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Growth of selected wood decay fungi on various agar-supplemented media
2003 - IRG/WP 03-10456
The growth rates of a selection of wood decay fungi (brown and white rots) on various agar-supplemented media have been determined and compared. The agar media investigated were Malt extract agar (MEA), Potato dextrose agar (PDA), YMPG agar (yeast extract, malt extract, bacto-peptone, glucose, asparagine and thiamine), YMPG agar (without amino acids) and Beech wood powder agar (BWA). The tested wo...
S A Amartey, M Humar, F Pohleven


The effect of Trichoderma volatiles on the growth and enzyme production of Serpula lacrymans
2000 - IRG/WP 00-10332
Although various authors have reported the biological control of Serpula lacrymans by Trichoderma spp. the mode of antagonism employed by Trichoderma is not yet clear. The work presented here concentrates on the production of anti-fungal volatiles for inhibition of S. lacrymans growth. Volatile mediated interactions were examined between four S. lacrymans isolates and a range of nine known Trichod...
S N Humphris, A Bruce, R E Wheatley


Respiration measurement of dry-rot
1995 - IRG/WP 95-10095
Methods for measuring the production of carbondioxide from wood infected with the dry-rot fungus were evaluated. By enclosing whole wood blocks and measuring the concentration of gas in the headspace by gas chromatography, an exponential increase in the concentration of CO2 was observed for at least 2 months. This technique could therefore present a method for evaluating various treatments of dry-...
L Toft


A non-pressure method of protection based on hurdle theory to control the spectrum of internal environmental factors which affect the decay of poles in soil contact
1993 - IRG/WP 93-20005
A field trial was conducted to establish whether superficial barrier linings on poles in soil contact could function as environmental hurdles against the growth of biological agents and thus provide preventative methodology to preclude premature failure of vineyard poles under flood-irrigation. Assessment after 52 weeks exposure to the prevailing conditions and sub-tropical environment showed that...
A A W Baecker


Effects of cyproconazole and copper sulphate on the length of the hyphal growth unit (HGU) of the white-rot fungus Coriolus versicolor
2003 - IRG/WP 03-10473
Wood decay basidiomycetes have been shown to produce appreciable quantities of extracellular mucilaginous materials (ECMM). The relationship between ECMM and total biomass production has been investigated in the white-rot fungus Coriolus versicolor (CTB 863 A). Differences in the amount of ECMM produced by the fungus proportionally to the total biomass, were observed under a range of physiological...
D Vesentini, D J Dickinson, R J Murphy


Blue stain in service on wood surface coatings. Part 1: The nutritional requirements of Aureobasidium pullulans
1992 - IRG/WP 92-1556
The nutritional requirements of Aureobasidium pullulans was examined with regard to nutrient sources that are potentially available in fresh and weathered wood. The study was designed to determine how far wood cell wall components need to be broken down during weathering before they provide a useful nutrient source for Aureobasidium pullulans. Various carbon sources were tested, with eight differe...
P R Sharpe, D J Dickinson


Soil treatment tests with the three products of boric acid for the prevention of the hyphal growth of Serpula lacrymans
1992 - IRG/WP 92-3693
Laboratory soil treatment tests were conducted for the evaluation of fungicidal or fungistatic effect of boric acid products against Serpula lacrymans. Boric acid products tested were the following three: a thickened boric acid solution in triethanolamine, boric acid-silica gel complex granules, and a nonwoven fabric laminated with a polypropylene film and coated with boric acid granules on one si...
S Doi, A Yamada, Y Mineki, M Mori


Growth of sapstain fungi in scots pine and the effect of timber ageing
1998 - IRG/WP 98-10269
A trial was initiated to study the growth of known isolates of sapstain fungi in scots pine (Pinus sylvestris) which had been naturally aged or sterilised by autoclaving or irradiation. Sawlogs cut from commercial thinnings were reduced to billet size and the exposed ends were painted with a bituminous sealant to reduce moisture loss. Some billets were inoculated after autoclaving or gamma-irradia...
N J Strong, J F Webber, R A Eaton


