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Fungicidal Effect of Tobacco Stalks (Nicotiana tabacum L.) Against Brown Rot Fungi “Coniophora puteana”
2016 - IRG/WP 16-10865
In this study it was investigated that the fungicidal effect of tobacco plant (Nicotiana tabacum L.), which is grown in many countries of the world, against brown rot fungi “Coniophora puteana”. The protective effect of this plant on Scotch pine (Pinus sylvestris L.) wood was considered by means of fungi decay test (EN 113). The wood samples have been treated by five different extracts; at 3%, 5%, 10%, 15% and 20 % concentrations. As a result of the study it was found that the retention values increased with increasing the concentration of the Tobacco plant, in all variations. Weight losses of all test samples were lower than untreated samples. The best performance was obtained from the variation in 15 % concentration. However, tabocca plant has been found not efficient enough to protect the wood against fungi decay (Coniophora puteana) according to the European norms.
Ü C Yıldız, S Yıldız, A Yılmaz, S Durmaz

Effect of acetylation on decay resistance of wood against brown-rot, white-rot and soft-rot fungi
1989 - IRG/WP 3540
Effect of acetylation on decay resistance of wood was investigated using wood blocks of Cryptomeria japonica, Pinus densiflora, Albizia falcata and Fagus crenata. Blocks were treated with uncatalyzed acetic anhydride for different lengths of time and exposed to Tyromyces palustris, Serpula lacrymans, Coriolus versicolor and unsterilized soil. The action of OH-radical on acetylated wood was also examined using Fenton's reagent. The enhancement of decay resistance by acetylation was revealed clearly for all cases of exposures but varying with fungal and wood species used. For a brown-rot fungus Tyromyces palustris, the weight loss reached almost nil in all woods at 20 WPG (weight percent gain) of acetylation, after the striking decrease from 10 to 15 WPG. For a white-rot fungus Coriolus versicolor, it was counted until 12-15 WPG in the perishable hardwoods used, but not in a softwood Cryptomeria japonica, even at 6 WPG. In cases of another brown-rotter Serpula lacrymans and soil burial, effect of acetylation was intermediate between Tyromyces palustris and Coriolus versicolor. Anti-degradation mechanism by acetylation was discussed, from these weight loss - weight gain relationships, and the IR-and 13C-NMR spectral analyses of fungus-exposed wood.
M Takahashi, Y Imamura, M Tanahashi

Preliminary study of the fungicidal and structural variability in copper naphthenates and naphthenic acids
1996 - IRG/WP 96-30114
Copper naphthenates, an oil-borne wood preservative listed by the American Wood-Preservers' Association (AWPA), is manufactured by complexing copper(II) with naphthenic acids. Prior to AWPA listing as a wood preservative, field experiments showed that copper naphthenates generally had good stability and were active against wood-destroying organisms. Recently, however, there have been reports of some copper naphthenate-treated poles rapidly failing. One possible explanation for the varying effectiveness could be that the structure, and resulting biological activity, of the naphthenic acids used to make copper naphthenate may vary. To test this hypothesis several naphthenic acids and copper naphenates were obtained and their fungicidal activity against three wood-destroying fungi measured. In addition, the chemical structure of the naphthenic acids were examined by proton- and carbon- NMR. Different activities were observed, especially against a copper-tolerant fungus. Some apparent correlations were seen between the fungicidal activity and chemical structures for the few samples studied.
T Schultz, D D Nicholas, L L Ingram Jr, T H Fisher

The effect of certain wood extractives on the growth of marine micro-organisms
1977 - IRG/WP 438
S E J Furtado, E B G Jones, J D Bultman

IRG/COIPM INTERNATIONAL MARINE TEST - to determine the effect of timber substrate on the effectiveness of water-borne salt preservatives in sea-water. Progress Report 2: Report of treatment and installation in Australia
1978 - IRG/WP 440
The purpose of this test and the procedures to be followed have been fully set out in documents distributed by the International Research Group on Wood Preservation and numbered IRG/WP/414 and IRG/WP/420. The prescriptions set out in these two documents have been closely followed.
J Beesley

