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Estimation of oral toxicity of boron as a bait toxicant and the trophallactic effects between individual members of termite colonies.
2003 - IRG/WP 03-10495
In recent years, because of the favourable environmental characteristics of boron, researchers in the wood preservation industries have refocussed on the use of boron as a major wood preservative against wood-destroying insects. Currently the greatest use of boron compounds is in remedial treatments. Boron has been found to have slow-acting toxicity against subterranean termites. Because of thi...
B M Ahmed


Protection of southern pine using N,N-Napthaloylhydroxylamine: Field tests, soft-rot cellars and aquatic bioassay leach testing
1999 - IRG/WP 99-30204
Recent environmental restrictions are limiting the use of broad-spectrum biocides for wood preservation. There is an urgent need for new, sharply targeted, environmentally benign wood preservatives. N'N-Napthaloylhydroxylamine (NHA), a water-soluble calcium-precipitating agent, has been shown to inhibit decay by brown-rot and white-rot fungi in soil-block tests and prevent damage by Easte...
D M Crawford, F Green III


International collaborative laboratory comparison of two wood preservatives against subterranean termites: Update
1994 - IRG/WP 94-20032
It was agreed by members at the termite workshop at the 24th annual meeting of IRG in Orlando, USA, in May 1993 to initiate an international subterranean termite laboratory bioassay to compare the various preferred termite protocols used by IRG termitologists. The author was nominated to co-ordinate this comparative laboratory evaluation of two wood preservatives (CCA and Cu-naphthenate) against t...
J R J French


Toxic value estimation of wood preservatives by using the probit analysis
1990 - IRG/WP 2348
As it was assumed from previous experiments, the probit analysis appears to be the most suitable method of all used to estimate the toxic value of wood preservatives. With the help of the classical agar-block method the probit analysis was tested for its suitability in: 1) fungitoxic value estimation of the WR-3 preservative (quaternary ammonium compound + borate), as compared to different test fu...
J Wazny, K J Krajewski


The metabolism and comparative elimination of polychlorinated biphenyl congeners in termites
1993 - IRG/WP 93-10038
Termites may serve as a potential supplementary food source for fish, poultry and pigs. Waste paper may be used as a source of food in mass rearing the termites. However, paper products and printing inks contain trace levels of toxic xenobiotics e.g. polychlorinatecl biphenyls (PCBs) and dioxins. This study examined the ability of Mastotermes darwinensis and Coptotermes acinaciformis to metabolise...
V S Haritos, J R J French, J T Ahokas


Laboratory evaluation of the termiticidal effectiveness of TanalithÃ’ 3485
1995 - IRG/WP 95-10109
The termiticidal effectiveness of the copper azole TANALITH 3485 was evaluated with the benchmark preservative TANALITH C in a laboratory bioassay using two species of subterranean termites, Mastotermes darwiniensis Froggatt and Coptotermes acinaciformis (Froggatt). Retentions of TANALITH 3485 tested were 0.15, 0.24, 0.285 and 0.40% m/m Cu and for TANALITH C 0.025, 0.05, 0.08 and 0.095% m/m Cu. Un...
J W Creffield, J A Drysdale, N Chew, N-K Nguyen


A bioassay for appraising preservative protection of wood above ground
1978 - IRG/WP 2124
A bioassay, using the mold fungus Aspergillus niger, gave results that were correlated with amounts of pentachlorophenol and tributyltin oxide in pine sapwood treated with solutions ranging in strength from 0.016 or below to 5%. Limited bioassay estimates of penta in commercially treated millwork corresponded to estimates by lime ignition. The assay fungus exhibited somewhat greater tolerance of t...
T C Scheffer, L Gollob


A bioassay to determine preservative retention in hardwoods and southern pines
1982 - IRG/WP 2183
In this paper the author discusses and suggests a new method of evaluating preservative retention in treated woods by means of bioassays. Twelve North American species were tested with different retention levels of pentachlorophenol (PCP) and copper/chrome/arsenic (CCA) preservatives. The results observed in this work show that among other fungi, Aspergillus niger responds to both preservatives, w...
J C Moreschi


