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In vitro sporulation of selected wood decay fungi
1978 - IRG/WP 190
Basidiospores produced axenically without laborious attention in the laboratory are useful in studies of wood decay initiation. Such spores presumably approach those collected from natural sporophores in size and germinability (Morton, 1964). Production of spores in vitro by inversion of cultures grown on 2% malt extract agar in deep glass dishes (100x80 mm²) has been the preferred method (Morton...
E L Schmidt, D W French


Moisture requirements and wood degradation of pine and spruce wood by some Basidiomycetes-fungi
1989 - IRG/WP 1406
The minimum moisture requirements for the growth of the brown rot fungi, Coniophora puteana, Gloeophyllum trabeum and Serpula lacrymans were near or above the fibre saturation point of pine and spruce, sap and heartwood, during 160 days incubation according to the standard method EN 113. The moisture of wood for the optimum growth of Coniophora puteana and Serpula lacrymans was about 35-80% and fo...
H Viitanen, A-C Ritschkoff


Improvement of wood decay resistance by tannin impregnation
1991 - IRG/WP 2380
The fungical effect of tannins is appraised after exposure of treated woods samples to wood rotting fungi. A screening-test is carried out with several solutions containing extracts of two kinds of tannins; a condensed tannin derived from Quebracho heartwood (Schinopsis sp.) and a hydrolyzable one extracted from chestnut heartwood (Castanea sativa Mill). If a fungicidal action was indeed found it ...
D Dirol, A Scalbert


Contradiction between uptake of preservative in practical situation and laboratory testing
1992 - IRG/WP 92-2392
By brush treatment water- and solvent-based products were applied on boards of Scots pine and Norway spruce to achieve an uptake of 100 and 200 g/ml. In addition the scope of work to achieve the required uptake of preservative was determined under practical conditions for both product groups. EN 113 (RAL-GZ 830) tests were carried out to test the biological efficacy of products. After 18 months ex...
R Gründlinger, O Janotta, M Melzer


A serial exposure technique for estimating probable service life of treated timber
1978 - IRG/WP 2111
This paper briefly describes part of our preliminary work aimed at developing a test procedure that culd be adopted as a standard method. A detailed version of the work has been submitted for formal publication. In both papers, the aim is to promote interest in extended laboratory testing of wood preservatives. It is believed that this work may provide the basis of a laboratory test procedure from...
J A Butcher


Low polymer levels containing bioactive monomer polymerized in situ provide resistance to Gloeophyllum trabeum
1995 - IRG/WP 95-30066
Wood preservation based on in situ polymerization of potentially bioactive monomers has been studied. Tributyltin oxide acrylate (TBTOA) and pentachlorophenol acrylate (PCPA) were synthesized. Wood samples were treated at 2, 5, 10, 15 and 20% by weight solutions with varying amounts of crosslinker (trimethylolpropane trimethacrylate, TMPTM) and polymerized in situ in wood samples (2.54 x 2.54 x 0....
R E Ibach, R M Rowell


Effect of cyclic change of temperature on fungal growth and mass loss
1994 - IRG/WP 94-10065
For estimating the effect of cycling change of temparature on fungal growth, four Japanese species, sugi (Cryptomeria japonica), hinoki (Chamaecyparis obtusa), akamatsu (Pinus densiflora) and buna (Fagus crenata), four fungal species, Tyromyces palustris, Gloeophyllum trabeum, Coriolus versicolor, Pycnoporus coccineus, and five temperature conditions, 10, 20, 27, 35°C and cyclic temperature (20°...
K Suzuki, K Okada


Soft-rot control in hardwoods treated with chromated copper arsenate preservatives. Part 3: Influence of wood substrate and copper loadings
1977 - IRG/WP 2100
The hypothesis is proposed that hardwoods need more chromated copper arsenate (CCA) than softwoods to protect them from soft-rot attack mainly because hardwoods are more readily consumed by soft-rot fungi. Simple model systems, using copper-supplemented agar or groundwood pulp treated with CCA showed that fungi tolerated more toxicant (copper) as more available substrate (malt) was provided. Soft-...
M A Hulme, J A Butcher