Synergistic effect of boron on Streptomyces rimosus metabolites in preventing conidial germination of sapstain and mold fungi
1992 - IRG/WP 92-1565
We evaluated the synergistic effect of boron (4% BAE solution of Tim-Bor or 4% boric acid) on Streptomyces rimosus metabolites in preventing spore germination of sapstain and mold fungi using plate bioassay, Southern yellow pine and sweetgum block tests, and green pine log sections: sapstain -- Ceratocystis coerulescens, Ceratocystis minor, and Aureobasidum pullulans; mold fungi -- Aspergillus niger, Penicillium spp, and Trichoderma spp. Inhibition of spore germination in plate bioassay by metabolites with boron was more effective than without added boron. Treatment of wood samples with the mixture of boron and unconcentrated metabolites also resulted in the synergistic effect and completely inhibited spore germination of sapstain and mold fungi.
S C Croan, T L Highley

Soft rot penetration - Effect of groundline maintenance treatment on poles in sevice
1983 - IRG/WP 3263
R S Johnstone

IRG/COIPM INTERNATIONAL MARINE TEST - to determine the effect of timber substrate on the effectiveness of water-borne salt preservatives in sea-water. 2nd Interim Report
1981 - IRG/WP 477
Three reference wood species - Alstonia scholaris, Fagus sylvatica and Pinus sylvestris, untreated and treated with 3%, 6% and 10% CCA and CCB solutions were supplied to all participants for submergence at local sites. Regular examination of samples is being carried out - 6 months, 12 months and then annually for 7 years.
R A Eaton

Effect of medium-term degradation of beech wood by erosive (Phanerochaete chrysosporium) and lignin-selective (Ceriporiopsis subvermispora) strains of white rot fungi on its selected physical properties
2004 - IRG/WP 04-40292
At the Faculty of Wood Sciences and Technology a fungal delignification of normal and tension beech wood by erosive and lignin-selective strains white-rot fungi has been studied. The pre-treatment of both kind of wood samples was accompanied by partial delignification and apparent changes of their physical properties influencing the polar liquids penetration.
R Solár, S Kurjatko, M Mamonová, J Hudec

Fungicidal activity of some organic solvents, copper carboxylates and their complexes with 2-aminoethanol
1997 - IRG/WP 97-30136
We evaluated the activity of eight organic solvents against wood - rotting fungus Trametes versicolor in order to choose the most appropriate one for rapid screening tests of some copper(II) carboxylates and their adducts with 2-aminoethanol. Their activity against the selected fungus was classified in the following order: chloroform > N,N-dimethylformamide > acetonitrile > methanol > dimethyl sulfoxide > ethanol > acetone. The non-polar white spirit did not dissolve in the growth medium and the results could not be directly compared with the results for other solvents. As an appropriate solvent for screening of the tested copper(II) carboxylates, dimethyl sulfoxide (DMSO) was chosen. Minimal inhibitory concentration against Trametes versicolor of the screened carboxylates was in the concentration range of 1x10-4 to 1x10-3 mol/l. Coordinated amine ligands slightly, and not significantly, decreased fungicidal properties of the tested carboxylates.
M Petric, F Pohleven

IRG/COIPM INTERNATIONAL MARINE TEST - to determine the effect of timber substrate on the effectiveness of water-borne salt preservatives in sea-water. Progress Report 13: Report of fourth inspection (36 months) in Australia
1981 - IRG/WP 481
This report presents the results of the fourth inspection of the IRG/COIPM International Marine Test specimens installed in Sydney Harbour in December, 1977. The inspection took place on 9th December, 1980, after 36 months exposure. Full details of the treatment and installation of the specimens, as well as results of the first three inspections (after 6, 12, and 24 months exposure), have been presented in earlier reports. All inspections of this test have been carried out in accordance with the Working Plan (see IRG/WP/414) except for an X-ray examination of the specimens. This facility is not available at the test station. In conformity with the provisions of the Working Plan, the fifth and sixth replicates of the whole test (i.e. those specimens with '5' or '6' as the final digit of their serial number) were recovered and returned to the United Kingdom after 6 months and 24 months of immersion. Thus, only four replicates remain to be examined at this and ensuing inspections.
J Beesley

