IRG Documents Database and Compendium


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A rapid field bioassay technique with subterranean termites
1983 - IRG/WP 1188
Details are summarised of a field procedure which is designed to ensure continuous exposure to a replenishing termite biomass. After pre-baiting to determine the presence and identification of a termite hazard, test specimens (35 x 35 x 250 mm³) are installed vertically in the ground adjacent to and in contact with bait specimens of the same dimensions and interconnected by susceptible feeder str...
C D Howick, J W Creffield


International collaborative laboratory comparison of two wood preservatives against subterranean termites: Third update and first report
1996 - IRG/WP 96-10174
At the 24th annual meeting of IRG in Orlando, USA, in May 1993 an international subterranean termite laboratory bioassay to compare the various preferred termite protocols used by IRG termitologists was initiated. The author was nominated to co-ordinate this comparative laboratory evaluation of two wood preservatives, copper-chrome-arsenic (CCA) and copper naphthenate (Cu-Na) against the subterran...
J R J French


Use of biossay to determine CCF and CCB preservative retentions in treated Pinus sylvestris
1984 - IRG/WP 2216
The retention of each a copper/chromium/boron and copper/chromium/fluoride preservative in pressure treated pinepoles was tested by bioassay using Aspergillus niger as test fungus. Small samples of 5 mm³ were taken from various depth of the poles and placed on potato-dextrose agar plates. The inhibition of fungal growth depends on the preservative retention in the cubes and corresponds closely to...
J C Moreschi, H Willeitner


Laboratory and field evaluation of Plasmite Reticulation System using bifenthrin as a chemical barrier within wall cavities against subterranean termites.
2005 - IRG/WP 05-20307
Laboratory and field bioassays undertaken to demonstrate Plasmite Reticulation system effectively delivers the termiticide (bifenthrin) within a simulated wall cavity at the required concentration. The chemical assay indicated that the amount of bifenthrin sampled at 5, 10, 15, 20, and 25m along the simulated reticulation system tested (30m) exceeded the manufacturer’s minimum recommendation of ...
J R J French, B M Ahmed, J Thorpe, A Anderson


A selective-choice laboratory bioassay technique with Nasutitermes exitiosus (Hill) (Isoptera: Termitidae)
1983 - IRG/WP 1176
This paper presents a laboratory bioassay technique for simultaneous exposure of a variety of specimens to the same termite biomass. Details of the technique are given in a small scale selective-choice assessment with Nasutitermes exitiosus (Hill) in which timber species susceptibility is compared together with the efficacy of sub-lethal concentrations of protective compounds....
C D Howick, J W Creffield, P R Burridge


Correlation between a laboratory bioassay and field trial conducted to determine the termiticidal effectiveness of bifenthrin
2002 - IRG/WP 02-20248
Details are given of a laboratory bioassay and field trial undertaken to determine the termiticidal effectiveness of the synthetic pyrethroid bifenthrin, when impregnated into Pinus radiata D. Don sapwood specimens. Results show a strong correlation between the laboratory and field methods of evaluation. Protection threshold limits obtained were the same for the two test species of termite employe...
J W Creffield, K Watson


Susceptibility of softwood bait stakes to attack by subterranean termites (Isoptera: Rhinotermitidae)
1994 - IRG/WP 94-20037
Sapwood stakes of Australian-grown Araucaria cunninghamii (hoop pine), Pinus elliotii (slash pine), Pinus radiata (radiata pine) and North American-grown Pinus sp. (southern yellow pine) were exposed to subterranean termite attack in an in-ground bioassay. Stakes in bait containers and bare stakes were attacked by Coptotermes acinaciformis and Schedorhinotermes intermedius. Basic susceptibility of...
B C Peters, R T Murray, C J Fitzgerald


Susceptibility testing protocol for powderpost beetles in Australia
2002 - IRG/WP 02-20242
Several species of lyctine (powderpost) beetle are able to attack a range of hardwood timbers in Australia. Powderpost beetles infest only the starch-containing sapwood of certain hardwoods and do not infest softwoods. Attack by powderpost beetles on susceptible timber in Australia is almost inevitable and may continue until the food resource is completely utilised. Prevention of powderpost beetl...
B C Peters, J W Creffield, R H Eldridge


