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Use of Confocal Profilometry to Quantify the Weathering of Wood
2009 - IRG/WP 09-20421
The methods that have been used to screen chemicals for their ability to prevent the weathering of wood involve assessing the loss in weight and tensile strength of treated wood veneers or measuring the rate of erosion of exposed and unexposed (masked) wood subjected to weathering. The erosion of wood during natural weathering occurs slowly and long exposure times are required to produce differenc...
Chunling Liu, P Evans


Confocal laser scanning microscopy of a novel decay in preservative treated radiata pine in wet acidic soils
1997 - IRG/WP 97-10215
Light microscopy of radiata pine (Pinus radiata D. Don) field test stakes (20x20x500mm3) exposed in wet acidic (pH 3-4) soil for 12 - 24 months showed predominance of an unusual type of decay characte-rised by tunnelling attack of wood cell walls. After two years decay was moderate to severe in wood treated to ground contact CCA specifications and also equivalent retentions of creosote, and a numb...
R N Wakeling, Ying Xiao, A P Singh


The use of immunofluorescence labelling for detecting Ophiostoma piceae in radiata pine
1998 - IRG/WP 98-10268
The primary objective of the current investigation is to understand fungal interactions of dominant sapstaining fungi in radiata pine using confocal laser scanning microscopy (CLSM). In the current paper, an immunofluorescence labelling technique was developed for detection of Ophiostoma piceae using a monoclonal antibody. The primary antibody was labelled with Oregon green 514 Goat anti-mouse Ig...
Ying Xiao, B Kreber, C Breuil


Bordered Pit Imaging
2012 - IRG/WP 12-10773
New findings about bordered pits will be presented using the latest microscopy techniques. Three-dimensional imagery at the nanolevel is used, and short 3-D movies will shown as part of the discussion on this topic to reveal new features that have not previously been reported in pits. The implications for both microorganism penetration through lignified cells as well as preservative penetration i...
D Mascheck, B Goodell, H Militz, M Lessard, J Jellison


Confocal Laser Scanning Microscopy (CLSM) of decayed wood
1998 - IRG/WP 98-10273
Confocal laser scanning microscopy (CLSM) is currently being used to examine biodegraded wood and is proving to be a useful new technique in this area of work. Non-invasive optical sectioning within a thick specimen (20 - 50 µm), coupled with post image processing techniques allows manipulation of images and 3-D reconstruction from serial sections. Glutaraldehyde can cause cell autofluorescence,...
Ying Xiao, R N Wakeling, A P Singh


Visualising Bacteria in Wood Using Confocal Laser Scanning Microscopy (CLSM)
1998 - IRG/WP 98-10272
A fluorescent phospholipid probe was used in conjunction with confocal laser scanning microscopy (CLSM), to visualise bacteria which inhabit in radiata pine wood and degrade pit membranes. CLSM has the ability to collect fluorescent images through different emission filters at the same time, so it is possible to distinguish gram-positive and gram-negative bacteria in infected wood by counterstaini...
Ying Xiao, A P Singh, R N Wakeling


Analyzing hyphal growth of the bio incising fungus Physisporinus vitreus with light-, confocal laser scanning- and, Synchrotron X-ray tomographic microscopy
2010 - IRG/WP 10-20438
Norway spruce wood is an important building material for the forest and wood industry. To improve wood permeability and uptake of wood modification substances Physisporinus vitreus is employed as a bioincising agent. In this study wood specimens were incubated with P. vitreus to assess hyphal colonisation and structural alterations of Norway spruce wood. After different incubation periods, semi-th...
C Stührk, M Fuhr, M Schubert, F W M R Schwarze