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Method of embedding and staining of wood after biological testing to support the identification of decay type
1998 - IRG/WP 98-20131
A method for preparing wood for microscopic analysis after decay testing is described, with special regard to a novel fast simultaneous staining, which helps to identify different types of decay. The novel staining process is explained as well as the embedding of heavily decayed samples in polyethylene glycol (PEG) and picking up of sections by transparent adhesive tape. Special feature of the int...
A O Rapp


Minimum inhibitory concentration of methylene bisthiocyanate towards selected wood inhabiting fungi in liquid culture and on radiata pine
2001 - IRG/WP 01-30261
The objective of the current study was to determine the minimum inhibitory concentration (MIC) of methylene bisthiocyanate (MBT) towards wood inhabiting fungi common on unseasoned radiata pine (Pinus radiata D. Don). The fungi used were Ophiostoma floccosum, Leptographium procerum, Sphaeropsis sapinea and Trichoderma viride. Fungi were individually inoculated into nutrient media spiked with differ...
T Singh, B Kreber, C Chittenden, R N Wakeling, A Stewart


Laser incising of spruce lumber for improved preservative penetration
1991 - IRG/WP 3646
Red spruce heartwood specimens were incised using a range of laser beam power levels and pulse durations. The penetration of CCA through these holes into the wood was monitored after pressure treatment. In addition, green and dried spruce samples were incised using two patterns with incision densities of either 16.1 holes/in² or 21.3 holes/in². Following CCA pressure treatment the samples were t...
B Goodell, F A Kamke, Jing Liu


Immuno-scanning electron microscopic localization of extracellular polysaccharidases within the fibrillar sheath of the brown rot fungus Postia placenta
1991 - IRG/WP 1497
Extracellular polysaccharidases of the brown-rot fungus Postia placenta were localized using colloidal gold labeled monoclonal antibodies to the B-1,4-xylanase (32-36kDa) fraction of Postia placenta. Postia placenta was grown from agar onto glass coverslips, immunolabeled with or without prior fixation, and examined by SEM. Enzymes were localized on the hyphal surface and on the clumped fibrillar ...
F Green III, C A Clausen, M J Larsen, T L Highley


A comparison of analytical and visual techniques used for assessment of weathering properties of chromium and copper azole treated timber
1994 - IRG/WP 94-20023
The weathering properties of preservative treated timber were studied by measuring surface lignin loss and by microscopic examination after weathering, and results were related to qualitative assessments of visual appearance after weathering. Measurement of relative rates of delignification of untreated and preservative treated timber obtained by FTIR analysis, and weight loss determined on thin v...
J A Cornfield, M D C Hale, G Fettis


Preliminary Studies on ZiBOC- A Potential Eco-friendly Wood Preservative
2005 - IRG/WP 05-30372
Over the past decade, the wood processing industry has been increasingly involved in strategies to minimize the environmental impacts of treated wood. One sign of this is the dramatic increase in the use of preservatives based on inorganic metal oxides, which fix in wood. Among such preservatives the most common is chromated-copper-arsenate. In Ascu chromium and arsenic both are carcinogenic thus...
S Tripathi, J K Bagga, V K Jain


Evaluation of rapid methods for detecting wood preservatives in waste wood
1994 - IRG/WP 94-50024
For the disposal of wood waste under ecological sound conditions information about its hazardous potential is required. Several analytical methods are available for the detection of most elements in wood preservatives. Industrial process conditions, however, demand methods with very short turn around times. In this research several methods of analysis were judged on their speed and accuracy. A num...
W J Homan, H Militz


Hole delimination inside round timber via ultrasonic techniques
1990 - IRG/WP 2358
A fast and economic circular scanning method to localize and evaluate holes inside round timber is presented. This method consists in measuring transit times of the ultrasonic wave by fixing the transmitter probe in the perimeter of the sample, then moving the receiver probe every ten degrees until completion of the circle. The scanning is done with a cheap and light instrument. Tests are made wit...
G Prieto, A Fernández Cancio


Comparative study between full cell and passive impregnation method of wood preservation for laser incised Douglas-fir lumber
2007 - IRG/WP 07-40359
Many wood species are highly resistant to conventional pressure treatment. To improve the chemical impregnation into Douglas-fir (Pseudotsuga menziesii Franco) lumber, the passive impregnation method had been developed. In this study, ability of the passive impregnation for lumber was compared with a conventional full cell method. Square Douglas-fir lumber with the incising density of 10,000 holes...
Md N Islam, K Ando, H Yamauchi, Y Kobayashi, N Hattori