International collaborative laboratory comparison of two wood preservatives against subterranean termites: Second update
1995 - IRG/WP 95-10117
As was agreed by members at the termite workshop at the 24th annual meeting of IRG in Orlando, USA, in May 1993 to initiate an international subterranean termite laboratory bioassay to compare the various preferred termite protocols used by IRG termitologists was initiated. The author was nominated to co-ordinate this comparative laboratory evaluation of two wood preservatives (CCA and Cu-naphthen...
J R J French


A study on the effectiveness of didecyldimethylammonium chloride to protect wood from attack by termites
1993 - IRG/WP 93-30009
A laboratory bioassay was conducted on the ability of didecyldimethylammonium chloride (DDAC) to protect Pinus radiata D.Don wood specimens from attack by two of Australia's most economically important species of subterranean termite, Mastotermes darwiniensis Froggatt and Coptotermes acinaciformis (Froggatt). Sapwood specimens of Pinus radiata, treated to achieve nominal retentions of 0.5...
J W Creffield


A laboratory bioassay method for testing preservatives against the marine borers Limnoria tripunctata, L. quadripunctata (Crustacea) and Lyrodus pedicellatus (Mollusca)
1990 - IRG/WP 4159
A laboratory culture and bioassay method is described for the marine borers Limnoria tripunctata, Limnoria quadripunctata, and Lyrodus pedicellatus. The methods were tested in a bioassay using established marine preservatives. The attack produced on blocks treated with CCA or creosote in some ways paralleled the attack found in the sea. Limnoria tripunctata attacked treated and untreated pine bloc...
L J Cookson


Determination of fungitoxic value of preservatives in laboratory wood-block tests. Part 2: Statistical estimation
1989 - IRG/WP 2327
Laboratory modified agar-block tests were made to determine the fungitoxic value of the wood preservative CCA against the test fungus Serpula lacrymans using statistical methods. With the aid of statistical computer programmes based on the least square method. the regression equation was found for the dependence between sample mass loss and preservative retention both in terms of percentage and fo...
J Wazny, J D Thornton, K J Krajewski


Determination of fungitoxic value of preservatives in laboratory wood-block tests. Part 1: Standard procedures
1989 - IRG/WP 2326
By applying a modified agar-block method, a comparative analysis was made on the toxic value of the wood preservative CCA against the test fungus Serpula lacrymans. The procedure applied to determine the results accounted for different standards: EN (Toxic limit), ASTM (threshold retention), GOST (threshold retention and protection probability), PN (toxic doses) and JIS (value of efficiency). Desp...
J Wazny, J D Thornton


Specifying preservative-treated resistant timber: Conforming to European Standards
2001 - IRG/WP 01-20235
The most common construction timbers used in the UK are of low natural durability and, generally, resistant to preservative treatment. These include species of spruce, hemlock and fir. However, their characteristically limited and non-uniform uptake of preservatives may still confer sufficient protection to give satisfactory durability performance in terms of biological resistance. This paper desc...
E D Suttie, A F Bravery, T B Dearling


Inter-laboratory comparison of assessment methods for wood preservatives against subterranean termites: Determination of protection threshold limits for CCA
1999 - IRG/WP 99-10317
In 1996, several institutes conducted laboratory bioassays on the efficacy of unleached Pinus radiata specimens treated with copper-chromium-arsenic (CCA) and copper naphthenate (Cu Naph) at retentions of 0.5, 1.0, 2.0 and 4.0 kg/m3 (total salt for CCA; elemental copper for Cu Naph) against a range of species of subterranean termites (IRG/WP/96-10174). Each participant employed the standard test m...
J W Creffield, M Lenz


A comparison of fungal strains used in the bioassay of wood preservatives
1984 - IRG/WP 2220
Previously published data are presented relating to a number of strains of wood-destroying basidiomycetes (Coniophora puteana, Coriolus versicolor, Gloeophyllum abietinum, Gloeophyllum sepiarium, Gloeophyllum trabeum, Lentinus lepideus, Poria placenta, Fibroporia vaillantii and Serpula lacrymans) commonly used as test fungi in the bioassay of wood preservatives. The data, which has not been statis...
J Wazny, H Greaves


Determination of toxicity data for preservatives against Basidiomycetes by measuring the reduction in compression strength of wood
1988 - IRG/WP 2297
Blocks of Pinus radiata D. Don were treated to 12 retention levels with CCA and NaPCP. These were exposed for 12 weeks in agar jars to the dry rot (brown rot) Serpula lacrymans (Schum. ex Fr.) S.F. Gray. After mass loss determination, the same blocks were tested for reduction in compression strength parallel to the grain. Using both these parameters, together with interpolated 6 week strength loss...
J Wazny, J D Thornton


