IRG Documents Database and Compendium


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Using an accelerated laboratory method to evaluate Aureobasidium pullulans colonization on painted surfaces and underlying wood substrate
2012 - IRG/WP 12-20480
Mold growth on painted surfaces can lead to premature paint film failure and is unsightly. Aureobasidium pullulans has been identified as a major cause of black stain on surfaces painted with acrylic or latex paints. In this study, an accelerated laboratory method developed by Zabel and Horner (1981) was used with modifications to the type of agar used and sample evaluation methods. A study was ...
C Schauwecker, L Burns, A Preston


The role of hydrophobins in surface growth by the Blue Stain fungi Aureobasidium pullulans
2015 - IRG/WP 15-10837
Hydrophobins are small proteins found exclusively in filamentous fungi. These proteins are able to assemble spontaneously into amphiphilic monolayers at hydrophobic–hydrophilic interfaces. Hydrophobins have a diverse role, e.g. allowing the fungi to break through interfaces during aerial hyphae formation, sporulation, fruit body production and cell penetration. In this ongoing study, we are look...
J Stenbæk, L Riber, J Blæsbjerg Nielsen, C Møller Hansen, B Jensen


Effects of UV Radiation on Melanization and Growth of Fungi Isolated from Weathered Wood Surfaces
2015 - IRG/WP 15-10842
This research tests the hypothesis that fungi isolated from weathered wood will respond to elevated levels of UV radiation by increasing their melanin production. To test this hypothesis, we measured the melanin production and mycelial color of Aureobasidium pullulans and Cladosporium cladosporioides, before and after the fungi were grown under artificial UV, visible light and complete darkness. T...
V A Hernández, P D Evans


Detection of semi-quantitative and qualitative enzymatic activities of blue-stain fungi
2000 - IRG/WP 00-10347
Blue-stain, produced in forests, continues to be a problem in countries of moderate climate. In forestry, in certain geographical areas of Spain, it has been observed that anti-sapstain products have not always been efficient, as their fungicidal effectiveness varies on occasion, depending on the species of wood and microbiota that exist in the region. It is not always easy to identify the species...
M T De Troya, F Llinares, D Muñoz-Mingarro, M J Pozuelo, N Acero, C Rodríguez-Borrajo, A M Navarrete


Preventive effectiveness of petrol derivatives against blue-stain fungi
1992 - IRG/WP 92-3716
A possible application of fractions obtained on petrol pyrolysis is their use as a wood preservative against wood-decay and sap-stain fungi. From two of these fractions were prepared three different products (ST, STL and SST). The aim of this work has been the possibility of utilizing these products in the treatment of wood used for external joinery, determining their effectiveness against blue-st...
A M Navarrete, M T De Troya, E Sanchez, A Guijarro


Distinguishing isolates of Aureobasidium on the basis of their ability to utilise lignin breakdown products as a sole carbon source
1996 - IRG/WP 96-10151
Wild isolates of Aureobasidium were obtained by dilution plating from a ca 20 year old painted pine window frame. Four isolates were obtained from the paint/wood interface region and four isolated from regions deep in the wood. The isolates were then used to inoculate liquid cultures containing ferulic acid, a lignin breakdown product, present as the sole carbon source. Ability of the isolates to ...
M W Schoeman, D J Dickinson


Aureobasidium or Hormonema? A Genetic Approach.
2004 - IRG/WP 04-10529
Aureobasidium pullulans is the main organism causing disfigurement of coatings on wood and the surface of exposed timber. This disfigurement of timber in-service is referred to as “bluestain in-service”. A. pullulans is also associated with the sapstaining of dead wood in the forest and in-service. A. pullulans is noted for its highly variable growth forms (polymorphisms). This variability pre...
M J Ray, D J Dickinson, M Buck


The importance of blue stain attack for the colonization by wood-rotting fungi of wood not in contact with the ground
1988 - IRG/WP 1349
When used in constructions not in contact with the ground, wood has been shown often to proceed from blue stain to moulds. The appearance of wood rotting fungi is normally delayed. Solid wood artificially inoculated with the blue stain fungus Pullularia pullulans was shown to permit germination of Pycnoporus cinnabarinus basidiospores. This was demonstrated by the use of an indirect and a direct b...
J Bjurman


