Your search resulted in 4 documents.
Immunogold localisation of catalytic domain of endocellulase in the cellulosic substrates
1997 - IRG/WP 97-10205
The reaction of catalytic domain of endocellulase on the cellulosic substrates was visualised by using immunogold labelling procedures to obtain a better understanding of the mechanism of enzymatic cellulose hydrolysis. Polyclonal antibody was produced against the catalytic domain of endo-l, 4-ß-glucanase (EGI) from a ruminal anaerobic bacterium Ruminococcus albus F-40. Immuno-TEM works showed th...
Yoon Soo Kim, Seung-Gon Wi, Kyu Ho Myung, K Ohmiya, S Karita
Immuno-electron microscopic localization of extracellular metabolites in spruce wood decayed by brown-rot fungus Postia placenta
1990 - IRG/WP 1441
Degradation by Postia placenta in spruce and birch wood was shown to occur not only in the wood cell wall but also in the middle lamellae region. Middle lamellae was often found to be degraded along the centerline so that cells could separate along this line. Extracellular membrane structures were found surrounding the hyphae and this matrix labelled positively with antisera produced to Postia pla...
Y S Kim, B Goodell, J Jellison
Immuno-scanning electron microscopic localization of extracellular polysaccharidases within the fibrillar sheath of the brown rot fungus Postia placenta
1991 - IRG/WP 1497
Extracellular polysaccharidases of the brown-rot fungus Postia placenta were localized using colloidal gold labeled monoclonal antibodies to the B-1,4-xylanase (32-36kDa) fraction of Postia placenta. Postia placenta was grown from agar onto glass coverslips, immunolabeled with or without prior fixation, and examined by SEM. Enzymes were localized on the hyphal surface and on the clumped fibrillar ...
F Green III, C A Clausen, M J Larsen, T L Highley
Development of a new characterization method to analyse Cypermethrin penetration in wood material by immunolabelling
2015 - IRG/WP 15-30674
The preservative efficacy of organic biocides is strongly related to their capacity of penetration and retention within wood tissues. The specific detection of the pyrethroid insecticide cypermethrin is currently obtained after extraction followed by chemical analysis by chromatography techniques. However visualizing the insecticide molecule within the wood structure requires specific probes toget...
S Tapin-Lingua, K Ruel, J-P Joseleau, D Messaoudi, O Fahy, M Jequel, M Petit-Conil