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The influence of previous anti-blue-stain preservative treatments on the fixation of CC in spruce
1997 - IRG/WP 97-30134
Freshly cut and kiln dried spruce boards were treated with 4 different anti-blue stain preservatives (ABP). After a period of 10 days allowing the samples to dry and fixate, the samples were treated with CC (chromium, copper formulation) using a vacuum pressure cycle. After impregnation the wood was steam fixed. A submersion leaching test showed differences in the leachable quantity of copper and chromium. When related to the retention of both salts after impregnation, no differences could be found between samples previously treated with anti-blue-stain preservatives and untreated samples. However, the retention of CC diminished significantly by application of quaternary ammonium compounds.
M Van der Zee, W J Homan

Fungicidal properties of boron containing preservative Borosol 9
2004 - IRG/WP 04-30348
The fungicidal properties of new boron containing wood preservative Borosol 9 is described in this paper. These properties were of particular interest as this new boron containing preservative, exhibit very good performance against wood damaging insects. But because the tested boron formulation contain also nitrogen compounds, we wanted to verify if nitrogen as a nutrient could promote growth of wood rotting and blue stain fungi. Fungicidal activity of the boron based wood preservative Borosol 9 was evaluated according to the standard EN 113 procedure. Samples made of Norway spruce were brushed two times with 10% aqueous solution of Borosol 9, air dried, steam sterilized and exposed to the following wood rotting fungi: Coniophora puteana, Gloeophyllum trabeum and Lentinus lepideus. After 16 weeks of exposure the specimens were isolated and their mass losses were determined. After this respective period, the treated wood samples lost on an average less than 1 % of their initial mass. Parallel to this experiment, blue stain testing according to the EN 152-1 procedure was performed. Specimens brushed with Borosol were for six weeks exposed to blue stain fungi Aureobasidium pullulans and Sclerophoma pithyophila. After testing period the specimens were isolated and anti blue stain efficacy was determined visually. Both tests showed that Borosol 9 has fungicidal properties. Preservative, containing the boric acid - alkanolamine complex did not enhance fungal decay or growth. On the contrary, they showed high activity against wood decay and blue stain fungi.
G Babuder, M Petric, F Cadež, M Humar, F Pohleven

A summary of work carried out to compare natural and artificial weathering for preconditioning test specimens in testing anti-stain chemicals for wood in service
1976 - IRG/WP 266
Since 1972 work has been in progress within CEN to establish a European standard for the testing of wood preservatives designed to prevent blue-staining of wood in service. The method is based on the Butin test and involves pre-weathering of the samples prior to biological testing. The weathering regime employed is 6 months outside exposure from March to October. This imposes severe limitations on the number of tests that can be carried out each year and has prompted considerable interest in an artificial weathering procedure which can be used instead of natural weathering. Recently four European laboratories (EMPA, BAM, IC, PRL) have been involved in assessing artificial systems (Xenotest, Marr, Gardener wheel, weathering tunnel) and this report has been prepared at the request of CEN in order to assess the accumulated data and make recommendations back to the CEN. This document presents only a brief account of the work and fuller details can be obtained directly from the laboratories concerned.
D J Dickinson, A F Bravery

Comparison between two laboratory test methods for determining the effectiveness on wood preservatives against blue stain in fresh wood
1987 - IRG/WP 2289
Most of the work done on determination of the effectiveness of new formulae for treating fresh wood against blue stain have been focussed on their use in the manufacture of saw timber. This work explains two laboratory methods, one which simulates the working and climatological conditions of factories making packages for fruit and vegetables in the Spanish Levante, showing that contamination of wood is caused naturally, and another method causing blue stain by innoculation with pure cultures. Both methods were applied to eight preservatives, and the results were compared.
A M Navarrete, M T De Troya

