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Detection of the dry rot fungus Serpula lacrymans by amplified ribosomal DNA restriction analysis
1998 - IRG/WP 98-10245
Isolates of the dry rot fungus Serpula lacrymans and the wild merulius S. himantioides were investigated by amplified ribosomal DNA restriction analyses (ARDRA) of the internal transcribed spacers (ITS). The technique uses the polymerase chain reaction (PCR) to amplify the variable region of the ITS between the conserved 18S and 28S genes of the nuclear ribosomal DNA (rDNA).
The ITS region of all isolates of Serpula lacrymans and S. himantioides was successfully amplified. The length of the amplified product was about 630 bp. Subsequent double digest of the ITS with the restriction endonucleases Haelli and Taql separated the closely related fungi by species-specific fragments. Thus, ARDRA-ITS proved to be suited for the detection of the dry rot fungus.
O Schmidt, U Moreth
Detection and Assessment Healthy Situation of Poulus Euphratica Oliv. with Stress Wave
2009 - IRG/WP 09-20415
The defects of tree trunk of Poulus euphratica Oliv were inspected by stress wave method and diagnosed with transmission time and velocity. The reference values of transmission time of unit length and velocity from stress wave were used to assess the healthy situation of tree trunk. The goal is provide fast inspecting technology and assessment method for the historic trees in China. The results sh...
Shanqing Liang, Nana Hu, Lanying Lin, Feng Fu