IRG Documents Database and Compendium


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The role of chitinase in bioprotectant activity against staining fungi
1996 - IRG/WP 96-10175
Chitin is an important structural component of the hyphae of many wood staining fungi and its disruption can lead to dramatic declines in their growth. A number of bioprotectants have been shown to produce chitinases in liquid cultures, but the role of these enzymes in bioprotection remains poorly understood. The levels of these enzymes was studied by inoculating ponderosa pine sapwood wafers with...
J Liu, J J Morrell


Biological control of internal decay in Scots pine poles: A seven year experiment
1992 - IRG/WP 92-1529
The interactions between key fungi were monitored, by non-destructive and destructive sampling methods, during the course of an experiment on the effectiveness of Scytalidium sp. and Trichoderma spp. for eliminating established infections of Lentinus lepideus (Fr. ex Fr.) Fr. in creosoted Pinus sylvestris poles. Although extensively isolated at the beginning and end of the experiment, Trichoderma ...
P I Morris, D J Dickinson, B Calver


Preliminary agar screening studies as the first stage in the development of a biological control system against basidiomycetes in ground contact situations
1995 - IRG/WP 95-10111
Preliminary screening of Trichoderma isolates as possible biocontrol agents for protection of wood in ground contact situations against basidiomycete decay was undertaken on three types of media. Media used were 3% malt extract agar (MEA); a low nutrient synthetic media with a carbon: nitrogen ratio typical of that found in softwoods (LNM); and a similar synthetic media where the amino acid profil...
E J B Tucker, A Bruce


Biological control of sapwood-inhabiting fungi by living bacterial cells of Streptomyces rimosus as a bioprotectant
1992 - IRG/WP 92-1564
The objective of this study is to evaluate the efficacy of antifungal activity of living bacterial cells for the protection of wood against sapwood-inhabiting fungi. The following sapwood-inhabiting fungi were selected: sapstain --Ceratocystis coerulescens, Ceratocystis minor, Ceratocystis pilifera, and Aureobasidum pullulans; mold fungi --Aspergillus niger, Penicillium spp, and Trichoderma spp. L...
S C Croan, T L Highley


Shorter-term biological control of wood decay in pre-seasoning pine roundwood as an alternative to chemical methods
1992 - IRG/WP 92-1555
Previous studies on the long-term control of decay in creosoted transmission poles, using Trichoderma and other antagonistic moulds, have met with limited success. However, it is possible biological control is more suited to control of decay on shorter time scales. An earlier study, focusing on pre-seasoning treatment of transmission poles showed that favourable porosity increases could be brought...
M W Schoeman, D J Dickinson


Assessment of the biocontrol potential of a Trichoderma viride isolate in a field trial
1998 - IRG/WP 98-10252
A field trial has been set up near Dundee, to assess the biological control potential of a Trichoderma viride isolate T60. This isolate has been shown in laboratory tests to be particularly effective in protecting wood against certain basidiomycete decay fungi. Wood was treated with T60 spores using vacuum-pressure impregnation in a pilot preservation plant. Scots pine and Sitka spruce stakes wer...
H F Brown, A Bruce


The control and pretreatment decay in air seasoning Scots and Corsican pine poles in England
1990 - IRG/WP 1451
Previous work clearly showed that air-seasoned, British grown Scots and Corsican pine poles are subject to serious pretreatment decay. In order to control these infections a range of pretreatments are currently being investigated. One series of treatments is designed not only to control decay but also allow the development of mould and stain fungi in order to achieve the desired increases in poros...
D J Dickinson, A R Zahora, A P Dodson


Identification and inhibitory effect of volatiles from different ages of a Trichoderma aureoviride culture on selected wood decay fungi.
1995 - IRG/WP 95-10110
The ability of a Trichoderma sp. to produce volatile organic compounds (VOCs) over a four week period of growth was examined and the inhibitory effect of these volatiles against four selected basidiomycetes over the same period was assessed. After trapping, on tubes filled with chromatography packing material, VOCs were analysed on an integrated automated thermal desorbtion mass spectrometer syste...
A Bruce, A Kundzewicz, R E Wheatley


Comparative study of lignocellulolytic activities of Pleurotus spp. and white rot and brown-rot fungi
1997 - IRG/WP 97-10209
Field and fungal cellar trials have been set up to assess the biocontrol potential of a selected Trichoderma viride isolate in a situation representative of the end use of treated timber in ground contact situations. These trials are designed to give information about the efficacy of biological control as well as the suitability of existing chemical treatment methods for use with biocontrol fungi....
H L Brown, A Bruce


The effect of chitosan on the growth and physiology of two wood inhabiting fungi
2006 - IRG/WP 06-10590
The effect of chitosan on the wood inhabiting fungi Sphaeropsis sapinea and Trichoderma harzianum was evaluated at cellular level. Increasing concentrations of chitosan caused an increase in the amount of peroxide in cultures of S. sapinea, which was accompanied by a simultaneous decrease in superoxide. The same effect was not observed in T. harzianum. The growth of both fungi was inhibited when h...
D Vesentini, T Singh


Effect of chitosan on the morphology and ultrastructure of two wood inhabiting fungi
2007 - IRG/WP 07-10606
An investigation was undertaken to compare the antifungal effects caused by chitosan against two selected wood degrading fungi Sphaeropsis sapinea and Trichoderma harzianum on their growth, morphological and ultra-structural characteristics, using differential interference microscopy, epifluorescence microscopy and transmission electron microscopy. The effect of chitosan on fungal morphology was ...
T Singh, A P Singh, G Daniel


In vitro evaluation of an integrated approach using Trichoderma harzianum and chitosan for the control of sapstain
2008 - IRG/WP 08-10659
In vitro assays were undertaken to evaluate the control of two sapstain fungi, Leptographium procerum and Sphaeropsis sapinea by a combination of chitosan or chitosan oligomer (CODP-14) and a naturally mutated strain of Trichoderma harzianum. Spore germination and hyphal growth of the test fungi were assessed on media amended with chitosan or chitosan oligomer with and without T. harzianum using ...
C Chittenden, T Singh


Biological control of the sapstain fungus Diplodia pinea (syn. Sphaeropsis sapinea) in the field
2008 - IRG/WP 08-10663
Diplodia pinea, syn. Sphaeropsis sapinea, is well known as the worldwide ocurring causal agent of the Diplodia tip-blight disease on various pine species, leading to great losses of valuable wood in the forests. Moreover, this fungus is also responsible for the fornation of sapstain of felled logs, leading to undesired disation of surface layers in the OSB manufacturing industry. In Central Europe...
C Gradinger, T Boisselet, K Messner, K Fackler


Assessment of antagonism between lignicolous microorganisms: Research on possible use to preserve wood poles
2010 - IRG/WP 10-10718
Biological environments contain a certain number of microbial populations which, within a given ecological niche, display various relations ranging from symbiosis to parasitism. Researchers have been interested in these types of relations for around fifty years, especially in one very particular type of relationship: the antagonism exerted between individuals of the same microbial population. Toda...
J-F Labrecque, A Zaremski, L Gastonguay, Y Prin


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