The use of preservative containing waste wood as substrate for growing greenhouse crops
1993 - IRG/WP 93-50011
In the Netherlands a large amount of waste wood and wood waste is produced every year. An important part of this amount comes from the pallet and packaging industries. One of the possibilities to re-use this relatively clean material is to convert it into substrates for growing crops in glass houses instead of the commonly used materials such as rock wool and glass wool. In this research, the influence of several material parameters such as wood species, texture, density, height, water holding capacity on the growth of cucumbers has been studied and this has been compared with the growth on rock wool, which is applied in approximately 95% of the glasshouses in the Netherlands. Furthermore, the influence has been investigated of anti blue stain preservatives on the growth of the cucumber plants, this kind of preservatives is often used in the dutch industry for protection of pallets. In general it can be concluded that when waste wood is to be used as substrate a few preservatives can be accepted and some others can not. The wood species teture, density, height and water holding capacity of the substrate showed to have only a slight effect on the growth of cucumbers.
W J Homan,H Militz

Performance of Tuff Brite C™ and other formulations against blue-stain, mold and brown-stain in freshly-sawn rubberwood (Hevea brasiliensis) in the humid tropics of Peninsular Malaysia
1997 - IRG/WP 97-30163
The relative anti-sapstain and anti-mold efficacies, including brown-stain development in freshly-sawn rubberwood (Hevea brasiliensis) in the Malaysian tropics, between selected water-based product concentrations of formulations Tuff Brite CTM without/with added Borax (at 1.5/0, 1/1.5, 1.5/1.5 and 2%/1.5%), PQ8TM without/with added Borax (at 2.5/0, 1.5/1.5, 2/1.5 and 2.5%/1.5%) and NeXgenTM/NeX-BriteTM combination (at 1/0.25, 1.5/0.25 and 2%/0.25%) were assessed visually. Rubberwood boards, dipped in these concentrations for 1 min. were subjected to an 8 weeks sapstain trial in a FRIM sawmill shed. Sodium pentachlorophenate/Borax combination (at 2/1.5 and 2%/2%) was the reference anti-sapstain chemical. The rough-sawn boards were rated for blue-stain and mold weekly, while both the planed and the internal faces of the boards, sawn lengthwise into two-halves, were rated for blue-and brown-stain only after 8 weeks. Results for the rough-sawn boards, planed boards and the internal faces of the boards revealed a generally consistent excellent performance of Tuff Brite CTM without/with Borax against blue-stain and mold in rubberwood, protection being marginally better than that of NaPCP/Borax. Over a range of concentrations, performance by the remaining fomulations PQ8TM without/with Borax and NeXgenTM/NeX-BriteTM were variable: rated satisfactory (generally <10% surface infected) and/or poor (31-50% of surface infected) against sapstain and/or mold. Against brown-stain development (i.e. to conserve the natural cream/yellow hue in rubberwood), Tuff Brite CTM without/withBorax also appeared to be relatively better (generally yellow-to-light brown hue) than the other formulations, but perhaps only marginally so than NaPCP/Borax (light blue as well as yellow-to-light brown hue observed).
A H H Wong, T L Woods

A field test with anti-stain chemicals on sawn pine timber in Brazil
1989 - IRG/WP 3513
Field tests were carried out in São Paulo State - Brazil, to evaluate pre-treatment formulations of O-Phenyl-Phenol (OPP) based products. The Sodium Pentachlorophenate solution was used as a reference formulation. The treated test blocks were stacked in a covered shed, and in an uncovered place for air drying. The evaluation was done by measuring the percentage of stained surface on each test block, and the effect of the sodium tetraborate on the OPP fixation was also tested. Statistics analyses allowed to correlate the factors exposition, treatment, among others, indicating that the OPP 2.1% plus sodium tetraborate, showed the best performance.
M B B Monteiro, S Brazolin

Methods of testing anti-stain chemicals for protecting sawn timber during storage and transport
1976 - IRG/WP 273
No international standard method for testing anti-stain chemicals for protecting fresh sawn timber exists. The methods used can be divided into three types: a) Rapid screening tests for finding chemicals effective against blue-stain and mould fungi. The chemicals are tested in agar medium, in pieces of filter paper or on small pieces of wood using pure cultures. b) Laboratory tests for evaluating the effectiveness of a preservative and estimating the minimum concentration for the treating solution. Wooden test pieces of different size and artificial infection are used. c) Field tests for evaluating the effectiveness and performance of a preservative under practical conditions. The fungal infection is mostly natural. There is a need for standardisation of the method especially for the tests of type b, to be able to repeat the tests and have comparable results. The results from field tests are always dependant upon the natural conditions that vary between places and even in the same place. Besides, a field test often tries to simulate the local service conditions. However, standardisation of the techniques and of the principle of evaluation might faoilitate the conduction of tests, and the comparison of the results. In this paper, a short summary is made on the variables in the present tests, that should be subject to unification. The summary is based on literature, that is not complete. The differences in the present methods are so numerous that they cannot be discussed here in detail.
T Vihavainen