Development of an Australasian protocol for assessment of wood preservatives
1994 - IRG/WP 94-20043
The Australasian Wood Preservation Committee (AWPC) is currently developing a suite of assessment procedures (protocols) for the biocidal efficacy of wood preservatives for approval in Australasia (Australia, New Zealand, Papua New Guinea, Fiji). Protocols are being prepared for the six hazard levels recognised in the relevant standards of member countries and represent the minimum procedures requ...
W D Gardner, H Greaves, M E Hedley, K J McCarthy, J Norton


Evaluating the potential of modified wood for use in marine environments using a short-term laboratory bioassay
2004 - IRG/WP 04-10525
Chemically modified wood may be an alternative to preservative treated timber for marine structures. In this study a screening laboratory test using the wood-boring isopod crustacean Limnoria quadripunctata was used to assess the durability of chemically modified Pinus sylvestris, Pinus radiata and Picea sp. Most of the treatments used a combination of one of two of types of the resin dimethyloldi...
L M S Borges, S M Cragg, M van der Zee


Laboratory bioassay on the termiticidal efficacy of two ACQ formulations
1999 - IRG/WP 99-30199
The termiticidal efficacy of two ammoniacal copper quaternary ammonium formulations (ACQ) was evaluated in a laboratory bioassay using two species of subterranean termites, Mastotermes darwiniensis Froggatt and Coptotermes acinaciformis (Froggatt). Five retentions (1.0, 2.0, 4.0, 6.0 and 8.0 kg/m3 of active ingredient) of each ACQ formulation (MitrexACQ and ACQ97) were assessed in sapwood specimen...
J W Creffield, A F Preston, N Chew


The preventive actions of three commercial wood preservatives against Dinoderus minutus
1984 - IRG/WP 1233
Dinoderus minutus is one of the most common pest insects for the bamboos. For preventing the damages of this insect, the preventive treatment of bamboos with preservatives is necessary. But because of the environmental reasons, only limited insecticides are available in Japan. The author determined the preventive effects of three commercial products against Dinoderus minutus by the medium of the B...
K Suzuki


A laboratory bioassay on the termiticidal efficacy of a chlorothalonil formulation and a chlorothalonil plus chlorpyrifos formulation to Mastotermes darwiniensis Frogatt
1993 - IRG/WP 93-30004
Results of a laboratory bioassay on the efficacy of two preservative formulations (chlorothalonil in oil; chlorothalonil plus chlorpyrifos in oil) to the Australian subterranean termite Mastotermes darwiniensis Froggatt are given. Specimens of Pinus radiata D. Don sapwood were treated to three retentions of each formulation to achieve 3.2, 6.4 and 12.8 kg/m³ of chlorothalonil a.i. and 3.2 + 0.2, ...
J W Creffield, N Chew


Natural durability transfer from sawmill residues of white cypress (Callitris glaucophylla). - Part 2: Laboratory fungal bioassays
2000 - IRG/WP 00-20204
Extracts from sawmill residues of the naturally durable white cypress, Callitris glaucophylla were tested for fungicidal activity in a series of laboratory bioassays. The effects of different extraction solvents, techniques and sources of material on the biocidal efficacy of the resultant extracts were evaluated. Soil jar decay tests were used initially however, contractual time constraints necess...
M A Powell, L M Stephens, L Francis, M J Kennedy


Bioprocessing preservative-treated waste wood
2000 - IRG/WP 00-50145
Disposal of preservative-treated waste wood is a growing problem worldwide. Bioprocessing the treated wood offers one approach to waste management under certain conditions. One goal is to use wood decay fungi to reduce the volume of waste with an easily managed system in a cost-effective manner. Wood decay fungi were obtained from culture collections in the Mycology Center and Biodeterioration res...
B Illman, V W Yang, L Ferge


Laboratory evaluation of borate formulations as wood preservatves to control the subterranean termite coptotermes acinaciformis (isoptera: Rhinotermitidae) in Australia
2001 - IRG/WP 01-30266
The termiticidal efficacy of Borocol (sodium octaborate tetrahydrate), boric acid, bore-ester-7 and tri- methyl borate was evaluated in laboratory bioassays against Coptotermes acinaciformis (Froggatt). Seasoned sapwood blocks of Pinus radiata D. Don, and Eucalyptus regnans (F. Muell) were impregnated with the various borate compounds. There were marked differences in mass loss and mortality rate ...
B M Ahmed, J R J French, P Vinden