Laser stain removal of fungus-induced stains on paper-based cultural relics
2007 - IRG/WP 07-40389
Laser techniques has been developed and applied in commercial use for 40 years, but it has not been used successfully to remove stains from paper-based cultural relics . The purpose of this study was to investigate the paper cleaning by using a technique of Nd-YAG laser. Artificial stained papers were inoculated with fungi and then were treated at different laser cleaning parameters, in order to f...
Tsang-Chyi Shiah, Han Chien Lin


Effects of Moisture Content and Species on Penetration of Liquid in Laser Incised Lumber by the Passive Impregnation Method
2008 - IRG/WP 08-40408
Penetration of liquid by passive impregnation method for Douglas-fir (Pseudotsuga menziesii Franco), sugi (Cryptomeria japonica D. Don) and Japanese larch (Larix leptolepis Gordon) were studied. Effects of initial moisture content of sugi (16, 43 and 70% moisture content) on liquid penetration by the method were also studied. Dried Douglas-fir and sugi, and green Japanese larch and sugi were treat...
N Hattori, M Nazrul Islam, K Ando, H Yamauchi


Natural durability and anatomical features of teak (Tectona grandis) from plantations in Costa Rica
2008 - IRG/WP 08-10671
Teak (Tectona grandis) possesses superior mechanical properties and durability and is thus frequently used as an alternative to impregnated timber in Europe. Demand on teak has resulted in severe exploitation of the tropical teak forests in recent decades, but today the imported timber originates mainly from plantations and is certificated according to the Forest Stewardship Council (FSC) policy. ...
U J Wolfsmayr, N Terziev, G Daniel


Durability and fungal colonisation patterns in wood samples after six years in soil contact evaluated with qPCR, microscopy, TGA, chitin- and ergosterol assays
2009 - IRG/WP 09-20402
There is a need to establish new objective and sensitive methods for early detection and quantification of decay fungi in wood materials. Molecular methods have proven to be a useful tool within wood protection issues, however, this field is still poorly explored and so far relatively few have used these methods within the field of wood deterioration. Among the techniques used in the indirect quan...
A Pilgård, G Alfredsen, I Børja, C Björdal


Use of Confocal Profilometry to Quantify the Weathering of Wood
2009 - IRG/WP 09-20421
The methods that have been used to screen chemicals for their ability to prevent the weathering of wood involve assessing the loss in weight and tensile strength of treated wood veneers or measuring the rate of erosion of exposed and unexposed (masked) wood subjected to weathering. The erosion of wood during natural weathering occurs slowly and long exposure times are required to produce differenc...
Chunling Liu, P Evans


Weathering of Wood Modified with the N-Methylol Compound 1,3‑dimethylol-4,5-dihydroxyethyleneurea (DMDHEU)
2009 - IRG/WP 09-40467
Scots pine (Pinus sylvestris L.) sapwood boards were treated with a methylated 1,3‑dimethylol-4,5-dihydroxyethyleneurea (mDMDHEU) causing weight gains of approx. 25% and exposed to weathering for 18 months. Compared to untreated boards, treatment with mDMDHEU reduced surface discoloration mainly caused by staining fungi. Boards treated with mDMDHEU exhibited clearly lower moisture content th...
C Mai, Yanjun Xie, A Krause, K Urban, P D Evans, K Richter, H Militz


The invasion channels of damage fungi in bamboo lumber
2010 - IRG/WP 10-10712
The micro-agents on bamboo lumber damage are mould, stain, and decay fungi. This research is about invasion channels of bamboo lumber damage fungi by scanning electron microscope (SEM). The results showed that the conidia of mould were only accumulated on the surface of bamboo and no hyphae detected in bamboo vascular bundle, and hyphae of stain penetrated into bamboo and grew in metaxylem vessel...
Xingxia Ma, Mingliang Jiang, Daochun Qin