Dominant genera of fungi isolated from the surfaces of Sugi (Cryptomeria japonica D. Don) heartwood lumbers exposed at six test sites from northern to southern regions of Japanese islands
1999 - IRG/WP 99-10304
The surfaces of wood materials are disintegrated not only by sunlight and rainwater but also by microbes when exposed above ground condition. This paper deals with the investigation of fungi isolated from the surfaces of Sugi heartwood lumbers (W100 by T10 by L300 mm) exposed at an angle of 45° without ground contact for 16 months at the six test sites from northern to southern regions of Japan. ...
S Doi, M Mori, M Kiguchi, Y Imamura, M Hasegawa, S Morita, S Nakamra, Y Kadegaru


The Effects of Solar Radiation on the Fungal Colonization and Color of Weathered Wood
2008 - IRG/WP 08-10676
Solar radiation rapidly depolymerizes lignin at wood surfaces exposed outdoors producing a suite of low molecular weight aromatic compounds within weathered wood. Fungi colonizing weathered wood are able to metabolize these aromatic compounds, and many of them also contain high levels of melanin to protect themselves from the harmful effects of UV radiation. Both of these adaptations suggest that ...
V Hernandez, C Breuil, P D Evans


Detection of Aureobasidium as the dominant fungus on coated wood
2013 - IRG/WP 13-10796
Wood is often covered with a coating in order to protect the surface. Unwanted dark mold growth on paint covered surfaces in external conditions is a familiar phenomenon. Simple microbial techniques have commonly been used to determine the dominant fungal species present in the discoloured area, for example morphological analysis of microscopic preparations of fungal pieces sticking to an adhesive...
E J Van Nieuwenhuijzen, M F Sailer, R A Samson, O C G Adan


Susceptibility of wood substrates to Aurebasidium pullulans at different temperatures
2016 - IRG/WP 16-10863
There is an increasing awareness of how the aesthetical performance of wood exposed outdoors changes over time and especially in the first few years after installation. Mould and blue stain fungi are biological agents that contribute to the weather grey colour on a wooden façade, and the blue stain fungi Aureobasidium pullulans is commonly identified as colonizer on coated and uncoated wood expos...
L Ross Gobakken, G Alfredsen


Thermal stability of a wood protective biofinish and the influence of flame retardants on Aureobasidium cells
2017 - IRG/WP 17-30716
In general there is an increasing need for sustainable resources, including sustainable building materials. Wood is one of these sustainable resources and is more used as an outdoor building material. Since unprotected wood will usually be degraded during outdoor exposure, many different protection systems are available on the market. Protection systems containing biocides however might create obs...
S Rensink, E A M Klein Rot, M F Sailer


Effects of wood protecting biofinish and linseed oil on fire behaviour and leachability of the fire retardant
2018 - IRG/WP 18-30728
A wood protecting biofinish is based on a protective and decorative fungal-based coating and a linseed oil impregnation (called Xyhlo biofinish). This biofinish enables the long-term use of wood in outdoor applications without using toxic chemicals. The fire resistant properties of materials used in buildings are very important. Since Xyhlo biofinish is relatively new, only little information is ...
S Rensink, E J van Niewenhuijzen, M F Sailer


Biofinish: A Functional Wood Surface Treatment based on Aureobasidium
2020 - IRG/WP 20-40903
Xyhlo Biofinish is a natural and environmentally friendly wood protection concept based on linseed oil impregnation and a fungal-based coating. It emerged in the late 1990s and has been developed over the past 15 years into an industrially applicable process in the Netherlands. The combination of linseed oil impregnation and a surface treatment with the living fungus Aureobasidium extends the serv...
S Rensink, M Sailer, S Roukens, J Gerber, H van der Mel, K Potgieter, J Spit, R Bulthuis, C Struck, M Bennink


Comparison of culturable fungi found on two types of modified wood cladding
2023 - IRG/WP 23-11025
Modified woods are rapidly gaining popularity in the United States and are appearing on more public buildings. Because these are biocide-free products, they can support the growth of non-wood degrading fungi on their surfaces, many of which produce dark pigments and deface the cladding. Even growth of dark pigmented fungi on modified woods yields an even grey-black color, giving the materials a we...
G Presley, J Cappellazzi, B Held, R Blanchette


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