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Comparative response of Reticulitermes flavipes and Coptotermes formosanus to borate soil treatments
1991 - IRG/WP 1486
Eastern (Reticulitermes flavipes [Kollarl]) and Formosan (Coptotermes formosanus Shiraki) subterranean termite workers (Isoptera: Rhinotermitidae) were exposed to borate-treated sand in an indirect exposure tunneling assay in the laboratory. In the ten day assay period, both termite species readily penetrated sand containing 5000, 10000, or 15000 ppm (wt. of compound / wt. of sand) disodium octaborate tetrahydrate (Tim-BorÒ) or zinc borate (Firebrake ZB-FineÒ). With Reticulitermes flavipes, significant mortality (85-93%) resulted from workers tunneling through sand treated with 5000 ppm disodium octaborate tetrahydrate (higher concentrations were also effective), or 15000 ppm zinc borate. Responses of Coptotermes formosanus workers were lesser and more variable, with only concentrations of 10000 and 15000 ppm zinc borate resulting in mortality 70-89%) significantly different from that in the control groups. These results suggest that differences between these two species in tunneling behavior may reduce exposure of Coptotermes formosanus to the borate-treated sand.
J K Grace

International collaborative laboratory comparison of two wood preservatives against subterranean termites: Third update and first report
1996 - IRG/WP 96-10174
At the 24th annual meeting of IRG in Orlando, USA, in May 1993 an international subterranean termite laboratory bioassay to compare the various preferred termite protocols used by IRG termitologists was initiated. The author was nominated to co-ordinate this comparative laboratory evaluation of two wood preservatives, copper-chrome-arsenic (CCA) and copper naphthenate (Cu-Na) against the subterranean termites used as test termites in Australia, France, Japan, Thailand, United Kingdom and the Unites States of America. Solutions of these two wood preservatives were prepared and impregnated into Pinus radiata wood blocks to obtain loading of 0.0, 0.5, 1.0, 2.0 and 4.0 kg/m³ respectively. All preservative treatments were carried out at the Division of Forestry and Forest Products in Melbourne. The treated specimens were dispatched to the participating researchers who subjected these specimens to attack by their test termite species, and have now returned the specimens to Melbourne. This paper reports the amount of wood consumed and the mean mass loss (%) on both treated and untreated wood blocks by the termites in the various laboratory bioassays.
J R J French

Detection of feeding behaviour of termites using AE monitoring
1991 - IRG/WP 1514
Using acoustic emission (AE) monitoring, the feeding activity of the termite inhabiting a wood specimen was investigated. The amplitude and the rate of AE from the specimen of Coptotermes formosanus Shiraki was larger than that of Reticulitermes speratus Kolbe. The AE event rate was higher in the specimen with soldiers than without soldiers. The AE event rate decreased according to the resistance of wood specimens against termite attack.
Y Imamura, M Tokoro, M Owada, Y Fujii, M Noguchi

Trials on the field control of the Formosan subterranean termite with Amdro® bait
1982 - IRG/WP 1163
Amdro® - treated paper towels were introduced into two field colonies of the Formosan subterranean termite in Hawaii. At the concentration of 180 ppm, the toxicant bait was ineffective one month after the introduction. At higher concentrations (> 6,400 ppm), the baits were eaten initially; however, one week after introduction, termites avoided or covered the baits. The 15,000 ppm baits supressed the activity of one colony but did not affect the other.
N-Y Su, M Tamashiro, J R Yates III

Laboratory evaluation of chemicals as termiticides
1986 - IRG/WP 1293
Laboratory procedures are described for screening chemicals against subterranean termites. Fast-acting compounds with persistent termiticidal activity are identified in tests using a soil substrate, and slower-acting bait toxicants are evaluated in a series of tests using cellulose substrates.
S C Jones

Biological control of the Formosan subterranean termite Coptotermes formosanus Shiraki
1971 - IRG/WP 100
M Tamashiro, J K Fujii, P Lai, T E Richardson