The biostatic effect of copper on decay of fire retardant-treated mining timber
1991 - IRG/WP 1507
Blocks of Eucalyptus grandis were treated with 20kg/m³ ammonium sulphate as fire retardant and challenged with Coriolus versicolor. Replicates were soil buried. A second set of blocks was treated with retardant and copper at 6.6 kg/m³ (ie 1% w/w), and challenged similarly. After 8 weeks weight losses produced by Coriolus versicolor in untreated, retardant treated and copper supplemented blocks were 45, 25, and 0% respectively, and corresponding weight losses in soil were 27, 25 and 10%. These results, and electronmicroscopical observations, showed conclusively that Eucalyptus grandis treated with fire retardant was rapidly decayed, and that copper inhibited such decay.
G D Shelver, E A Shelver, A A W Baecker

The evaluation of synergistic effects of chemicals on fungicidal efficacy in crossed-paper tests
1991 - IRG/WP 2383
The mixing effects of wood preservatives were evaluated using the crossed-paper technique. Two filter paper strips (0.7 x 8 cm²) were treated by soaking with different chemicals [fungicides, a termiticide (chlorpyrifos or phoxim), a surface-active agent, a synergistic agent, and a stabilizer], and placed at right angles to each other on a fully grown mycelial mat of a test fungus in a Petri dish. When the four organoiodine fungicides were incorporated with chlorpyrifos or surface active agent, only 3-iodo-2-propynyl butyl carbamate (IPBC) showed the desirable synergistic effect against every wood-decaying fungus tested. Other fungicides did not always tend to produce the synergistic effect with the addition of a surface active agent. 4-Chlorophenyl-3-iodopropargyl formal (IF-1000) appeared to indicate an undesirable antagonistic effect when mixed with either chlorpyrifos or a surface active agent. 3-Bromo-2, 3 diiodo-2-propenylethyl carbamate (EBIP) did not show any synergistic action by mixing with chlorpyrifos and/or a surface active agent, although the fungicidal enhancement was induced satisfactorily by mixing the fungicide with chlorpyrifos, a stabilizer and/or a synergistic agent, especially against Tyromyces palustris and Coriolus versicolor. Similarity of the results obtained in the present investigation and in the previous laboratory decay tests leads to the conclusion that the crossed-paper technique is suitable for the evaluation of the mixing effect of chemicals on fungicidal efficacy.
Dong-heub Lee, K Tsunoda, M Takahashi

CCA modifications and their effect on soft rot in hardwoods
1982 - IRG/WP 3201
Decay tests were carried out on wood samples treated with three waterborne compounds all identical in composition but applied in different forms. There were significant differences in the effectiveness of the treatments particularly as regards the control of soft rot.
S M Gray, D J Dickinson

The proposal for the method of testing the phytotoxic effect of wood preservatives
2000 - IRG/WP 00-50163
Four methods of testing the phytotoxic effect of wood preservatives are proposed as the unified test by: - direct application of the preservatives to the test plants by spraying; - direct application of the preservative to the soil substrate; - indication plants growing in the vicinity of treated wood; - seed germination rate on the treated substrate.
J Wazny, P Witomski

Effect of water repellents on leaching from CCA treated wood
1995 - IRG/WP 95-50044
CCA treated fence boards brushed with a water repellent finish had consistently lower leaching losses of all CCA components compared to the rate for matched samples without the water repellent. These results are after 12 cycles of simulated rainfall in the laboratory (1800 mm rainfall total) and four months of natural rain exposure in Toronto.
P A Cooper, R MacVicar

