Your search resulted in 6 documents.
rDNA-ITS sequence of Serpula lacrymans and other important indoor rot fungi and taxon-specific priming PCR for their detection
1999 - IRG/WP 99-10298
Taxon-specific priming polymerase chain reaction (TSPP) is a powerful molecular tool for fungal diagnosis. For its application to indoor rot fungi, the internal transcribed spacer (ITS) of the ribosomal DNA (rDNA) of the main fungal species causing wood rot in European buildings was amplified with the polymerase chain reaction (PCR). The ITS region was sequenced. The complete sequences are present...
O Schmidt, U Moreth
Detection of the dry rot fungus Serpula lacrymans by amplified ribosomal DNA restriction analysis
1998 - IRG/WP 98-10245
Isolates of the dry rot fungus Serpula lacrymans and the wild merulius S. himantioides were investigated by amplified ribosomal DNA restriction analyses (ARDRA) of the internal transcribed spacers (ITS). The technique uses the polymerase chain reaction (PCR) to amplify the variable region of the ITS between the conserved 18S and 28S genes of the nuclear ribosomal DNA (rDNA).
The ITS region of all isolates of Serpula lacrymans and S. himantioides was successfully amplified. The length of the amplified product was about 630 bp. Subsequent double digest of the ITS with the restriction endonucleases Haelli and Taql separated the closely related fungi by species-specific fragments. Thus, ARDRA-ITS proved to be suited for the detection of the dry rot fungus.
O Schmidt, U Moreth
Molecular characterization and biodiversity of wood-decaying fungi in French Guiana
2014 - IRG/WP 14-10825
Fungi from tropical regions are currently under-represented in the classification system. Indeed, difficult access to tropical forests and irregular occurrence carpophores make it complicated to study fungus species in such environments, unlike in European zones where fungal diversity and taxonomy are better known. The purpose of this work was to enhance classification by integrating new data that...
A Zaremski, L Gastonguay, C Zaremski, J Beauchene
Biodiversity of wood-decaying fungi in French Guiana sequences of the small subunit (SSU) of ribosomal (r) DNA and the two primer pairs SR6/SR10R and SR7/SR1R
2019 - IRG/WP 19-10936
Fungi from tropical regions are currently under-represented in the classification system. Indeed, difficult access to tropical forests makes it complicated to study fungus species in such environments, unlike in European zones where fungal diversity and taxonomy are much better known. The purposes of this work were: a) to enhance classification by integrating new data that would bring out the impo...
C Zaremski, A Ducousso-Detrez, N Amusant, A Zaremski
Role of microbiota in wood degradation by Reticulitermes grassei and Bursaphelenchus xylophilus
2022 - IRG/WP 22-20684
Xylophagous organisms can cause damage both in forests and in felled wood. Bursaphelenchus xylophilus, which causes "Sudden Pine Wilting" in coniferous forest masses, and is currently considered a quarantine organism in the European Union. On the other hand, structural and carpentry wood is affected by subterranean termites (Reticulitermes spp.), that cause serious damage, both in buildings and fu...
L Robertson, S Rames, M Uriel, J M González, F Llinares, S M Santos, M T Troya
Isolation of the microbiota from wood decay insects and some of their enzymatic activities
2023 - IRG/WP 23-11014
Wood decay organisms have the ability to degrade wood components (cellulose, hemicellulose and lignin) by enzymatic capacities, but these enzymes can belong to the proper organism, or to the associated microbiota. The identification of the microorganisms, as well as their role in the digestion of many substrates not always is very well known.
The wood decay insects produce big damages in wood str...
M T Troya, F Llinares, J Arranz, S Izpura-Luis, S M Santos, L Robertson