Fungicidal and termiticidal effectiveness of alkylammonium compounds
1983 - IRG/WP 3232
This paper is related to effectiveness of several AAC's against wood decay fungi and termites by Japanese standardized test methods.
K Tsunoda, K Nishimoto

Termite resistance of pine wood treated with chromated copper arsenates
1997 - IRG/WP 97-30128
Two four-week, no-choice laboratory tests were performed with CCA-treated southern yellow pine and radiata pine against Formosan subterranean termites, Coptotermes formosanus. CCA retentions as low as 0.05 kg/m3 (0.03 pcf) provided protection from all but light termite attack (rating of 9 on a 10-point visual scale). Similar and consistent light attack on wafers containing retentions as high as 6.4 kg/m3 (0.4 pcf), coupled with complete termite mortality, demonstrates that the mode of action of CCA treatments relies upon toxicity rather than having any repellent effects against termites.
J K Grace

Termite trail-following substances in Houttuynia cordata
2001 - IRG/WP 01-10409
Termite trail-following active compounds in the plant, Houttuynia cordata Thunb., were studied by a combination of chemical analyses and bioassay using Coptotermes formosanus Shiraki as a test termite. We have found that termites were attracted to methanol extracts from H. cordata. The n-hexane(Hex) extracts, that showed a trail-following activity, were fractionated by a silica-gel column chromatography with Hex/ethyl acetate(EtOAc) successively increasing the polarity. The obtained 5%, 10%, 15% EtOAc/Hex fractions were found to be active. GC-MS analysis of the 5% EtOAc/Hex fraction showed a peak corresponding to 2-undecanone, which revealed weak activity.
W Ohmura, K Yamamoto, M Saegusa, T Ohira, A Kato

Response of the Formosan Subterranean Termite (Coptotermes formosanus) to Cellulose Insulation Treated with Boric Acid in Choice and No-Choice Tests.
2004 - IRG/WP 04-10532
The tunneling ability of the Formosan subterranean termite Coptotermes formosanus Shiraki through a cellulose insulation material containing11.1% boric acid was tested in choice and no-choice bioassays. We examined tunneling behavior and mortality of termites exposed to treated and untreated insulation material in miniature simulated wall voids. In a choice test termites tunneled through untreated insulation in all but one of the replicates used. Termites were unable to fully penetrate any of the replicates containing treated insulation and experienced a significantly higher mortality (78.4 ± 18.4%) than termites exposed to untreated insulation (11.6 ± 5.6%, F = 60.4, df = 1, P < 0.0001). In a no- choice test termites fully penetrated all replicates containing untreated insulation and experienced 37.1 ± 37.2% mortality. Termites exposed to treated insulation in this test experienced a significantly higher mortality of 100.0% (F = 14.3, df = 1, P < 0.005), and did not fully penetrate the treated insulation.
M E Mankowski, J K Grace

Microcapsule formulation of fenitrothion as a soil termiticide
1991 - IRG/WP 1478
The efficacy and the mode of action of a microcapsule formulation of fenitrothion against Coptotermes formosanus Shiraki were investigated. The physicochemical property that this formulation does not allow the active ingredient to diffuse through the capsule wall contributed to a long lasting efficacy and safety for the men spraying. The residual effect of the fenitrothion microcapsule in soil was revealed as well as that of chlordane in the laboratory test. It was clarified that the transmission of poisoning through the worker&apos;s self- and mutual grooming behavior contributed to the efficacy of this formulation. And it was suggested that the transmission of poisoning of fenitrothion through mutual grooming led to the collapse of a colony.
H Teshima, T Itoh, Y Abe

Fipronil - le nouvel insecticide de Rhone Poulenc pour la preservation du bois
1995 - IRG/WP 95-30078
After the 3rd International Symposium in Cannes &apos;95. More trial results and practical information on FIPRONIL, this new insecticide from Rhone Poulenc for Wood Preservation and PCO.
M G E J Van Maanen