Evaluation of bacteria for biological control of wood decay
1990 - IRG/WP 1426
Laboratory soil-block and agar-block tests were carried out to evaluate the efficacy of bacteria as biological control agents against 5 brown-rot and 3 white-rot fungi. Pretreatment of Southern pine and sweetgum with a bacterial solution prevented decay in agar-block tests. However, the bacteria were generally ineffective in preventing decay in Southern pine, Douglas-fir, sweetgum and yellow popla...
R Benko, T L Highley


Evaluation of chlorpyrifos and fungicides alone and in combination for control of insects and fungi in wood and wood composites
1998 - IRG/WP 98-30187
Wood composites are rapidly being adapted for use in exterior applications. The incorporation of a preservative system to prevent fungus and insect attack is necessary with most exterior composites. Research studies were reviewed pertaining to organic preservative systems based on Lentrek* insecticide wood treatment which contains the active ingredient chlorpyrifos alone and in combination with te...
M P Tolley, P E Laks, R Fears


Efficacy of anhydrides as wood protection chemicals
1997 - IRG/WP 97-30162
Wood samples treated with anhydrides of various compositions have been examined for their decay resistance and moisture behaviour. For this purpose two brown rot fungi (Coniophora puteana and Gloeophyllum trabeum) and two white rot fungi (Trametes versicolor and Pycnoporus sanguineus) were used in conventional and modified soil block tests. The modified soil block tests conformed to the method as ...
S C Forster, M D C Hale, G R Williams


Non-destructive stress wave measurement of decay and termite attack in experimental wood units
1986 - IRG/WP 2256
The purpose of this study was to determine if stress wave analysis could be used to monitor the degradation of wood specimens exposed to the brown-rot decay fungi (Gloeophyllum trabeum) and of wood specimens subjected to attack by subterranean termites. One hundred fifty 3/4 by 3/4 by 12 in. Southern pine specimens were used for exposure to brown-rot decay fungi and two hundred twenty-five 3/4 by ...
R F Pellerin, R C De Groot, G R Esenther


Evaluating the potential of amine chemicals for use as wood protecting agents. Part 1: Investigation of cation components of quaternary ammonium compounds
1994 - IRG/WP 94-30049
Quaternary ammonium compounds (quats) have shown a great potential as more environmentally acceptable wood preservatives. In order to identify chemicals possessing the wood protecting potential, an evaluation was carried out of a range of commercially available 'quats', using a modified soil block test. Ponderosa pine sapwood blocks were treated with selected 'quat&a...
Hang Tang, J N R Ruddick


Targeted inhibition of wood decay (Using everything but the kitchen sink)
1997 - IRG/WP 97-10203
Low molecular weight oxidative decay agents have been implicated in the degradation of wood by brown-rot decay as evidenced by chemical analysis of brown-rotted wood and detection of oxalic acid and hydroxy radicals. Fenton chemistry (H2O2 / Fe++) is often proposed as the mechanism for generating hydroxy radicals. Previous authors have shown iron to enhance the brown-rot hydrolysis of wood, while ...
F Green III, T A Kuster, T L Highley


The biological effectiveness of wood modified with heptadecenylsuccinic anhydride against two brown rot fungi: Coniophora puteana and Gloeophyllum trabeum
1992 - IRG/WP 92-3705
A modified soil block test was carried out using wood samples reacted with heptadecenylsuccinic anhydride (ASA). This modification gave good resistance to decay brought about by the brown rot fungi Coniophora puteana and Gloeophyllum trabeum during the twelve week exposure period. Results indicated that there was a good correlation between increased loading of modifying reagent and an increase in ...
C Codd, W B Banks, J A Cornfield, G R Williams


Utilization of coconut timber from north Sulawesi, Indonesia. Part 1: Durability
1994 - IRG/WP 94-30044
Coconut timber from non-productive plantations is a byproduct of an agricultural crop and, by the same token, constitutes a renewable resource which may serve as a complement to or, at least in part, as a substitute for traditional timbers in local markets. Its more extensive utilization is expected to contribute to the conservation of tropical rain forests. Export of coconut timber and/or wood pr...
R-D Peek


Resistance to decay of particle boards: Presentation of a test method
1986 - IRG/WP 2260
The experimentations were undertaken on particle boards 35 and 50 mm thick. The rot resistance tests were carried out on specimens whose dimensions were 600 x 75 x 35 mm³ for particle boards 35 mm thick and 800 x 75 x 50 mm³ for particle boards 50 mm thick according to a method derived from the one described in the documents IRG/WP/2214 and IRG/WP/2243. Two exposure periods were used: 12 and 16 ...
G R Y Déon, N Trong