An attempt to grow dry rot fungus Serpula lacrymans on mortar substrate in non-sterile conditions
1989 - IRG/WP 1385
According to many experts, Serpula lacrymans is probably the most dangerous wood destroying fungus in houses. Investigations of KOCH, A.P. (2) showed that incidence of this fungus in percent was around 20% when the frequency of some other standard fungi was far below (Coriolus versicolor: 0.2% for example). The work presented here is a part of a program carried out at CTFT for assessing the resist...
L N Trong


A rearing procedure for the house longhorn beetle Hylotrupes bajulus L
1973 - IRG/WP 228
The technique practised at the Princes Risborough Laboratory for culturing Hylotrupes bajulus is described. It is modified from methods previously suggested by German workers. A protein and vitamin enriched wood diet to accelerate larval growth is employed and larvae are subsequently given a period of cool storage to induce pupation. Adults are confined in groups with prepared surfaces for egg lay...
R W Berry


Effect of humidity fluctuations on mould growth on pine wood
1989 - IRG/WP 1412
Most studies on mould development on wood material have been made at constant moisture conditions during the experimental period. As a consequence of such studies, a maximum permittable humidity level to avoid mould problems in wood constructions has been recommended. In the practical situation, the constructions are seldom subject to constant humidity or temperature. In the present paper, we repo...
J Bjurman


Organic nitrogen in wood: Growth substrates for a sapstain fungus
1993 - IRG/WP 93-10019
The nitrogen content of mature wood from lodgepole pine was 0.046% as determined by micro-Kjeldahl. Low molecular weight proteins between 14 and 21 kDa were identified in mature wood, branch wood and sap pressed from wood chips. Amino acids were quantified in acid-hydrolyzed wood samples and accounted for between 19 and 35% of the nitrogen in wood. The sapstaining ascomycete Ophiostoma piceae stra...
L D Abraham, C Breuil


Growth of the copper tolerant brown rot fungus Antrodia vaillantii on different substrates
1995 - IRG/WP 95-10121
In recent years the copper tolerant brown rot fungus Antrodia vaillantii caused severe damages on impregnated wood in ground contact. The pattern of decay gave the impression that impregnated wood was even more severely attacked than unimpregnated. To investigate this question more closely laboratory tests were carried out. In a "choice test" Antrodia vaillantii grew preferably towards CC-impregna...
H Leithoff, I Stephan, M-T Lenz, R-D Peek


Effects of Prior Establishment of Trichoderma harzianum on Ophiostoma picea Growth in Freshly Sawn Douglas-fir Sapwood
2003 - IRG/WP 03-10476
Trichoderma harzianum has been shown to be an effective biocontrol agent against a number of wood inhabiting fungi under laboratory conditions, but this fungus has performed poorly in field trials. Understanding the interactions between biocontrol agents and their intended targets in wood may provide important clues for developing improved approaches to biocontrol, potentially reducing our relianc...
Ying Xiao, J J Morrell, L M Ciuffetti


Mould growth at lumber surfaces of pine after kiln and air drying
1994 - IRG/WP 94-40033
Distribution of water soluble substances in green wood and later redistribution during drying is of importance for the colonisation of wood by microorganisms. According to literature the availability of nitrogenous materials is probably a major limiting factor to the microbial colonisation of wood. King et al. (1974) and Oxley et al. (1976) have shown correlation between surface nutrient concentra...
N Terziev, J Bjurman, J B Boutelje


Hyphal tunnelling of belian (Eusideroxylon zwageri) wood cell walls
1996 - IRG/WP 96-10152
Belian (Eusideroxylon zwageri) is highly regarded among the naturally durable Malaysian hardwoods. Visual inspections at the groundline of belian transmission poles from Sarawak, Malaysia, showed only surface decay of wood after 20 years in service. The cause of decay in belian was investigated by light and transmission electron microscopy. Light microscopy of transverse sections of surface tissue...
A P Singh, A H H Wong


Variations in the virulence of test strains of Coniophora puteana (Schum ex Fr) Karst
1982 - IRG/WP 2185
In laboratory experiments using petri-dishes and small wood blocks (30 x 10 x 5 mm³) 6 different isolates of Coniophora puteana strain BAM 15 were compared with strain FPRL 11E for growth rate and decay capability. Only the recently received strain from EMPA was suitably active giving 24% weight loss after 6 weeks compared with 38.1% for 11E. Collation of data from standard test records revealed ...
A F Bravery, J K Carey, W Worley