Fungicidal combination products
1987 - IRG/WP 3426
Due to the increased pressure on some of the established fungicides used in wood preservation, possible alternative products become more interesting. The requirements for new chemicals are mainly lower toxicity and greater environmental acceptability. However the efficacy to target organisms should be as good as that of the currently used ones, preferably better. A possibility for progress in this direction could be fungicidal combination products showing broader spectrum of efficacy and synergistic effects. Mixtures of tributyltin compounds with Furmecyclox and K-HDO respectively are tested for this purpose. Toxic values with and without artificial ageing (wind-tunnel exposure and leaching) were determined. Investigations have been made with coating-formulations in order to test penetration, evaporation and the influence of UV-radiation. Aqueous formulations were tested for special purposes such as the treatment of freshly cut timber and the protection of brickwork. The results obtained are very promising, especially regarding long term durability. Further investigations mainly with the aqueous formulations including other test fungi and field trials are necessary to confirm the suggested application as wood preservatives.
H A B Landsiedel

Effect of protective additives on leachability and efficacy of borate treated wood
2002 - IRG/WP 02-30290
Borate preservatives have been used extensively in many countries as an effective means for protecting wood against fungal and insect attack especially in interior environments. Under exterior conditions, borate compounds have a main disadvantage as they can be leached from treated wood as a result of their water solubility. In this study, we compared the potential of different additives for reducing the leachability of boron preservatives from treated wood. Scots pine sapwood (Pinus sylvestris) and poplar (Populus trichocarpa x deltoides) test samples were vacuum treated with 1 % BAE (Boric Acid Equivalent) disodium octaborate tetrahydrate (DOT) solutions containing various additives e.g. glycerol/glyoxal, polyvinylpyrrolidone (PVPY), a commercial resin compound and a commercial water repellent. The European Standard EN 84 was used as a leaching test for both coated and uncoated specimens. The results of chemical analysis of leachates taken at different periods showed that the use of protective additives reduces the boron leachability. The glycerol/glyoxal additive applied to treated pine sapwood showed the best performance. The percent of boron retained in uncoated pine sapwood was 26% while coated samples still retained 45% after 14 days of intense leaching. Similar tests on poplar revealed 19% and 34% for uncoated and coated samples, respectively.This represents a gain of 20 to 25% compared to pure DOT treated specimens of both wood species. Preliminary biological tests were carried out on malt agar using a miniblock technique for uncoated pine sapwood and beech, with Poria placenta and Coriolus versicolor, respectively. After six weeks of exposure to fungal attack all boron protective systems tested proved their effectiveness, as none of the test samples exhibited a mass loss exceeding 4%. The reference 1% BAE without protective additives showed an average mass loss of 15%. Finally, test data are reported of standard EN 113 testing in view of a further evaluation of the biological efficacy of combined DOT-additive treatments.
A Mohareb, J Van Acker, M Stevens

Vertical distribution of fouling and wood-boring organisms in the Trondheimsfjord (Western Norway)
1981 - IRG/WP 467
Results of a detailed study on the vertical distribution of fouling and wood-boring organisms of Trondheimsfjord at an interval of 3 m from intertidal level to a depth of 30 m, has been presented, based on data collected from two series of panels, exposed from 15 March 1977 to 15 July 1977 (Series I) and from 22 July 1977 to 13 March 1978 (Series II). The intensity of fouling generally decreased with increasing depth. Quantitatively, fouling was heavy on panels of Series I more than on Series II, although species-wise it was more heterogenous on panels of Series II. The bulk of the fouling was constituted by Balanus crenatus, Laomedia sp., Mytilus sp., Modiolus sp. and Hiatella arctica. Incidence of borers and the resultant destruction of timber were heavier on panels of Series II than on those of Series I. The influence of the period of exposure on the above pattern of infestation by foulers and borers has been discussed. Psiloteredo megotara concentrated at the upper levels up to a depth of 15 m, with more settlement between 3 m to 9 m depth. Although Xylophaga dorsalis was present on panels from 3 m to 30 m depth, their intensity abruptly changed from 9 m onwards and continued to increase with increasing depth, with a maximum number near the mud level at 30 m. Attack of Limnoria lignorum also was heavy at the mud level. On the same panel, while Psiloteredo megotara preferred to settle in more numbers on the lower surface, Xylophaga dorsalis did so on the upper silted surface. The importance of such selective vertical incidence of different borers and their co-operation in the destructive activity have been stressed from the point of wood destruction in the Tronheimsfjord. The rate of growth of Balanus crenatus, Laomedia sp., Psiloteredo megotara, Xylophaga dorsalis, and Xylophaga praestans in relation to depth has been presented. For Balanus crenatus rate of growth decreased with increasing depth, while for Laomedia the same increas with depth up to 12 m and declined thereafter. In accordance with its depth preference, Psiloteredo megotara registered faster growth between 3 to 15 m depth. In the case of Xylophaga dorsalis, the size of the shell valve and burrow increased with increasing depth up to 24 to 27 m and then showed a slight decline at 30 m. Factors influencing the growth-rate at different levels have been discussed. The results on the vertical zonation and rate of growth of the wood-infesting organisms encountered, have been compared with relevant literature published earlier.
L N Santhakumaran