Studies on the infesting behaviour of the Formosan termite, Coptotermes formosanus Shiraki and its physical control
1983 - IRG/WP 1174
An outline of termite damage to buildings in Japanese National Railways, wood-infesting behavior, attacked traces in PVC-sheathed cables by termites, detection method of termites and the physical control method of the Formosan termite are given in the present paper.
K Yamano

Testing of termiticides in soil by a new laboratory method with regard to Phoxim for replacement of chlorinated hydrocarbons
1986 - IRG/WP 1292
In comparison to chlorinated hydrocarbons insecticides of the compound classes organophosphates, carbamates and pyrethroids were tested according to an earlier described soil-test in the laboratory. The following termite-species were used: Heterotermes indicola, Reticulitermes santonensis and in addition Reticulitermes flavipes, Reticulitermes lucifugus, Reticulitermes speratus and Coptotermes formosanus (Rhinotermitidae). The method gives reliable results. Criteria for evaluation are mortality and penetration of the termites into the treated soil. According to this test organophosphates, specially phoxim, have a good potential to replace chlorinated hydrocarbons.
R Pospischil

Sequential exposure of borate treated Douglas-fir to multiple Formosan subterranean termite colonies in a 40-week field test
1993 - IRG/WP 93-10006
Douglas-fir boards (ca. 74.5 g) pressure-treated with disodium octaborate tetrahydrate (DOT) retentions of 0 (controls), 0.88, 1.23, 1.60, or 2.10% (weight/weight) DOT were sequentially exposed to four active field colonies of Formosan subterranean termites, Coptotermes formosanus Shiraki (Isoptera: Rhinotermitidae), in an above-ground field test. Samples were placed in contact with each colony for 10 weeks, with oven-dry weight losses determined between exposures, for a total termi exposure period of 40 weeks. Feeding activity differed among termite colonies, with the control wood samples having mean weight losses of 1.3-15.1% of their initial weight during each individual 10-week termite exposure. The two lower borate retentions (0.88 and 1.23% DOT) were virtually equal efficacy, with mean wood weight losses during each individual 10-week exposure ranging from 1.2-4.6%. Feeding was negligible at the two higher borate retentions, with mean wood weight losses from termite feeding during each 10-week period ranging from 0.7-1.3% with 1.60% DOT, and 0.3-0.9% with 2.10% DOT. Total cumulative wood weight losses over the 40 week exposure were: 10.2% (0.88% DOT), 8.7% (1.23% DOT), 3.6% (1.60% DOT), and 2.4% (2.10% DOT).
J K Grace, R T Yamamoto

Results on termite resistance of building materials against Coptotermes formosanus by choice test
1998 - IRG/WP 98-10275
Various building materials, included wood species, wooden board materials, thermal insulation materials and fire-protection materials, were tested for grading of termite resistance against Coptotermes formosanus. The dimension of most specimens were 2x2x2cm3. Ten repeats were prepared. The specimens were put between Akamatsu sapwood control specimens on a laboratory cultured mound colony of termite, Coptotermes formosanus. After 1 month of attack to termite, the specimens were removed from the mound colony and cleaned up. Then these final mass were weighed. The grading of termite resistance was initially estimated by mass loss of specimens. This grading was corrected by visual observation. Japanese 3 domestic species, cypress pine, Alaska- ceder, kapur and mahogany were indicated rather high termite resistance. In the case of Siberian red pine and Gmelina, the valued of termite resistance were shown variable. Tropical species plywood, inorganic board and radiata pine MDF, were shown rather high termite resistance. Other board materials were shown rather less termite resistance. Most of commercial soft wood plywood and OSB were very sensible against termite. Most common thermal insulation materials in Japan were estimated very sensitive against termite. In the case of fire protection materials, expanded concrete was rather good against termite but plaster board was very sensible against termite.
K Suzuki, K Hagio