Characterization of Poria indoor brown-rot fungi
1995 - IRG/WP 95-10094
The heterogeneous group of "Poria" fungi causing brown rot in buildings and also of wood in ground contact comprises Antrodia vaillantii, Antrodia serialis, Antrodia sinuosa, Antrodia xantha and Tyromyces placenta. These fungi have similar morphological appearance and biology. Their nomenclature has a confusing history and is still not uniform. As a consequence, misinterpretations may occur. SDS p...
O Schmidt


Effect of crude tall oil, linseed oil and rapeseed oil on the growth of the decay fungi
2002 - IRG/WP 02-30299
The influence of crude tall oil, linseed oil and rapeseed oil on the growth of Coniophora puteana, Poria placenta and Coriolus versicolor was studied. The selected test oils were observed to have different effects on the the fungal growth. Crude tall oil inhibited the radial growth of all fungi. Rapeseed oil either accelerated or inhibited the growth of fungi depending on the type of fungus involv...
L Paajanen, A-C Ritschkoff


Gaseous treatment of timber with allyl isothiocyanate. Fungicidal and insecticidal effects
1996 - IRG/WP 96-30108
Gaseous treatment with allyl isothiocyanate (AIT) was tested for its effects on the growth of microorganisms on the wood substrate and the mortality of subterranean termites and powder-post beetles. Toxic limits of AIT were determined as concentrations in the air when an AIT-treated filter paper was placed in a sealed container with fungus-inoculated wood specimens. Those were <3.8 ppm for Aure...
K Tsunoda, T Yoshimura


Colonisation of painted wood by Aureobasidium pullulans - Analysis of features and consequences for failure in service
1996 - IRG/WP 96-10144
Wooden blocks of spruce were painted with different paint formulations. Water- and solvent-borne model and commercial paints were used. The painted wooden blocks were inoculated with a spore suspension of Aureobasidium pullulans (de Bary) Berkg. and placed in an environment of high humidity for 14 months. Different colonisation and growth patterns were observed on the different paint surfaces. The...
S L Bardage


Eradication of wood decay fungi by means of radio frequency
1998 - IRG/WP 98-10292
High frequency electromagnetic fields i.e. radio frequency (RF) are used in wood industry for heating, gluing and bending of wood and are also appropriate for eradicating of wood decay fungi and insects. We investigated the effects of RF exposure on wood samples which were in vitro infected by Coniophora puteana, Gloeophyllum trabeum and Lentinus lepideus. For each fungus, the lethal temperature a...
F Pohleven, J Resnik, A Kobe


The loss of insecticidal action from synthetic pyrethroid-treated wood samples: The effect of high temperatures and relative humidities
1992 - IRG/WP 92-1569
This paper describes the results from bioassays using Hylotrupes bajulus, and chemical analyses, of pyrethroid-treated wood samples following storage for up to 3 years. A range of four storage environments was used consisting of combinations of two temperatures (20°C and 40°C) and two relative humidities (60% and 90%). It is concluded from the chemical analyses that, although losses at room temp...
R W Berry, S J Read


The effect of added nutrients on growth rate and decay capacity of Serpula lacrymans
1990 - IRG/WP 1427
At the previous meeting a new technique was presented that enables both fungal growth rate and wood decay rate to be measured using the same timber specimen. The technique (IRG/WP/1384) has previously been carried out with 1% malt as the sole nutrient within the small jar that provides the inoculum for this method. Results presented here relate to an additional level of 5% malt, with or without a ...
J D Thornton, A McConalogue


Resistance of acrylic paints on wood against growth of the rot fungus Dacrymyces stillatus
1990 - IRG/WP 2345
In the last few years the presence of the rot fungus Dacrymyces stillatus has been repeatedly confirmed in external wood panels, particularly from wood painted with water based paints. A laboratory method for testing of the fungal resistance of paint films on wood has been developed.This method has been used to test the efficacy of the fungicides Parmetol DF 18 and Parmetol HF 25 against attack by...
J Bjurman


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