Chemical evaluation of borate treated pine sapwood attacked by the subterranean termite Coptotermes acinaciformis
1993 - IRG/WP 93-20003
Sapwood of hoop (Araucaria cunninghamii Ait. ex D. Don) and slash (Pinus elliottii Englem.) pines were treated by Vacuum Pressure Impregnation (VPI) to provide test specimens. The concentration of boron was defined by chemical analyses of the timber, in the zone of primary attack by the termite being bioassayed. A common method of definition of preservative retention, weight uptake of preservative...
A R Moffat, B C Peters


Establishing standard principles for laboratory bioassays of termiticides with subterranean termites - progress, problems and prospects
1993 - IRG/WP 93-10013
Laboratory bioassays of termiticides, including wood preservatives, aim to give an indication of the likely concentrations effective in preventing damage to timber products and other materials in the field. In laboratory bioassays field conditions should be simulated as closely as possible. With a wide range of procedures in use around the world it may often be difficult to compare results between...
M Lenz, J W Creffield, Zhong Yun-hong, L R Miller


Estimation of oral toxicity of boron as a bait toxicant and the trophallactic effects between individual members of termite colonies.
2003 - IRG/WP 03-10495
In recent years, because of the favourable environmental characteristics of boron, researchers in the wood preservation industries have refocussed on the use of boron as a major wood preservative against wood-destroying insects. Currently the greatest use of boron compounds is in remedial treatments. Boron has been found to have slow-acting toxicity against subterranean termites. Because of thi...
B M Ahmed


Protection of southern pine using N,N-Napthaloylhydroxylamine: Field tests, soft-rot cellars and aquatic bioassay leach testing
1999 - IRG/WP 99-30204
Recent environmental restrictions are limiting the use of broad-spectrum biocides for wood preservation. There is an urgent need for new, sharply targeted, environmentally benign wood preservatives. N'N-Napthaloylhydroxylamine (NHA), a water-soluble calcium-precipitating agent, has been shown to inhibit decay by brown-rot and white-rot fungi in soil-block tests and prevent damage by Easte...
D M Crawford, F Green III


International collaborative laboratory comparison of two wood preservatives against subterranean termites: Update
1994 - IRG/WP 94-20032
It was agreed by members at the termite workshop at the 24th annual meeting of IRG in Orlando, USA, in May 1993 to initiate an international subterranean termite laboratory bioassay to compare the various preferred termite protocols used by IRG termitologists. The author was nominated to co-ordinate this comparative laboratory evaluation of two wood preservatives (CCA and Cu-naphthenate) against t...
J R J French


Toxic value estimation of wood preservatives by using the probit analysis
1990 - IRG/WP 2348
As it was assumed from previous experiments, the probit analysis appears to be the most suitable method of all used to estimate the toxic value of wood preservatives. With the help of the classical agar-block method the probit analysis was tested for its suitability in: 1) fungitoxic value estimation of the WR-3 preservative (quaternary ammonium compound + borate), as compared to different test fu...
J Wazny, K J Krajewski


The metabolism and comparative elimination of polychlorinated biphenyl congeners in termites
1993 - IRG/WP 93-10038
Termites may serve as a potential supplementary food source for fish, poultry and pigs. Waste paper may be used as a source of food in mass rearing the termites. However, paper products and printing inks contain trace levels of toxic xenobiotics e.g. polychlorinatecl biphenyls (PCBs) and dioxins. This study examined the ability of Mastotermes darwinensis and Coptotermes acinaciformis to metabolise...
V S Haritos, J R J French, J T Ahokas


Laboratory evaluation of the termiticidal effectiveness of TanalithÒ 3485
1995 - IRG/WP 95-10109
The termiticidal effectiveness of the copper azole TANALITH 3485 was evaluated with the benchmark preservative TANALITH C in a laboratory bioassay using two species of subterranean termites, Mastotermes darwiniensis Froggatt and Coptotermes acinaciformis (Froggatt). Retentions of TANALITH 3485 tested were 0.15, 0.24, 0.285 and 0.40% m/m Cu and for TANALITH C 0.025, 0.05, 0.08 and 0.095% m/m Cu. Un...
J W Creffield, J A Drysdale, N Chew, N-K Nguyen


A bioassay for appraising preservative protection of wood above ground
1978 - IRG/WP 2124
A bioassay, using the mold fungus Aspergillus niger, gave results that were correlated with amounts of pentachlorophenol and tributyltin oxide in pine sapwood treated with solutions ranging in strength from 0.016 or below to 5%. Limited bioassay estimates of penta in commercially treated millwork corresponded to estimates by lime ignition. The assay fungus exhibited somewhat greater tolerance of t...
T C Scheffer, L Gollob


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