Differences in feeding activity among colonies of Formosan subterranean termite Coptotermes formosanus Shiraki
1983 - IRG/WP 1202
Feeding activities of 7 colonies of the Formosan subterranean termite, Coptotermes formosanus Shiraki, were examined. Wood-consumption rates among colonies differed significantly, ranging from 23.80-78.48 mg/g/day. This large intraspecific variation raised a question of whether differences in feeding activity reported for other termite species were due to interspecific differences. When rates were expressed as mg wood consumed by one g termite per day (mg/g/day), termites of larger body weight appeared to consume less wood. This negative correlation, however, was not significant when rates were expressed as mg wood consumed by an individual per day (mg/worker/day).
N-Y Su, J P La Fage

Treatment of Douglas fir heartwood with disodium octaborate tetrahydrate (Tim-BorÒ ) to prevent attack by the Formosan subterranean termite
1991 - IRG/WP 1487
Toxicity of disodium octaborate tetrahydrate (Tim-BorÒ) to Coptotermes formosanus Shiraki (Isoptera: Rhinotermitidae), and termite feeding on treated Douglas-fir heartwood were evaluated in laboratory and field tests. Feeding on filter papers impregnated with Tim-BorÒ solutions reduced but did not eliminate termite gut protozoan populations. In a forced-feeding laboratory assay, Douglas-fir heartwood treated to Tim-BorÒ retentions ³0.35% BAE drastically reduced termite feeding and resulted in 100% termite mortality within three weeks. Gradual and significant mortality (49%) after four weeks of feeding at 0.16% BAE suggests that this or lesser concentrations may be useful in baits for remedial termite control. After 162 days of field exposure to an active Coptotermes formosanus colony in an accelerated field test, moderate feeding was noted at 0.65% BAE (13.6% weight loss) and 0.73% BAE (16.9% wt. loss), and only slight damage (2.5% wt. loss) at the highest retention field tested of 1.02% BAE. These results indicate that Tim-BorÒ provides protection from Formosan termite attack, but that some cosmetic damage occurs even at high retentions. This cosmetic damage is unlikely to create a structural hazard, but additional field evaluations are needed to determine the treatment requirements for timbers visible to the consumer.
M Tamashiro, R T Yamamoto, J K Grace

Termiticidal effectiveness of synthetic pyrethroids, carbaryl and chlordane in wood-block test
1985 - IRG/WP 3356
The results of some wood-block tests with allethrin, permethrin, fenvalerate, carbaryl and chlordane against Coptotermes formosanus are tabled and compared against some organophosphates, chlorpyrifos, fenitrothion, phoxim, tetrachlorvinphos and acephate. The treatment of the wood blocks was exactly as described in Document No: IRG/WP/3330. Among the pyrethroids tested, permethrin was the most effective. However this was not so effective as the organophosphates such as chlorpyrifos, phoxim and tetrachlorvinphos.
K Tsunoda

Methane emission by termites, Coptotermes formosanus Shiraki
1995 - IRG/WP 95-10099
Association of methanogenic bacteria only with the smallest-sized symbiotic protozoa Spirotrichonympha leidyi Koidzumi was evidenced by epifluorescence microscopic observations. Workers, which were collected from a laboratory colony and placed in a test container with water supply emitted methane at a relatively constant rate with a peak of 0.76 nmol/termite/hr within the first 72 hrs after the initiation of measurement. Soldiers, as expected, produced less methane with a maximum rate of 0.019 nmol/termite/hr. Although methane formation is considered important to termites in order to keep physiological balance, that undesirably contributes to global warming.
K Tsunoda, W Ohmura, M Tokoro, T Yoshimura

Laboratory evaluation of phoxim in plywood
2001 - IRG/WP 01-30264
Two types of phoxim treated plywood bonded by phenolic resin glue and urea resin glue were challenged with termites of Coptotermes formosanus Shiraki in laboratory. It was found that the sawdust of phoxim treated plywood bonded by phenolic resin glue was not active or did not kill termites of Coptotermes formosanus Shiraki within the test period. Moreover, the phoxim treated plywood bonded by phenolic resin glue did not prevent damage from termite attack. At the end of the test period, the plywood mass loss rate was about 21.81%. The gas chromatography was used to estimate the degradation rate of phoxim in phenolic and urea resin glue and the recovery rate of phoxim in plywood bonded by phenolic and urea resin glue. The results of analysis indicated that 7.48% of phoxim in phenolic resin glue was recovered after aging 8 hrs, and only traces of phoxim in plywood which was treated with phoxim and bonded by phenolic resin glue were found.
Zhong Junhong, Lin Liling, Huang Hong

