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Improvements of monitoring the effects of soil organisms on wood in fungal cellar tests
1996 - IRG/WP 96-20093
Accelerated testing the durability of preservative treated timber in a so called "fungal cellar" or "soil-bed" to evaluate its performance in ground contact is widespread practice. In order to obtain a more accurate and reproducible estimate of preservative performance, several institutes, among them the BAM in Berlin, have routinely carried out static bending tests in addition to visual examination. These tests were usually performed with a defined maximum load or deflection path regardless of the remaining degree of elasticity of the test specimens. Recent studies at the BAM revealed that by modifying the method, i.e. by restricting the applied load to the non-destructive interval for each individual test specimen, the calculated modulus of elasticity (MOE) reflect the changing strength properties caused by biological deterioration and allow within a relatively short time valuable predictions on the service life of the treated timber in soil contact.
I Stephan, S Göller, D Rudolph

Japan's comments on ISO/DIS 12583-1/2
1996 - IRG/WP 96-20100
The paper describes an accelerated field test for the evaluation of timber preservative formulations against subterranean termites. The method has been adopted by the South African wood preservation industry as a screening method for the approval of wood preservatives for use under SA conditions. The method which is based upon the fungal cellar test offers a rapid means of evaluating the comparative performance of new wood preservative formulations in an environment that accurately reflects field conditions.
P Turner, D Conradie

Comparison of Different Methods for Assessing the Performance of Preservatives in the BAM Fungus Cellar Test
1998 - IRG/WP 98-20149
The fungus cellar test is a common means to get reliable data on the long term performance of treated wood in soil contact. A constantly high humidity and a suitable of water holding capacity for a range of micro-organisms provide high decay rates in untreated wood and produce intensive microbial pressures on wood treated with biocides. Presently a range of biocides are under test in the BAM fungus cellar and the results will be presented for the following types of biocides: Tebuconazole in combination with copper and boron (5 years fungus cellar), quats with copper and boron (5 years fungus cellar) and Cu-organic compound combined with copper and boron (3 years fungus cellar). Figures will be shown on the development of the Modulus of Elasticity (MOE) over the years and on an assessment of the stakes according to EN 252.
I Stephan, M Grinda, D Rudolph

Performance of Tebbacop in laboratory, fungus cellar and field tests
2000 - IRG/WP 00-30222
A novel organo-copper wood preservative ("Tebbacop") has been exposed to 12 years fungus cellar testing and 6 years above ground field testing. In the fungus cellar, Tebbacop at a retention of 0.053% Cu m/m oven dry wood out-performed CCA at a retention of 0.55% m/m oven dry wood. In above ground tests, L-joints treated to a Tebbacop retention of 0.012% Cu are performing as well as joints treated with TBTN to a retention of 0.08% Sn. In a slightly more severe decking test, 0.016% Cu as Tebbacop was more effective than 0.08% Sn as TBTN.
M E Hedley, D R Page, B E Patterson

The accelerated field simulator (= fungal cellar)
1982 - IRG/WP 2170
G C Johnson, J D Thornton, H Greaves

Protocol for evaluation and approving new wood preservative
1985 - IRG/WP 2159
M E Hedley, J A Butcher

Recent soft-rot research in softwoods and hardwoods
1980 - IRG/WP 1108
The purpose of this paper is to describe briefly the current status of our research on soft-rot fungi. The work to be discussed is still in progress and any results described must be regarded as provisional.
J A Butcher

Effectiveness and synergistic effects between copper and polymer betaine
1996 - IRG/WP 96-30097
Different formulations of "Copper Amine" and Polymer Betaine were studied. During laboratory tests a synergism between both active ingredients against soft rot and dry rot has been found. The efficacy against soft rot according to the "BAM method" and the European Standard ENV 807 depends only on the amount of copper. Long term tests in a fungus cellar for determining the relative protective effectiveness in ground contact show similar results as CCA-treated wood.
H Härtner, V Barth

Are fungal cellar tests really necessary?
1989 - IRG/WP 2333
During the past decade the range of methodology used to evaluate wood preservative potential has significantly expanded. At the forefront of these new tools available to the scientist·is the fungal cellar. This technique, as currently applied, involves the exposure of treated and untreated samples to conditions of moisture and temperature which ensure optimum fungal attack. By comparison data with that obtained on similar preservative systems in conventional stake tests, acceleration factors for predicting performance have been developed. This discussion paper examines the philosophy influencing the development of fungal cellar testing, and provides an alternative viewpoint.
J N R Ruddick