Protection of pine sawtimber and sawn timber against blue stain in Poland
1983 - IRG/WP 3193
The purpose of this paper is the synthesis of more important results of basic and applied research that have been grouped in the following problems: 1.: Fungi causing blue stain in the pine wood, occurring in Poland. 2.: Moisture content changes of pine sawtimber and sawn timber as the factor of blue stain producing fungi development. 3.: Effect of sapwood moisture content on the degree of wood staining in dependence on wood preservation methods and sawn timber quality. 4.: Effect of pine sawtimber harvesting period and storage time on sawn materials quality. 5.: Protection of pine sawtimber and sawn timber with chemical compounds. 6.: Problems of economics of sawtimber and sawn timber preservation.
E Tarocinski, M H Zielinski

Report on the activities of the European Standardization Committee CEN/TC 38 'Methods of Testing wood preservatives'
1980 - IRG/WP 279 E
G Castan

Blue stain in service on wood surface coatings. Part 3: The nutritional capability of Aureobasidium pullulans compared to other fungi commonly isolated from wood surface coatings
1993 - IRG/WP 93-10035
The nutritional capability of Aureobasidium pullulans was previously examined, using agar plate tests, with regard to nutrient sources that are potentially available in fresh and weathered wood (Sharpe and Dickinson, 1992). This study compared these findings with the nutritional capability of four other fungi (Alternaria sp., Cladosporium cladosporoides, Stemphylium sp. and Trichoderma sp.) commonly isolated from wood surfaee coatings. The liquid culture techniques were used to assess the relative abilities of the fungi to utilise a range of simple sugars, wood sugar alcohols, hemicelluloses, cellulose and lignin degradation compounds. The observations were used to explain why Aureobasidium pullulans is able to occupy so successfully, often in monoculture, the wood-paint interface niche.
P R Sharpe, D J Dickinson

Mould resistance of lignocellulosic material treated with some protective chemicals
1984 - IRG/WP 3294
Effectiveness of preserving lignocellulosic material against moulding by treatement with water solutions of commercial wood preservatives and mixtures of various inorganic salts was investigated and compared with the effectivenes of sodium pentachlorophenoxide and boric acid.
K Lutomski

Proposed standard laboratory method for testing fungicides for controlling sapstain and mould on unseasoned lumber
1977 - IRG/WP 292
This laboratory method is for determining the effective concentration, or concentration for zero growth (CGo), for fungicides or preparations of fungicides which are potentially useful in protecting packaged or unseasoned lumber in storage and shipment from biodeterioration by sapstain fungi and moulds. The test is rapid and may be completed in three weeks and gives a good indication of the toxicity of a chemical against sapstain fungi and moulds.
A J Cserjesi

Field trials of anti-sapstain products. Part 1
1991 - IRG/WP 3675
The results obtained in two field tests of anti-sapatain products, carried out in four locations in Portugal, are presented. Boards from freshly cut logs were hand-dipped, close staked and left to dry for periods from four to six months. The results obtained seem to indicate that some of the products tested performed at least as well and sometimes better, than a 3% NaPCP solution which was used as control product.
L Nunes, F Peixoto, M M Pedroso, J A Santos

Evaluation of the effectiveness of three microbiocides in the control of sapstains
1982 - IRG/WP 3212
Results of field test on the effectiveness of BUSAN 30, CAPTAN, FOLPET against mould and sapstain in Pinus elliottii are presented. The viability of use of FOLPET in Brazil as an alternative to sodium pentachlorophenate is also discussed.
S Milano, J A A Vianna Neto