A bioassay of remedially treated pole material
1987 - IRG/WP 3453
Stubs prepared from treated and untreated utility poles were installed in a test plot and pressure spade treated at the groundline with one of four preservative greases. At one and three year intervals, core samples were removed from the stubs and the improvement in the preservative efficacy due to the greases was assessed. The bioassay investigation showed that only bis(tributyltin) oxide (TBTO) ...
J N R Ruddick, L Parker


A comparison analysis of eight strains of Serpula lacrymans (Schum. ex Fr.) S.F. Gray
1991 - IRG/WP 2362
Investigations were previously carried out to compare eight strains of Serpula lacrymans (Schum. ex Fr.) S.F. Gray (some used in various countries as standard test strains): FPRL 12C (England), FPRL 12E (Germany), Warsaw III (Poland), HFP 7802 (Japan), DFP 16508, 16509, 16521 and 16522 (Australia). Studies included growth rate and dry mass of mycelium, decay capacity, reduction of compression stre...
J Wazny, J D Thornton


Antifungal activity in metabolites from Streptomyces rimosus
1991 - IRG/WP 1495
The objective of this study was to evaluate the efficacy of antifungal metabolites from Streptomyces rimosus for controlling the growth of sapwood-inhabiting fungi: sapstain fungi - Ceratocystis coerulescens, Ceratocystis minor, Ceratocystis pilifera, and Aureobasidum pullulans; mold fungi - Aspergillus niger, Penicillium spp, and Trichoderma spp. Production of antifungal metabolites by Streptomyc...
S C Croan, T L Highley


Boracol 40 - A potential remedial and preservative treatment for lyctids
1983 - IRG/WP 1192
The paper reports on a preliminary assessment of the efficacy of a diffusion formulation, Boracol 40, when applied as a brush treatment: to the surface of Eucalyptus obliqua against the powder-post borer Lyctus brunneus. After a five month bioassay, Boracol 40 has severely retarded the progress of active infestations of Lyctus brunneus and has been 100 per cent effective as a preventative treatmen...
J W Creffield, H Greaves, C D Howick


In vitro antifungal activity of chilli against wood degrading fungi
2006 - IRG/WP 06-10572
The efficacy of chilli juice and/or chilli extract oleoresin as antisapstain agents was evaluated against two common sapstain fungi, Sphaeropsis sapinea and Leptographium procerum. Possible synergy between chilli juice and Lactobacillus casei as antisapstain agents was also assessed. Both the chilli juice and the oleoresin showed moderate antifungal activity. No growth of the test fungi was obse...
T Singh, C Chittenden, D Vesentini


Laboratory bioassay and field trial on imidacloprid and cypermethrin as glueline treatments for softwood plywood
2006 - IRG/WP 06-30405
The effectiveness of imidacloprid and cypermethrin as glueline treatments for protecting phenol formaldehyde (PF)-bonded Pinus radiata (radiata pine) plywood from attack by subterranean termites was evaluated both in the laboratory and field. Imidacloprid was evaluated in two plywood constructions (19 ply x 1.6 mm-thick veneers and 5 ply x 3.2 mm-thick veneers) whereas cypermethrin was evaluated i...
J W Creffield, D K Scown


Methods for Determining the Role of Extractives in the Natural Durability of Western Redcedar Heartwood
2007 - IRG/WP 07-20356
The durability of western redcedar heartwood is influenced by a number of different extractives operating by different mechanisms. In order to guide a systematic isolation of extractives with high fungal toxicity, a micro-bioassay has been developed for measuring the fungal toxicity of extractive fractions. Two additional assays quantify the anti-radical and metal chelating properties of cedar ext...
R Stirling, C R Daniels, J E Clark, P I Morris


Antifungal activity of plant derived extracts against G. trabeum
2007 - IRG/WP 07-30433
While synthetic chemicals have provided excellent protection to woods used in adverse environments, the general public remains interested in naturally derived wood protectants. There are diverse arrays of possible candidates, but many of these compounds are not readily water soluble and efforts to render them soluble often reduce biological activity. In this report, we describe efforts to enhance ...
M Maoz, I Weitz, M Blumenfeld, C Freitag, J J Morrell


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