Tunneling ability of subterranean termites through termiticide-treated soil
1988 - IRG/WP 1375
A test apparatus was designed to facilitate accurate measurement of termite penetration into termiticide-treated soil; three termiticides at 500 ppm were evaluated. Coptotermes formosanus Shiraki workers tunneled significantly farther into chlordane-treated soil than did Reticulitermes flavipes (Kollar). Both species only slightly penetrated chlorpyrifos-treated soil. Neither species penetrated soil treated with permethrin. After 7 days, termite survival was highest in permethrin tests, presumably because termites avoided contact with treated soil. Survival was lowest in chlorpyrifos tests, which may be attributed to contact toxicity as well as vapor toxicity.
S C Jones

Evaluation of termiticides in field trials
1990 - IRG/WP 3633
Termiticide-treated posts and stakes have been tested at the field test site in Kagoshima, Southern Kyushu, Japan. Various commercial and alternative termiticides have been evaluated annually as TAI (termite attack index), calculated by the equation: TAI = R x P, where R is the mean of attack rating of 0 (sound), 10 (sign of tasting), 30 (slight attack), 50 (moderate attack), 100 (severe attack), and P is the ratio of attacked posts (stakes) to total posts (stakes) tested, expressed as 0.0-1.0 . CCA-impregnated Japanese cedar (Cryptomeria japonica) posts have well performed keeping 0 TAI over nine years. AAC-treated pine (Pinus radiata) stakes with 9.3 kg/m³ DDAC (didecyldimethyl ammonium chloride) or 9.4 kg/m³ BTAC (methylalkylbenzyltrimethyl ammonium chloride) have been free from decay, but they yielded 34 or 42 TAI after eight years. Improvement of termite resistance was revealed by addition of cupric chloride or cuprammonium chloride. Brushing treatment of pine (Pinus densiflora) stakes, with 200 g/m chlordane (2.0%), chlorpyrifos (1.0%) or tetrachlorvinfos (1.0%) solutions, did not provide the perfect control for five years, yielding 30, 42 and 48 TAI, respectively.
M Takahashi, Y Imamura, K Tsunoda, A Adachi, K Nishimoto

Methods for testing fumigant efficacies against termites
1986 - IRG/WP 1297
Methodologies for testing fumigants against termites are reviewed and factors needed to be taken under consideration for standardization listed. Toxicity should be defined by both direct exposure to the gas and under more practical "barrier" conditions which include test enclosures simulating abiotic surroundings of the termites, i.e. wood, nest material, etc. To observe latent effects, mortality should be recorded periodically after exposure until rates decrease to control levels. Fumigant efficacy should be reported as a function of concentration and exposure time, termed lethal accumulated dose (LAD).
N-Y Su, R H Scheffrahn

Effects of methoprene on Coptotermes formosanus (Isoptera: Rhinotermitidae)
1987 - IRG/WP 1322
Methoprene affected differentiation and survival of Coptotermes formosanus Shiraki in laboratory studies. At 5, 9, 13, and 17 weeks, superfluous intercastes and presoldiers were produced when termites were allowed to feed on concentrations of 1,000 and 2,000 ppm methoprene in wood blocks. Colony numbers were significantly reduced after 13 and 17 weeks of exposure to the insect growth regulator. Termites are significantly less of the treated blocks than of the controls and sometimes physically sealed off the treated blocks, which suggests that some concentrations of methoprene may have slight antifeedant properties. Colonies varied in their responses to this chemical.
S C Jones

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