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The effects of acetylation level on the growth of Postia placenta
2011 - IRG/WP 11-10751
To understand the defence mechanisms utilized by decay fungi when exposed to different wood protection systems the study of gene expression can give us some answers. When the DNA sequences are known, primers can be designed to detect transcripts of genes with gene products related to basic cellular processes and hyphal growth. The characteristic gene products induced in different fungi by differen...
A Pilgård, G Alfredsen, C G Fossdal, C J Long II


Variation in two Postia placenta strains, MAD-698-R and FPRL 280 – mass loss, DNA content and gene
2012 - IRG/WP 12-10781
Brown-rot fungi such as Postia placenta are common inhabitants of forest ecosystems and brown rot fungi are also largely responsible for the destructive decay of wooden structures. The aim of this study was to compare two commonly used strains of Postia placenta – MAD-698-R and FPRL 280. Scots pine sapwood samples were exposed for two and eight weeks to both fungal strains. The following was inv...
N Thaler, G Alfredsen, C G Fossdal


Analyses of premature failure of utility poles
2012 - IRG/WP 12-40584
In this study a total number of 18 utility poles of Scots pine (Pinus sylvestris) impregnated with a copper-chromium containing preservative were investigated. They were part of different lower voltage transmission lines in the western part of Germany and failed before predicted minimum service life. All poles in this study were less than 15 years in use. The type of decay and fungi were evaluated...
S Bollmus, N Rangno, H Militz, A Gellerich


The effects of acetylation level on the growth of Postia placenta over 36 weeks
2012 - IRG/WP 12-40589
Genomic sequencing gives us a tool to systematically and rapidly discover novel genes, how their products function in the cell, and explore their interactions. When the DNA sequences are known, primers can be designed to detect transcripts of genes with gene products related to basic cellular processes and hyphal growth. The characteristic gene products induced in different fungi by different wood...
A Pilgård, G Alfredsen, C G Fossdal, C J Long II


High-throughput sequencing highlighted contrasted pioneer fungal communities associated to coniferous and deciduous wood preservation assays
2013 - IRG/WP 13-10800
Studying the fungal communities in the wood, in particular during the first events of the colonization, and the factors that underlie the dynamics of fungal species assemblages remain a challenge in ecology, because of the absence of fructification during the pioneer steps of wood degradation. The use of Next-generation DNA sequencing methods, which produce massive volumes of data, provided new pe...
Y Mathieu, A Dassé, I Le Bayon, M Kutnik, L Harvengt, E Gelhaye, M Buée


A case study of investigating fungi that affect traditional Japanese shake roof with/without copper plates
2013 - IRG/WP 13-10810
A Japanese traditional shake roof is made from split logs. To keep the roof long, copper (Cu) plates are often inserted between the shakes. It has been thought that Cu elements from copper plates would flow out when a shake roof is exposed to rain and inhibit growth of wood rotting fungi. Actually there are some examples where the Cu plates seem to be effective in preventing fungal damage, but on ...
T Wada, Y Fujiwara, Y Fujii, R Kigawa


Extraction and analysis of DNA from green and seasoned timber as basic methods for determination of wood species and origin
2013 - IRG/WP 13-20523
Against the background of the European timber trade regulation EUTR, commenced to law by March 2013, the determination of wood species and tracing of its origin is getting a great importance. A promising approach for establishing fast and reliable tracking systems for wood products is DNA analysis. A critical point is the extraction of analysable DNA from the wood and its lignified cell walls. Thu...
K Jacobs, H Mende, W Scheiding


What molecular biology can tell us about the biodegradation of lignocellulose: the utilization of molecular techniques for the detection, identification and enhanced understanding of wood degrading organisms
2013 - IRG/WP 13-20528
Molecular techniques are now routinely used in the identification, detection and analysis of wood degrading organisms. An overview of some of the early work on nucleic acid isolation and characterization will be followed by a discussion of the power of sequencing and other procedures for better understanding: the mechanisms involved in the biological degradation of wood, the metabolic basis of pre...
J Jellison, B Goodell, G Alfredsen, D Eastwood, G Daniel, S M Cragg, J K Grace


Molecular characterization and biodiversity of wood-decaying fungi in French Guiana
2014 - IRG/WP 14-10825
Fungi from tropical regions are currently under-represented in the classification system. Indeed, difficult access to tropical forests and irregular occurrence carpophores make it complicated to study fungus species in such environments, unlike in European zones where fungal diversity and taxonomy are better known. The purpose of this work was to enhance classification by integrating new data that...
A Zaremski, L Gastonguay, C Zaremski, J Beauchene


