Your search resulted in 7 documents.
Immunogold labelling of size marker proteins in brown rot-degraded pine wood
1990 - IRG/WP 1428
Pine wood degraded by Fomitopsis pinicola was infiltrated with a mixture of ovalbumin (45 kDa) and myoglobin (16.7 kDa). After crosslinking of the proteins with glutaraldehyde and preparation for electron microscopy ultrathin sections were labeled with gold-conjugated antibodies. Simultaneous labeling of both proteins on the same section showed that at 50-70% weight loss ovalbumin did not penetrat...
E Srebotnik, K Messner
Use of fluorescent-coupled lectins as probes for studying fungal degradation of wood
1986 - IRG/WP 1288
The ability of the fluorescent-coupled lectins wheat germ agglutin (WGA) and Concanavalin A (Con A) to react with selected Basidiomycetes, Ascomycetes, and Fungi Imperfecti was evaluated using pure cultures of 35 fungi grown on malt extract agar. WGA, which binds specifically to the n-acetylglucosamine residues found in fungal chitin, reacted with nearly all hyaline fungal structures but did not r...
J J Morrell, R L Krahmer, L C Lin
Molecular analysis of the basidiomycete Coniophora puteana
1992 - IRG/WP 92-1534
Sodium dodecylsulphate polyacrylamide gel electrophoresis and Western Blotting, using a polyclonal antiserum produced against a whole cell extract of Coniophora puteana, were used to analyse the major proteins and antigens of the wet rot organism Coniophora puteana. The macromolecule profiles of this organism were different from other members of the Coniophora genus and from a set of unrelated org...
H E McDowell, D Button, J W Palfreyman
Reduction of preservatives leaching from wood by the application of animal proteins
2005 - IRG/WP 05-30387
The investigations consisted in the estimation of the effectiveness of protein borates and protein propionates in wood preservation against Coniophora puteana. The estimation was made on the basis of mass losses caused by the test fungus action against treated wood which was subjected to leaching with water. The investigations were performed for Scots pine wood (Pinus sylvestris L.) treated with p...
B Mazela, I Ratajczak, M Bartkowiak
Suppression of termite feeding by Sudan red 7B
1988 - IRG/WP 1344
Groups of the Formosan subterranean termite, Coptotermes formosanus Shiraki, were fed for 14 days on wood shavings soaked in acetone solutions of one of three concentrations (0, 0.5, or 1%) of the dye, Sudan Red 7B. Groups of marked or unmarked termites were then placed in containers and allowed to feed on undyed wood for 21 days. Dyed termites had lower wood feeding rate, survivorship, and number...
K S Delaplane, L A Bourg, J P La Fage
Characterization and differentiation of wood rotting fungi by protein and enzyme patterns
1999 - IRG/WP 99-20177
Standardized tests for wood preservatives are performed with defined fungal strains to ensure comparability between laboratories. However, changes of virulence and variation of results are well known events. Suitable and reliable measures to control the stability of the test organisms are necessary.Comparison of protein patterns produced by SDS-electrophoresis was already described by several auth...
U Schoknecht
Development of markers to predict decay durability of heat treated wood
2010 - IRG/WP 10-40496
Effect of heat treatment temperature on elemental composition of Scots pine sapwood (Pinus sylvestris) has been investigated in the range of temperatures between 220 and 250°C. Results revealed an important increase of carbon content, while oxygen content significantly decreases. Independently of the heat treatment temperature, elemental composition is strongly correlated with the mass losses due...
Ž Šušteršic, A Mohareb, M Chaouch, M Pétrissans, M Petric, P Gérardin