A combination of chlorothalonil and chlorpyrifos for more effective wood preservation
1995 - IRG/WP 95-30067
Chlorothalonil (tetrachloroisophthalonitrile) is a highly effective fungicide and wood preservative. Chlorpyrifos (O,O-diethyl O-(3,5,6-trichloro-2-pyridinyl)phosphorothioate) is an insecticide and is not generally considered to possess significant antifungal activity. However, a review of the literature revealed that chlorpyrifos effects in various ways the activity of microorganisms and is even registered for control of a fungal plant disease. Laboratory and field research trials were designed to study the wood preservative effectiveness of combinations of chlorothalonil plus chlorpyrifos. In some tests the antifungal activity of the insecticide chlorpyrifos was confirmed; however in a fungus cellar test, chlorpyrifos by itself provided little protection. In laboratory termite testing, chlorothalonil was sufficiently effective that in most experiments the addition of chlorpyrifos had little effect, while in field tests the combination consistently outperforms chlorothalonil alone. In tests against various marine borer species, the combination of chlorothalonil plus chlorpyrifos proved superior to chlorothalonil alone. The synergy of chlorothalonil plus chlorpyrifos is demonstrated in field stake tests, where after eleven years in Florida the combination has given superior performance over stakes treated with pentachlorophenol or chlorothalonil alone. Chlorpyrifos alone provided little protection against decay in these field stake tests. Chlorpyrifos is a useful additive to wood preservative fungicides, conferring additional protection against both decay fungi and wood destroying insects.
T L Woods, P E Laks, T C Blewett, R D Fears

Fungus cellar and stake tests with tall oil derivatives. Progress Report after 5 years' testing
1987 - IRG/WP 3442
Two derivatives of tall oils have been tested for five years in fungus cellar and stake tests. The samples were relatively quickly attacked on the surface by decay fungi, mainly soft rot, but the decay has progressed very slowly. The performance of the stakes in the tests has so far been equivalent or even better than some CCA preservatives and creosote.
J Jermer, Ö Bergman, T Nilsson

The interaction of polyflavonoid tannins with CCA in Pinus radiata
1987 - IRG/WP 3422
Polyflavonoid tannins complex easily and rapidly with metal ions such as copper, chromium, and arsenic. such complexes in high-tannin-containing CCA treated hardwoods might result in essentially under-treated timber. Four aspects of this interaction were investigated: 1) The relationship between tannin contents of seven hardwoods (Betula pendula, Alstonia scholaris, Fagus sylvatica, Liquidambar styraciflua, Nothofagus menziesii, Platanus acerifolia and Tilia vulgaris) and one softwood (Pinus radiata) and% Cu toxic thresholds; 2) Determination of tannin distribution within cell wall layers; 3) The field performance of Pinus radiata treated with solutions of bark tannin, then CCA; 4) TEM/EDAX studies on CCA loadings in the S2 layer of tannin/CCA treated Pinus radiata tracheids. Our observations suggest that by themselves tannins have a minor influence on field performance of CCA-treated hardwoods.
K G Ryan, D V Plackett

Comparison of decay rates of preservative-treated stakes in field and fungus cellar tests
1980 - IRG/WP 2135
With the exception of acid-copper-chromate, zinc-chrome-arsenate, and sodium pentachlorophenoxide, the relative performance of preservatives in the fungus cellar was similar to that in the field.
M E Hedley

Questionnaire: Facility for accelerated stake tests in unsterile soil
1981 - IRG/WP 2166
An acceptable name for this type of test facility has not yet been devised, but it has previously been referred to as a FUNGUS CELLAR or as a TROPICAL DECAY HOUSE. The attached pamphlet (What's New in Forest Research No.65) describes in broad terms the facility used for this purpose at FRI in New Zealand. Over the last two or three years several laboratories and commercial firms have installed so-called fungus cellars. At the 1981 I.R.G. Meeting in Sarajevo it was proposed that a survey be undertaken to obtain basic information on the various developments in this new area of biological testing. To this end, I shall be pleased if you complete the enclosed questionnaire and return it to: Dr J.A. Butcher, Forest Research Institute, Private Bag, Rotorua, NEW ZEALAND. A full report on this survey will be presented at the next I.R.G. meeting scheduled for Turkey in May 1982.
J A Butcher