Microbial Community Analysis of Naturally Durable Wood in an Above Ground Field Test
2014 - IRG/WP 14-10826
This paper presents preliminary results of an above ground field test wherein eight naturally durable wood species were exposed concurrently at two sites in North America. Surface samples were taken at regular intervals from non-durable controls and compared to their more durable counterparts. Terminal Restriction Fragment Length Polymorphism was performed to characterize the microbial (bacteria, ...
G T Kirker, S V Diehl, P K Lebow


Genetic characterisation of the deathwatch beetle – Call for support
2017 - IRG/WP 17-10890
The deathwatch beetle (Xestobium rufovillosum) is one of the most important pests of historical wood constructions. Further analysis of the exact nature of the feeding habits regarding diet and wood characteristics might deliver answers for controlling this pest. Also there is little known about genetic variability and population structure within the species. For analysis of infested wood and also...
M Höpken, E Melcher, J Trautner


Imports of African logs, do they harbor wood-decaying alien fungi and bacteria that could become a potential threat at temperate latitudes? – First results
2018 - IRG/WP 18-10904
In this project we investigate the potential threat of alien wood-decaying fungal and bacterial species imported to Germany with logs from African countries. In a first approach we analysed fungal species on imported logs by identification of fruit bodies by DNA sequencing of the internal transcribed spacer (ITS) regions ITS1 and ITS2. First results revealed the existence of at least one alien fun...
J Trautner, M Höpken, E Melcher


Biodiversity of wood-decaying fungi in French Guiana sequences of the small subunit (SSU) of ribosomal (r) DNA and the two primer pairs SR6/SR10R and SR7/SR1R
2019 - IRG/WP 19-10936
Fungi from tropical regions are currently under-represented in the classification system. Indeed, difficult access to tropical forests makes it complicated to study fungus species in such environments, unlike in European zones where fungal diversity and taxonomy are much better known. The purposes of this work were: a) to enhance classification by integrating new data that would bring out the impo...
C Zaremski, A Ducousso-Detrez, N Amusant, A Zaremski


Identifying the fungal community on western redcedar (Thuja plicata) wood in field tests above and in ground contact exposure: preliminary results
2019 - IRG/WP 19-10939
Western redcedar (Thuja plicata) is a high value species in the Canadian forest industry due in large part to the natural durability of its heartwood. Western redcedar heartwood contains extractives that are inhibitory to the growth of many fungi responsible for decay. In order to gain insights into which extractives are important in long term durability, and which fungi are important to decay of ...
A Dale, S Kus, R Stirling


Genetic relationships of local infestations by Anobium punctatum, Xestobium rufovillosum and their associated predator Korynetes caeruleus from buildings in North-Eastern Germany
2021 - IRG/WP 21-10982
Wood-destroying pests such as Anobium punctatum and Xestobium rufovillosum cause damage to art and cultural objects as well as to buildings. Monitoring population dynamics of pest species as well as of their naturally occurring counterparts are an essential part in the development of biological control measures as alternatives to conventional wood protection. Therefore, both the dispersal and homo...
C von Laar, C Baar, R Plarre, D P McMahon


Role of microbiota in wood degradation by Reticulitermes grassei and Bursaphelenchus xylophilus
2022 - IRG/WP 22-20684
Xylophagous organisms can cause damage both in forests and in felled wood. Bursaphelenchus xylophilus, which causes "Sudden Pine Wilting" in coniferous forest masses, and is currently considered a quarantine organism in the European Union. On the other hand, structural and carpentry wood is affected by subterranean termites (Reticulitermes spp.), that cause serious damage, both in buildings and fu...
L Robertson, S Rames, M Uriel, J M González, F Llinares, S M Santos, M T Troya


Characterization of the fungal communities associated with Aquilaria crassna Pierre ex Lecomte plantations in French Guiana
2023 - IRG/WP 23-11001
Aquilariais a tree species belonging to the family of the Thymeleaceae. When the tree is wounded, it can produce a blackened wood, also known as agarwood. The blackened wood has a darker colour than healthy wood and gives off a strong fragrance greatly appreciated by perfumers and by certain oriental religious communities. Production of this blackened wood is deemed to depend on environmental fact...
C Zaremski, C Malandain, O Sibourg, B Heuclin, N Amusant, M Ducousso, A Zaremski


Isolation of the microbiota from wood decay insects and some of their enzymatic activities
2023 - IRG/WP 23-11014
Wood decay organisms have the ability to degrade wood components (cellulose, hemicellulose and lignin) by enzymatic capacities, but these enzymes can belong to the proper organism, or to the associated microbiota. The identification of the microorganisms, as well as their role in the digestion of many substrates not always is very well known. The wood decay insects produce big damages in wood str...
M T Troya, F Llinares, J Arranz, S Izpura-Luis, S M Santos, L Robertson


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