Your search resulted in 37 documents. Displaying 25 entries per page.
Use of the enzyme-linked immunoassay (ELISA) and immunocytochemistry to detect soft rot in preservative treated and untreated wood
1990 - IRG/WP 2347
A polyclonal antibody was raised against mycelial extracts from the soft rot fungus Phialophora mutabilis. In ELISA assays, the antibody showed strong reactions with its own antigen and strong to moderate cross reactions with 6 other Phialophora soft rot species. With the exception of Ceratocystis albida, the antibody gave only weak or negative reactions with 11 other mold, blue stain and rot fung...
G F Daniel, T Nilsson
Effect of Heavy Metals on the Expression of Manganese Dependent Peroxidases of Phanerochaete chrysosporium
2010 - IRG/WP 10-10722
This work is part of an ongoing investigation that studies the application of a solution rich in Zn and Mn, obtained from a recycling process, as a preservative for wood. The effects of Zn2+ and Mn2+ on the enzyme-expression level of manganese-dependent peroxidase (MnP) from Phanerochaete chrysosporium was studied by real-time PCR. The glyceraldehyde 3-phosphate dehydrogenase gene (gpd) was used a...
C Ibáñez, M Rabinovich, M Barraco, G Gecchetto, M Cerdeiras
Structural changes, basidiomycete richness, enzyme activity and proteomic profiling of decay resistant and non-resistant woods over 18 months in soil contact
2010 - IRG/WP 10-10733
Wood decay fungi damage wood by production of enzymes that attack the structural components. The objective of this study was to better understand which suite of decay genes and proteins are expressed during biodeterioration of three different wood types in forest soil over time. Variation in decay genes and proteins were determined for pine (non-resistant), cedar (naturally durable), and ACQ-treat...
Youngmin Kang, S Diehl, L Prewitt, D Nicholas
Measuring fungal cellulolytic enzyme activity in degrading wood
2011 - IRG/WP 11-10753
An enzyme assay which measures enzyme activity directly from wood will assist in the fundamental understanding of the enzyme components of the decay mechanism of rot fungi and any changes in the presence of wood protectants. At present, to measure fungal carbohydrate degrading enzyme activity in the presence of wood, two methods are used: either 1) the enzyme is measured in the growth media contai...
S M Duncan, J S Schilling
New perspectives for wood protection: enzyme-based treatments
2016 - IRG/WP 16-40742
Wood protection research is continuously looking for more benign and effective treatments. One of the main concerns deals with the toxicity of the active compounds employed in the formulations of the preservatives, since they could become a health and environmental issue. Secondly, long-lasting treatments are preferred in order to increase the service life of the treated wood. Weathering condition...
C Fernández-Costas, S Palanti, M Á Sanromán, D Moldes
Development of Potentiometric Enzyme Biosensor for Detection of Permethrin Preservative in Treated Wood
2017 - IRG/WP 17-20628
Characteristics of a potentiometric enzymatic biosensor based on a pH transducer for permethrin detection in treated wood are reported in this article. The transducer contained a plasticiser-free methacrylic-acrylic membrane immobilised with hydrogen ionophore and a lipophilic salt. A poly(2-hydroxyethyl methacrylate) (pHEMA) was used to immobilise carboxylesterase for the detection of permethrin....
M Nasir Mat Arip, C Tan, Gan Kee Seng
Role of microbiota in wood degradation by Reticulitermes grassei and Bursaphelenchus xylophilus
2022 - IRG/WP 22-20684
Xylophagous organisms can cause damage both in forests and in felled wood. Bursaphelenchus xylophilus, which causes "Sudden Pine Wilting" in coniferous forest masses, and is currently considered a quarantine organism in the European Union. On the other hand, structural and carpentry wood is affected by subterranean termites (Reticulitermes spp.), that cause serious damage, both in buildings and fu...
L Robertson, S Rames, M Uriel, J M González, F Llinares, S M Santos, M T Troya
Isolation of the microbiota from wood decay insects and some of their enzymatic activities
2023 - IRG/WP 23-11014
Wood decay organisms have the ability to degrade wood components (cellulose, hemicellulose and lignin) by enzymatic capacities, but these enzymes can belong to the proper organism, or to the associated microbiota. The identification of the microorganisms, as well as their role in the digestion of many substrates not always is very well known.
The wood decay insects produce big damages in wood str...
M T Troya, F Llinares, J Arranz, S Izpura-Luis, S M Santos, L Robertson
Synthetic Oxalate/ß-glucan Fungal Extracellular Matrix Demonstrates Potential Inhibition of Extracellular Enzyme Diffusion into Wood Cell Walls, and Calls into Question the Role of Enzymes in Wood Decay
2025 - IRG/WP 25-11053
ß-glucan is the major component of the extracellular matrix (ECM) of many fungi, including wood degrading fungi. Many of these species also secrete oxalate into the ECM. Our research demonstrates that ß-glucan forms a novel, previously unreported, hydrogel at room temperature with oxalate. This finding better explains the gel-like nature of the fungal ECM. Oxalate, at relatively low levels, was ...
B Goodell, G A Tompsett, G Perez-Gonzalez, K Mastalerz, M Timko
Enzyme activities of Bursaphlenchus xylophilus and associated bacteria
2025 - IRG/WP 25-11057
Bursaphelenchus xylophilus or the pine wood nematode (PWN) is the causal agent of pine wilt disease, a destructive disease that affects coniferous masses. B. xylophilus causes severe economic, environmental, and social damage. The establishment of the disease depends on the ability of the PWN to degrade the lignocellulolytic material. Therefore, the study of enzymes able to hydrolyze these compone...
L Robertson, G Cebrián, D Gámez, L Fiorentini, S Rames, S Santos, M T Troya
Interaction of cellulolytic enzymes with fungal cell wall polysaccharides
2025 - IRG/WP 25-11071
Wood rotting fungi are the primary agents responsible for the decomposition of wood in natural environments. These fungi secrete a variety of enzymes to degrade the chemically and structurally resistant components of the wood cell wall. Some of these enzymes possess a carbohydrate-binding module (CBM), which enhances enzymatic efficiency by increasing their affinity to the substrate. Previously, w...
K Fukabori, N Hattori, Y Kojima, R Iizuka, M Yoshida
Physiological characterization of a key enzyme involved in gaseous COS assimilation in the filamentous fungus Trichoderma harzianum
2025 - IRG/WP 25-11072
Filamentous fungi are among the primary organisms responsible for the biological deterioration of wood, including wood decay and surface contamination. Therefore, understanding their physiological mechanisms is important for developing effective wood preservation strategies. Most physiological studies on filamentous fungi have focused on the metabolism of major elements, such as carbon in the form...
R Iizuka, A Ono, T Suzuki, Y Katayama, M Yoshida