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Effects of the anaerobic wood decay bacterium Clostridium xylanolyticum on unbleached Pinus and Eucalyptus pulp
1991 - IRG/WP 1506
Clostridium xylanolyticum has been shown to produce extracellular enzymes capable of degrading wood. The present work was conducted to quantify growth on various lignocellulosic substrates and degradation of pulped wood fibre. In the latter tests Clostridium xylanolyticum was incubated at 35°C under anaerobic conditions in a medium containing 0.2% (w/v) peptone and 4% (w/v) unbleached Pinus and E...
G D Shelver, U Matai, W Van Wyk, A A W Baecker
Immunolocalization of xylanase produced by Clostridium xylanolyticum Rogers and Baecker during anaerobic decay of Pinus patula
1991 - IRG/WP 1505
The xylanolytic fraction from a 72h culture of Clostridium xylanolyticum ROGERS and BAECKER was separated using non-denaturing PAGE and then used to raise polyvalent antisera in rabbits. Antibodies were purified using an affinity gel column and coniugated with colloidal gold. Pinus patula was exposed to axenic cultures of Clostridium xylanolyticum for 3 weeks, fixed, embedded and converted to ultr...
A A W Baecker, G M Rogers