Effect of a penta emulsion on the service life of Douglas fir, heartwood posts
1978 - IRG/WP 3112
C S Walters

IRG/COIPM INTERNATIONAL MARINE TEST - to determine the effect of timber substrate on the effectiveness of water-borne salt preservatives in sea-water. Progress Report 10 from Naos Island, Panama
1980 - IRG/WP 462
Blocks of 3 wood species, Beech (Fagus sylvatica), Scots pine (Pinus sylvestris) and Alstonia (Alstonia scholaris) were exposed at site number 12 at Naos Island, Panama on March 8, 1978 by John R. DePalma. The arrangement of the panels in the exposure site is as shown in Figure 1.
D W French

The effect of treatment method on CCA efficacy in Corsican pine
1992 - IRG/WP 92-3723
As part of a study into the influence of application method on preservative efficacy Corsican pine (Pinus nigra) samples (50 x 50 x 400 mm³) were treated with a CCA formulation using Bethel, Steam/Bethel or Lowry processes. Full penetration of the preservative at a gross level was confirmed using a copper disclosing reagent. The preservative was allowed to fix and then samples were converted into mini-blocks (30 x 10 x 5 mm³) to produce decay test samples from various locations within the larger samples. After leaching, sets of replicate mini-blocks were exposed to the decay fungi Coniophora puteana FPRL 11E, Coriolus versicolor FPRL 28A, and Chaetomium globosum FPRL S70K. Equivalent sets of leached blocks, were analysed using atomic absorption spectrophotometry to determine preservative concentration and balance. The results of this study have been used to assess the effect of preservative application method on CCA efficacy. They also indicate how treatment method affects the distribution of the active elements of the preservative throughout the treated wood.
P R Newman, R J Murphy

IRG/COIPM INTERNATIONAL MARINE TEST - to determine the effect of timber substrate on the effectiveness of water-borne salt preservatives in sea-water. Progress Report 8: Panama test results
1980 - IRG/WP 458
Summary of damage to ITRG test stakes by pholadidae and teredinidae at the Panama test site - 8 Mar. '78 to 11 Oct. '79
J R De Palma

Changes in the degree of decay of lignocellulosic substrate used in a screening test of fungicidal wood preservatives
1977 - IRG/WP 287
This report contains results of investigations aimed at: a) determination of the effect of the kind of substrate and species of test fungus on quantitative changes in used samples prepared from spruce cardboard, and b) comparison of the threshold fungicidal values of come fungicides determined with accelerated method, with values obtained by block method. During performed investigations, the method described in Document No.: IRG/WP/262 was used. Assesment of decomposition degree was based on the loss of weight and amount of NaOH consumption by the substrate.
K Lutomski

Boron treatments for the preservation of wood - A review of efficacy data for fungi and termites
1994 - IRG/WP 94-30037
Boron treatments have been used for many decades for protection of timber from biological attack and also as a fire retardant treatment. In recent years there has been an increased interest in boron treatments as an option for protection of structural timbers' e.g. timber framing used in termite risk areas. This paper reviews efficacy data for both fungi and termites relevant to this end-use.
J A Drysdale

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