Microscopy evaluation of microbial decay patterns in wood stakes after 6 years in soil contact
2010 - IRG/WP 10-10715
Most studies on wood modification have so far been concerned with service life predictions rather than investigating the decay protection mechanism. Very little is known about the mode of action of these new treatments and about the strategies used by wood degraders in order to utilize the wood material despite a treatment. Access to such knowledge would allow further modification and improvement ...
A Pilgård, C G Björdal


The Use of Micro-Tensile Testing to Assess Weathering Decay and Oxidative Degradation of Wooden Items
2010 - IRG/WP 10-20433
This paper presents a method to study the weathering performance of wooden items and to assess the oxidative degradation of wood via the Fenton reaction. Weathering resistance and photo-stability of wood was tested using pine wood (Pinus sylvestris) veneer strips measuring approximately 60 µm in thickness. The veneer strips were treated using a reactive chemical to impart surface protection. The ...
C Mai, Yanjun Xie, Zefang Xiao, P D Evans, H Militz


Using plant oils as hydrophobic substances for wood protection
2010 - IRG/WP 10-30550
The increased interest to use oils as hydrophobic agents and the current debate about the further use of creosote emphasises the urgent need of better understanding of plant and other oils as wood protectors. The present study sheds light on the hydrophobic properties and distribution of various plant oils in the anatomical structure of Scots pine sapwood. Oil retentions from approximately 70 to 5...
D Panov, N Terziev, G Daniel


Slice - Cut and Analyze Laser Microdissection (LMD): A method to produce sample material from modified wood for chemical quality analysis
2011 - IRG/WP 11-20478
Wood modification with its various systems is an established way to enhance a wood products’ service life in outdoor applications. Per definition, selected material properties are enhanced by means of modification without producing toxic substances or residues during service life and combustion afterwards. The systems of impregnation modification include a physical and often chemical alteration ...
K Zimmer, E Larnøy, A Treu, M Fongen


Copper Nanoparticles in Southern Pine Wood Treated with a Micronised Preservative: Nanodistribution of Copper in the Pit Membrane and Border of an Earlywood Bordered Pit
2011 - IRG/WP 11-30566
Copper nanoparticles can penetrate the cell walls of unlignified parenchyma cells in southern pine wood treated with a micronised wood preservative, but they are excluded from lignified tracheid walls. This paper extends these observations to include the cell wall layers of the bordered pit. Focused ion beam and ion milling were used to make an ultra-thin section of the cell wall layers of an earl...
H Matsunaga, Y Kataoka, M Kiguchi, P Evans


Further Studies on the Distribution of Copper in Treated Wood Using an XRF Microscope Technique
2011 - IRG/WP 11-40549
There are two distinct forms of copper in aqueous copper based wood preservative systems that are currently used in the United States for treatment of southern pine lumber. These are systems using either soluble copper (typically amine based) or the more recent particulate or “micronized” copper as the primary biocide. Studies reported last year showed that there were distinct differences in...
A Zahora


Novel microscopic approaches to visualise chitosan within impregnated wood
2012 - IRG/WP 12-20485
Chitosan, a deacetylated product of an abundant naturally occurring biopolymer chitin, has been used in a range of applications, particularly in food and health areas, as an antimicrobial agent. In the work reported here Pinus radiata wood was impregnated with chitosan as an environmentally compatible organic biocide. It is important to understand micro-distribution of bioprotectants in impregnate...
A Singh, T Singh


Evaluation of fungal infestation and decay in a simulated use class 3 situation (block test) after some years of exposure
2012 - IRG/WP 12-20487
The so named “block test” was designed as part of the assessment methodology for testing the behaviour of natural and modified wood used under use class 3 (EN 335-2) conditions. The test was developed to expose the wood close to the ground to an environment with high humidity and high biological activity, but not in soil contact. The present study describes the evaluation of fungal infestatio...
A Gellerich, K Röhl, S Adamopoulos, H Militz


Accessibility of Wood Cell Walls to Well-defined Platinum Nanoparticles
2012 - IRG/WP 12-20494
Copper nanoparticles are found in the walls of parenchyma cells in southern pine sapwood treated with a micronised wood preservative, but they are absent from tracheid walls. Hence, we hypothesized that small nanoparticles can penetrate the walls of unlignified parenchyma cells, but are excluded from lignified tracheid walls. This paper tests this hypothesis by treating pine sapwood with an aqueou...
H Matsunaga, Y Kataoka, M Kiguchi, P D Evans


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