain fungi Aureobasidium pullulans
2015 - IRG/WP 15-10837
Hydrophobins are small proteins found exclusively in filamentous fungi. These proteins are able to assemble spontaneously into amphiphilic monolayers at hydrophobic–hydrophilic interfaces. Hydrophobins have a diverse role, e.g. allowing the fungi to break through interfaces during aerial hyphae formation, sporulation, fruit body production and cell penetration. In this ongoing study, we are look...
J Stenbæk, L Riber, J Blæsbjerg Nielsen, C Møller Hansen, B Jensen


Effects of UV Radiation on Melanization and Growth of Fungi Isolated from Weathered Wood Surfaces
2015 - IRG/WP 15-10842
This research tests the hypothesis that fungi isolated from weathered wood will respond to elevated levels of UV radiation by increasing their melanin production. To test this hypothesis, we measured the melanin production and mycelial color of Aureobasidium pullulans and Cladosporium cladosporioides, before and after the fungi were grown under artificial UV, visible light and complete darkness. T...
V A Hernández, P D Evans


Thermal stability of a wood protective biofinish and the influence of flame retardants on Aureobasidium cells
2017 - IRG/WP 17-30716
In general there is an increasing need for sustainable resources, including sustainable building materials. Wood is one of these sustainable resources and is more used as an outdoor building material. Since unprotected wood will usually be degraded during outdoor exposure, many different protection systems are available on the market. Protection systems containing biocides however might create obs...
S Rensink, E A M Klein Rot, M F Sailer


Effects of wood protecting biofinish and linseed oil on fire behaviour and leachability of the fire retardant
2018 - IRG/WP 18-30728
A wood protecting biofinish is based on a protective and decorative fungal-based coating and a linseed oil impregnation (called Xyhlo biofinish). This biofinish enables the long-term use of wood in outdoor applications without using toxic chemicals. The fire resistant properties of materials used in buildings are very important. Since Xyhlo biofinish is relatively new, only little information is ...
S Rensink, E J van Niewenhuijzen, M F Sailer


Biofinish: A Functional Wood Surface Treatment based on Aureobasidium
2020 - IRG/WP 20-40903
Xyhlo Biofinish is a natural and environmentally friendly wood protection concept based on linseed oil impregnation and a fungal-based coating. It emerged in the late 1990s and has been developed over the past 15 years into an industrially applicable process in the Netherlands. The combination of linseed oil impregnation and a surface treatment with the living fungus Aureobasidium extends the serv...
S Rensink, M Sailer, S Roukens, J Gerber, H van der Mel, K Potgieter, J Spit, R Bulthuis, C Struck, M Bennink


Comparison of culturable fungi found on two types of modified wood cladding
2023 - IRG/WP 23-11025
Modified woods are rapidly gaining popularity in the United States and are appearing on more public buildings. Because these are biocide-free products, they can support the growth of non-wood degrading fungi on their surfaces, many of which produce dark pigments and deface the cladding. Even growth of dark pigmented fungi on modified woods yields an even grey-black color, giving the materials a we...
G Presley, J Cappellazzi, B Held, R Blanchette


Time-lapse evaluation of Aureobasidium pullulans growth on selected lignin derivatives and chitosan
2024 - IRG/WP 24-11032
Wood, often referred to as the “building material of the 21st century”, has gained recognition as an attractive alternative to several traditional building solutions. To enlarge the application of wood, several properties including biotic and abiotic degradation resistance need to be improved. Consequently, new solutions are available on the market that ensure expected properties and functiona...
Gubensek, F Poohphajai, A Tosic, K Butina Ogorelek, W Pajerski, A Cernosa, V Hribljan, A Sandak


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