Tebuconazole, a new wood-preserving fungicide
1990 - IRG/WP 3634
TEBUCONAZOLE, an anti-fungal triazole compound, has been tested to assess the effectivness as wood preserving fungicide. Tests were made with the active ingredient and also in formulations against basidiomycetes, blue-staining fungi and mould. Results of the DESOWAG laboratories and also from official institutes will be presented. In addition to this there were made different toxicological and ecotoxicological trials according to OECD and EPA guidelines
B Wüstenhöfer, H-W Wegen, W Metzner

Rapport sur l'activité du CNE/TC 38 'Méthodes d'essais des produits de préservation du bois'
1980 - IRG/WP 279
G Castan

Blue stain in timber in service. Results of co-operative tests to compare different artificial weathering systems 1981-82
1983 - IRG/WP 2193
The paper describes results of the third phase of co-operative laboratory experiments comparing the effects of different artificial weathering systems on chemicals to control blue-stain in service. Atlas, Xenotest and Marr equipments are shown to give essentially the same results for 5 of the 6 chemicals tested.
A F Bravery, D J Dickinson

Blue stain in service on wood surface coatings. Part 2: The ability of Aureobasidium pullulans to penetrate wood surface coatings
1992 - IRG/WP 92-1557
The ability of Aureobasidium pullulans to penetrate seven different wood surface coatings was examined. Eight isolates of Aureobasidium pullulans were used. Following penetration, light microscopy was used to examine the effect of the fungus upon the coating, and growth at the wood-film interface. The fungus showed different growth capabilities with different types of surface coatings. Some isolates were able to penetrate alkyd based coatings, but were unable to grow in the film. In contrast, after penetration through latex based coatings several isolates were able to produce growth in the films themselves.
P R Sharpe, D J Dickinson

Rapport sur l'activité du CEN/TC 38
1983 - IRG/WP 2204
M Pottevin

The identification and preservative tolerance of species aggregates of Trichoderma isolated from freshly felled timber
1992 - IRG/WP 92-1553
The surface disfigurement of antisapstain treated timber by preservative-tolerant fungi remains a major problem in stored timber. Identification of a range of isolates of Trichoderma based on microscopic morphological characteristics was found to be imprecise due to the variable nature of this organism. In addition, studies to compare visual (morphological) characteristics of these isolates with their tolerance to the antisapstain compound methylene-bis-thiocyanate (MBT) using minimum inhibition concentration (MIC) tests showed no clear correlations. Isoenzyme electrophoresis was used to investigate the taxonomic relationships between species aggregates of Trichoderma isolated from antisapstain field trials and to identify physiological differences between 30 isolates of Trichoderma which show tolerance to MBT at concentrations ranging from less than 4 ppm to 34 ppm. Results indicate that there is considerable variability in the preservative tolerance of different Trichoderma isolates from particular locality. This highlights the need for field testing of an antisapstain compound in the same locality and under the same conditions in which it will be used in practice.
R J Wallace, R A Eaton, M A Carter, G R Williams

Working Group II: Sub-group: Methods of testing anti-stain chemicals for protecting sawn lumber during storage, transit
1978 - IRG/WP 2121
R Smith

Blue-stain fungi (Ceratocystis spp.) found in Spain on pine woods
1989 - IRG/WP 1410
So far, there is only a very limited reported description of the different Ceratocystis spp. present on fresh wood in Spain. So, the main goal of this work has been the identification of species of this genus causing blue-stain on Pinus pinaster A. Ait. and Pinus sylvestris L. woods. We have also investigated the relationship between the species found and their propagation vectors (insects and wind). Finally, we have determined the growing velocity of two of the most representative species found and the presence or absence of degradative enzymatic activities.
M T De Troya, A M Navarrete

Rapport sur l'activité du Comité CEN/TC 38 "Méthodes d'essais des produits de préservation des bois" du Comité Européen de Normalisation (CEN)
1986 - IRG/WP 2266
G Castan

Rapport sur l'activité du CNE/TC 38 'Méthodes d'essais des produits de préservation du bois'
1977 - IRG/WP 288
G Castan

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