Chelator production and cellobiose dehydrogenase activity of wood inhabiting fungi
2000 - IRG/WP 00-10363
Multiple components have been postulated to be involved in the Fenton-based biological degradation of wood by brown rot fungi. Chelators have been implicated in essential iron reduction steps. Cellobiose dehydrogenase activity and quinone reducing activity have also been implicated in brown rot biodegradation. When biochelator production by three white rot fungi, three brown rot fungi and three wo...
Weihong Qi, J Jellison


A test method to simulate above-ground exposure
1978 - IRG/WP 2112
The simulated above-ground exposure technique described is worthy of consideration as a simple procedure to determine the relative decay resistance of preservative-treated wood exposed to a moderate decay hazard. The method may readily be modified to allow testing of other materials such as plywood by simply enlarging the slots cut in the modified feeder strips. The method by no means completely s...
J A Butcher


Differences in pH, electrical resistance, cation composition and NIR spectra of red spruce wood during early stages of brown rot degradation
2002 - IRG/WP 02-10449
Red spruce sapwood was exposed to degradation by the brown rot fungi Coniophora puteana, Postia placenta, Gloeophyllum trabeum and Serpula lacrymans for 0, 1, 2 or 3 weeks using a modified soil block assay design. Average weight losses over time ranged from 0-8.9% during this time period. Detectable changes in pH, electrical resistance and cation compostion were observed in the wood as early as 1 ...
J Jellison, S Kelley, B Goodell, D Hui, A Ostrofsky


Regulation of hyphal sheath formation iron-chelator production by the brown rot fungi Gloeophyllum trabeum and Postia placenta
1994 - IRG/WP 94-10074
Hyphal sheath formation and iron-chelator production by liquid cultures of the brown rot fungi Gloeophyllum trabeum and Postia placenta were monitored over time. The effects of incubation at various temperatures, pH's and concentrations of iron, manganese and nitrogen were determined. Hyphal sheath production was observed microscopically and quantified with JAVA video analysis. The hyphal...
Y Chen, J Jellison


Efficacy of fumigants in the eradication of decay fungi implanted in southern pine timbers
1986 - IRG/WP 3365
Southern pine timbers (15.2 cm x 15.2 cm x 4.26 m) were fumigated at midlength to evaluate the effectiveness of the fumigants chloropicrin, Vapam, Diazomet, Busan 40, Vanicide 51, Vanicide TH, sodium bisulfite, and ethanolamine in eradication of decay fungi. The fumigants were introduced into 2.54 cm holes which were closed immediately with rubber stoppers. Movement and persistence of lethal conce...
T L Highley, W E Eslyn


Extracellular hydrogen peroxide-producing and one-electron oxidation system of brown-rot fungi
1990 - IRG/WP 1445
Wood-component-degrading compounds involved in the initial degradation of the cellulose and lignin in wood were isolated from wood-containing culture of brown-rot fungi, Gloeophyllum trabeum and Tyromyces palustris and partially purified by gel filtration on Sephadex G-25 and DEAE-Sepharose ion-exchange chromatography. The compounds were glycoproteins. The molecular weights of the glycoproteins as...
A Enoki, S Yoshioka, H Tanaka, G Fuse


Solid state NMR study on pine wood degraded by brown rot fungus Gloeophyllum trabeum
1994 - IRG/WP 94-10067
Blocks of Pinus koraiensis wood were degraded by Gloeophyllum trabeum and examined by solid-state 13C NMR spectroscopy. Chemical compositions were estimated from relative signal aras. There was no evidence for preferential degradation of the noncrystalline cellulose which accounted for about 4% by weight of the wood. Loss of partly ordered cellulose on crystal surfaces exceeded the loss of crystal...
Yoon Soo Kim, R Newman


Use of compression strength loss for measuring decay in the soil block test
1996 - IRG/WP 96-20083
The possibility of using radial direction compression strength of wood, rather than mass loss, was evaluated for both a brown-rot fungus (Gloeophyllum trabeum) and white-rot fungus (Trametes versicolor). With untreated pine wafers (5 x 19 x 19 mm³ - l x r x t) exposed to Gloeophyllum trabeum in a soil block test for five days, the compression strength loss was nearly 50% compared to a 5% mass los...
D D Nicholas, Zhongwei Jin


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