Soil-bed studies
1982 - IRG/WP 2181
This paper discusses factors affecting the design and use of a soil-bed.
P Vinden, J G Savory, D J Dickinson, J F Levy

Natural durability studies in an accelerated field simulator - A novel approach
1983 - IRG/WP 2197
A study of the natural durability of untreated timbers to both decay and termite attack is described. The work illustrates the versatility of the Accelerated Field Simulator as a novel approach to biodeterioration research.
G C Johnson, J D Thornton, J W Creffield, C D Howick

Report on questionnaire: Facility for accelerated stake tests in unsterile soil
1983 - IRG/WP 2169
In October 1981 a questionnaire (IRG Doc. No. IRG/WP/2166) on the so-called fungus cellar tests in unsterile soil was prepared and despatched to 56 individuals representing various institutes, organisations, or companies. In addition a further 68 letters describing the purpose of the questionnaire were sent to additional IRG members who could request the full questionnaire if appropriate. This represented a contact with 125 individuals in 48 countries. A verbal report on the results of this survey was presented at IRG 13 in Cesme, Turkey. The present document formalises that report.
J A Butcher

Comparison of the effect of different soil sources on the type and rate of decay of CCA-treated pine exposed in a soil-bed
1984 - IRG/WP 2213
The types of decay observed in CCA-treated pine posts in horticultural situations in New Zealand can be reproduced using a soil-bed exposure. Radiata pine stakelets, untreated or treated to 1.4, 2.7, or 5.4 kg/m³ with Tanalith NCA, were exposed to six different soil sources. The local nursery soil used for all standard laboratory tests was found to represent the greatest decay hazard to untreated pine. Poverty Bay horticultural soils were more hazardous than the local field test site soil to CCA-treated pine. Soil collected from adjacent to a 'decaying' post could decay treated wood faster than soil collected from around a 'sound' post. Brownrot, softrot, and bacterial degrade was observed. All failures of untreated pine and early failures of CCA-treated pine were caused by brownrot.
J A Drysdale

Comparison of decay rates of preservative-treated stakes in field and fungus cellar tests. Results after 40 months fungal cellar exposure
1983 - IRG/WP 2200
Decay rates of preservative-treated Pinus radiata stakes during 40 months exposure in the FRI fungus cellar were compared with those of similarly treated material in a field test. Decay rates in the fungus cellar were from 4 to 100 times higher than in the field, although for the majority of preservatives the rate was between 7 and 12 times higher. The lag phase before onset of decay, noticeable with most of the preservatives in the field test, was largely eliminated in the fungus cellar. Possible reasons are given for inconsistencies in relative rates of decay of preservatives in the two tests.
M E Hedley

Soil-bed studies. Part 2: The efficacy of wood preservative
1983 - IRG/WP 2205
Various methods of decay assessment were investigated. Three stages or phases of decay were identified which could be used to describe the efficacy of a preservative system or virulence of a soil-bed testing medium. These included the lag, decay, and senescent phase. Premature senescence could arise if wood samples became waterlogged. It was concluded that time to failure was unsuitable as a method of decay assessment. Decay assessment by deflection testing was therefore limited to assessing linear losses in elasticity against time.
P Vinden, J F Levy, D J Dickinson

Fungus cellar and field tests with tall oil derivatives. Final report after 11 years' testing
1993 - IRG/WP 93-30007
Two derivatives of tall oil were tested as wood preservatives in a fungus cellar and in ground contact (field test). Stakes of Pinus sylvestris sapwood were used in the tests. For the field test the size of the stakes was 20 x 50 x 500 mm³ and for the fungus cellar test 20 x 20 x 250 mm³. The stakes were vacuum-pressure treated with the two products and exposed in 1981. The field test site used was in Uppsala, where the soil type is clay. In 1991 the last stakes in the field test were rejected and in 1992, the last stakes in the fungus cellar failed. The effect against biological degradation of the two products is compared with that of wood preservatives in current use.
J Jermer, Ö Bergman, T Nilsson

