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Durability against fungal decay of sorbitol and citric acid (SorCA) modified wood
2022 - IRG/WP 22-40928
Most European-grown wood species are susceptible to biological degradation, specifically, they suffer from a poor resistance against wood-destroying fungi. Therefore, prior to outdoor exposure, wood has to be treated either by applying a protective coating on its surface or by full-volume impregnation with antifungal chemicals. However, due to environmental and health concerns, the most frequently...
K Kurkowiak, L Emmerich, H Militz


A novel cellulose-binding domain from the brown-rot fungus Gloeophyllum trabeum
2023 - IRG/WP 23-11019
Wood-rotting basidiomycetes are the major organisms decomposing wood in nature. They are classified into two groups based on their decay modes; white-rot fungi and brown-rot fungi. White-rot fungi secrete various cellulolytic enzymes during the wood degradation process. The enzymes are known to be often appended with a cellulose binding domain (CBD) which assists the activity of catalytic domain. ...
Y Kojima, N Sunagawa, M Aoki, M Wada, K Igarashi, M Yoshida


Morphological observation of wood at the early stages of decay in brown rot and white rot
2023 - IRG/WP 23-11020
Wood rotting fungi, the fungal species causing biodeterioration for wood building, are generally classified into white-rot, brown-rot and soft-rot fungi based on their decay modes. Since white-rot and brown-rot fungi are known to reduce wood strength significantly, it is important to clarify the mechanisms of their wood degradation. White-rot fungi reduce wood strength as the decay progress and de...
R Tsukida, T Hatano, Y Kojima, Y Horikawa, S Nakaba, R Funada, M Yoshida


The cellulose binding mechanism of a novel cellulose binding domain from the brown-rot fungus Gloeophyllum trabeum
2023 - IRG/WP 23-11021
In nature, wood decay is caused by various wood-rotting basidiomycetes. Wood-rotting basidiomycete are mainly divided into white-rot fungi and brown-rot fungi. Their main carbon source is cellulose of the wood cell wall during wood decay, and they produce a variety of enzymes to decompose cellulose. The cellulolytic enzymes often possess a cellulose binding domain (CBD) as an additional domain con...
M Aoki, Y Kojima, M Wada, M Yoshida


Research on gaseous COS degradation by brown-rot fungus Gloeophyllum trabeum
2024 - IRG/WP 24-11033
Physiological studies of wood rotting fungi have mostly focused on the metabolism of carbon and nitrogen sources, which constitute the dominants components of wood. On the other hand, despite the physiological importance of trace elements such as sulfur, studies on their acquisition sources and metabolic pathways are limited. Until now, wood rotting fungi have been thought to utilize slight amount...
R Iizuka, R Tsukida, Y Katayama, M Yoshida


A novel cellulose-binding domain from the brown-rot fungus that can be used to evaluate cellulose in wood
2024 - IRG/WP 24-11046
Wood-rotting basidiomycetes are the primary microorganisms that decay wood in nature. They are classified as white-rot fungi and brown-rot fungi by the difference in decaying types. White-rot fungi secrete a variety of cellulolytic enzymes during wood degradation. These enzymes often have an additional cellulose-binding domain (CBD) that adsorbs to the cellulose surface and localizes the catalytic...
Y Kojima, N Sunagawa, S Tagawa, T Hatano, S Nakaba, M Aoki, M Wada, K Igarashi, M Yoshida


Enhancing UV resistance and decay resistance of wood by nanoparticle dispersed linseed oil nanoemulsion
2024 - IRG/WP 24-20706
Enhancing increasing demand for eco-friendly and cost-effective wood coatings and preservatives has led to the development of water-borne formulations and use of non-toxic and natural materials such as vegetable oils and waxes. The aim of this study was to improve the UV resistance and decay resistance of wood by nanomaterial dispersed linseed oil emulsions. Highly stable nanoemulsions of linseed ...
R Bansal, R Kumar, K K Pandey


Synthetic Oxalate/ß-glucan Fungal Extracellular Matrix Demonstrates Potential Inhibition of Extracellular Enzyme Diffusion into Wood Cell Walls, and Calls into Question the Role of Enzymes in Wood Decay
2025 - IRG/WP 25-11053
ß-glucan is the major component of the extracellular matrix (ECM) of many fungi, including wood degrading fungi. Many of these species also secrete oxalate into the ECM. Our research demonstrates that ß-glucan forms a novel, previously unreported, hydrogel at room temperature with oxalate. This finding better explains the gel-like nature of the fungal ECM. Oxalate, at relatively low levels, was ...
B Goodell, G A Tompsett, G Perez-Gonzalez, K Mastalerz, M Timko


Distribution of Crystalline Cellulose-Binding Domain CBM104 in Wood Rotting Fungi
2025 - IRG/WP 25-11073
Wood rotting fungi play a crucial role in biodeterioration of wood. Many brown-rot fungi are known to rapidly degrade cellulose in wood despite lacking enzymes with cellulose-binding domains. This has led to the hypothesis that they rely on a non-enzymatic degradation system. We recently discovered a novel cellulose-binding domain, CBM104, in the brown-rot fungus Gloeophyllum trabeum. In this stud...
Y Kojima, N Sunagawa, M Aoki, S Tagawa, M Wada, K Igarashi, M Yoshida


Investigation of oxalic acid production in brown-rot fungi
2025 - IRG/WP 25-11074
Brown-rot fungi play a crucial role as wood decomposers in forests and are also known as the main cause of biodeterioration of wooden buildings in Japan. Therefore, understanding their decomposition mechanisms is important for the maintenance and preservation of wooden structures. While white-rot fungi generally use enzyme-system for degradation, many brown-rot fungi lack cellobiohydrolases which ...
S Matsumoto, R Tsukida, T Ito, M Iritani, M Yoshida


Effect of the Cellulose-Binding Domain Associated with Xylanase on the Degradation of Softwood and Hardwood Xylan
2025 - IRG/WP 25-11078
Brown rot fungi rapidly degrade hemicellulose, which is recognized as a key decomposition process during the early stages of wood decay. Brown rot fungi possess multiple genes encoding hemicellulose-degrading enzymes, suggesting that enzymatic hydrolysis plays an important role in this process. Hemicellulases produced by these fungi often contain an additional domain classified as carbohydrate-bin...
R Tsukida, Y Kojima, S Kaneko, M Yoshida


Synergistic effect of the association of Prosopis juliflora polyphenolic extractives with tebuconazole on the growth inhibition of brown and white rot fungi: a solution to increase the naturality and safety of wood preservation treatment
2025 - IRG/WP 25-20732
The antifungal effect of catechin and extractives from Prosopis juliflora was studied against one white rot fungus, Trametes versicolor (TV), and one brown rot fungus, Rhodonia placenta (PP). The extractives from Prosopis julilfora were crude mesquitol and pure mesquitol. Tebuconazole was used in this study as a known fungicide against the two named fungi. Wood protection using fungicides can be h...
J Owino, J Tuimising, F Mangin, P Gérardin, A Kiprop, C Gérardin-Charbonnier


Effect of photodegradation on fungal colonization on wood during initial stage of brown-rot decay
2025 - IRG/WP 25-41024
Photodegradation and decay may happen simultaneously on wood under many exposure conditions. The aim of this study was to explore the effect of photodegradation on fungal colonization during initial stage of brown-rot decay of wood. For this purpose, southern pine (Pinus spp.) wood was exposed to accelerated UV weathering for different durations, and then decayed by a brown rot fungus (Gloeophyllu...
Y Wang, Y Peng, J Cao


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