Fungus cellar testing as an evaluation method for performance of treated timber in ground contact
2001 - IRG/WP 01-20227
A fungus cellar method for the accelerated evaluation of performance of treated wood in ground contact is described. The test soil comprised of sandy loam, vermiculite and Japanese horticulture soil "Kanumatsuchi" in a ratio of 6:2:2 by volume. The soil was inoculated with the dominant test fungus isolated with selective medium from decayed wood samples. Pairs of treated and untreated wood specimens Japanese cedar in contact with each other were buried vertically for two thirds their length. Assessment of the specimens was carried out periodically using the FFPRI graveyard damage index six- grade scale. Important factors for accelerating decay were the moisture control of the mixed soil, temperature and relative humidity, and the maintenance of fungus activity. The fungus used for inoculation favored a soil water holding capacity (WHC) of 50-80%. Under these conditions the untreated control specimens had a damage index in six months equivalent to three years FFPRI graveyard test service life, the decay accelerating rate by the fungus cellar to the graveyard test was 6 times. Under higher soil moisture conditions (WHC>80%) in the fungus cellar, soft rot was dominant and the decay rate was slower. DDAC treated specimens (8.4kg/m3) had a damage index of 2.6 in three years and 3.0 in four years. DDAC treated specimens (8.2kg/m3) in the graveyard test have been shown to be durable for 12 years (damage index of 2.3 in ten years and 2.6 after 12 years). This fungus cellar method has been shown to accelerate decay in DDAC treated specimens 3 times or more in comparison to the FFPRI graveyard test. On the other hand specimens treated with Copper-azole (6.0kg/m3 as retention of actives) had a damage index of 0 after eight years. The average service life in the FFPRI graveyard test is not decided as the Copper-azole treated specimens are in sound condition so it is not yet possible to evaluate the accelerating rate for the Copper-azole by the fungus cellar method. The fungus cellar method will be an useful method for the accelerated evaluation of performance of treated wood in ground contact provided the test conditions can be controlled.
Y Nagano

Assessment of the toxicity of some copper-, zinc- and boron-based wood preservatives to the cellar fungus Coniophora cerebella Schröet
1974 - IRG/WP 242
This article reports the use of a method based on the determination of the probability of the protection of timber against destruction by fungi. By converting the probability values to probit values and plotting them as a function of the amount of preservative retained in the timber, curves of the toxic effect are obtained, enabling any timber protection probability to be assessed.
V N Sozonova, D A Belenkov

The use of modulus of rupture and modulus of elasticity in natural durability testing
1997 - IRG/WP 97-20117
Losses in weight, losses in bending strength and changes in elastic behaviour were assessed in a fungus cellar test with beech wood stakes (Fagus sylvatica). Results were gained after 8, 12, 16 and 20 weeks resp . The outcomes show, that the non-durable species beech is very rapidly attacked by fungi and loses up to 60% of its initial bending strength even within the first 8 weeks. Earlier research by Militz et al. (1996) showed that a durable species in the same period and under comparable conditions do not have any strength reduction. An assessment of weight loss lead to mass losses of ca. 20 - 30% within the same period. From the results it can be stated, that the use of MOR instead of or in combination with weight loss assessments have some advantages to the solely use of weight loss assessments. Furthermore, the outcomes show that as a non-destructive method, MOE assessments are a good tool in the prediction of fungal attack. For the inspection of field trials, the dynamic vibration method can offer some advantages, because no laboratory equipment is needed and the measurements only take some seconds. Further research is ongoing to gain more knowledge on the role of varying moisture contents of stakes in field trials on this type of MOE assessment.
L Machek, H Militz, W Gard

Observations on the performance of copper-based wood preservatives in fungal cellar (soil-bed) tests
1994 - IRG/WP 94-20047
Fungal cellar (soil-bed) tests are considered to be an important tool for the evaluation of the performance of ground contact wood preservatives. Facilities of this type have been established world wide although caution has been exercised in their introduction into standard testing methods for the approval of wood preservatives. This is the result of concerns over the variability in the biological activity between different facilities, and thereafter the determination of effective preservative retentions. This paper presents results of tests on copper based wood preservatives from four different fungal cellar facilities. The results show consistent trends in preservative performance and the high decay rates demonstrate the value of this type of test in determining the potential of new wood preservatives for long term protection in ground contact.
G R Williams, D Rudolph, M E Hedley, J A Drysdale, R F Fox

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