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Detection of increased metal cations after wood decay using Chromeazurol-S
1997 - IRG/WP 97-20112
Chromeazurol-S (CAS) is a dye used for the spectrophotometric determination of metals, mainly aluminum (Al), berillium (Be) and copper (Cu). CAS has been widely used for determining the penetration of copper containing preservatives like CCA (AWPA A3-96). Additional uses include: i) detection of utility pole decay (Esyln, 1979), ii) a chemical spot test for aluminum in wood (Kukachka and Miller, 1...
F Green III, U Srinivasan, R B Miller


Use of immunoblotting for the analysis of wood decay basidiomycetes
1988 - IRG/WP 2307
Immunoblotting methods, in particular dot and Western blotting have been used to investigate features of a variety of wood decay organisms, in particular Coriolus versicolor, Lentinus lepideus and Serpula lacrymans. Antisera to each of these decay organisms has been produced by immunisation of rabbits with liquid culture grown hyphae. These antisera, after appropriate preabsorption with sawdust ha...
J W Palfreyman, H Glancy, D Button, A Vigrow, A J Score, B King


The use of fumigants for controlling decay of wood: a review of their efficacy and safety
1989 - IRG/WP 3525
Volatile agricultural chemicals (fumigants) such as chloropicrin (trichloronitromethane) and sodium n-methyldithiocarbamate have been used in the United States for controlling internal decay of large dimension wood products for over 20 years. This usage has been concentrated in the electric utility industry, but fumigants are increasingly applied to protect bridge timbers, marine piling, and even ...
J J Morrell


Immunolabelling studies on the detection of enzymes during the degradation of wood by Phanerochaete chrysosporium
1988 - IRG/WP 1364
The degradation of lignin in native lignocellulosic substrates by white rot fungi is poorly understood. Biochemical studies have shown the involvement of a number extracellular ligninolytic enzymes released by white rot fungi which are capable of the oxidative conversion of DHP's (lignin model compounds) in vitro, but to date conclusive evidence for occurrence of these enzymes in wood und...
G F Daniel, T Nilsson, B Pettersson


Detecting fungal DNA in treated and non-treated wood
2007 - IRG/WP 07-10621
Isolating fungi from wood has long involved culturing on selective media followed by identification using various keys. This process can be cumbersome, costly, and, most importantly, not always capable of detecting all of the fungi present. The recent development of molecular methods for isolation and identification of fungi has created tremendous opportunities for expanding our knowledge of the...
C Freitag, M Freitag, J Morrell


Early detection of colonisation by blue stain and mould on modified wood using PCR technique
2010 - IRG/WP 10-10730
The aim of this research was the early detection of fungal attack on the surfaces of modified and unmodified wood after natural weathering. The investigation was carried out by molecular techniques and the development and establishment of genus/species specific PCR for important moulds and sapstain fungi was part of this work. After adaption of the method, a monitoring of the succession of Ascomyc...
A Steitz, B Schmöllerl, N Pfabigan, S Bollmus, G Grüll


Measurements of rot fungal activity as a function of moisture content by isothermal calorimetry
2010 - IRG/WP 10-20428
Measurements of heat production rate have been made on wood samples with the brown rot fungus Postia placenta at different moisture contents. The results clearly indicate that the heat production rate (a measure of respiration rate and activity) is moisture dependent. When the moisture content is decreased, less heat is produced, and when the moisture content is increased, more heat is produced. I...
L Wadsö, A Pilgård, G Alfredsen


Detection of Wood Destroying Fungi Using DNA Microarray Technology
2010 - IRG/WP 10-20435
Wood decay fungi of the phylum Basidiomycota cause serious damage to wooden constructions and building elements. The elimination and the appraisement of fungal decay require an assured species identification. Conventional fungal diagnostics are mainly based on morphological characteristics by macro- and microscopy analysis. For some years, standardised and highly sensitive molecular methods focuss...
K Jacobs, N Rangno, W Scheiding, B Weiss, D Müller, C Hiller, W Brabetz


A critical view on early indicators for above ground field performance of wood
2013 - IRG/WP 13-20509
Above ground field tests are quite often a balancing act between the provision of realistic test conditions, reliable statistics, and acceptable long test durations. Within this study we therefore reviewed 543 data sets from above ground field tests performed at 36 different test sites in 8 countries in Australia and Europe. The main objective was to investigate possible correlations between early...
C Brischke, L Meyer, G Alfredsen, P-O Flæte, L Francis, M Hansson, P Larsson Brelid, J Jermer


The activity of a wood-decaying fungus during drying and rewetting cycles measured by isothermal calorimetry
2013 - IRG/WP 13-20526
Wood decaying fungi are an essential part of any ecosystems as they are the main decomposers of cellulose and lignin-containing materials. But as wood is a common building material the risk for decay fungi growth and subsequent degradation of our construction material is a concern. There are important physiological aspects of the growth and activity of decay fungi that are unknown today, and witho...
S Johansson, L Wadsö, A Pilgård, G Alfredsen


What molecular biology can tell us about the biodegradation of lignocellulose: the utilization of molecular techniques for the detection, identification and enhanced understanding of wood degrading organisms
2013 - IRG/WP 13-20528
Molecular techniques are now routinely used in the identification, detection and analysis of wood degrading organisms. An overview of some of the early work on nucleic acid isolation and characterization will be followed by a discussion of the power of sequencing and other procedures for better understanding: the mechanisms involved in the biological degradation of wood, the metabolic basis of pre...
J Jellison, B Goodell, G Alfredsen, D Eastwood, G Daniel, S M Cragg, J K Grace


Real-Time PCR Assays for the detection of the dry rot fungus Serpula lacrymans and its close relative Serpula himantioides
2016 - IRG/WP 16-10867
Prevention of wood decay by fungi of the phylum Basidiomycota is an important issue of wood protection. The most destructive fungus in buildings in Central Europe is the dry rot fungus Serpula lacrymans, which needs more extensive restauration measures than all other wood decay fungi. Its close relative S. himantioides occurs more frequently in outdoor environment. The assessment and remediation o...
K Jacobs, N Rangno


Effects of exposure time on detection of the behavior of white-rot fungi on Oriental beech wood properties
2017 - IRG/WP 17-10902
The comparison of fungal degradation of Orientalis beechwood by two white-rot fungi was evaluated in this study. Biological, mechanical, and chemical assessments of beech wood were carried out at 30 day interval for 120 days. The results demonstrated that both fungi have greater effects on beech wood during each exposure time. However, Trametes versicolor degraded beech wood more effectively than ...
E Bari, D D Nicholas, K M Ohno


Morphological observation of wood at the early stages of decay in brown rot and white rot
2023 - IRG/WP 23-11020
Wood rotting fungi, the fungal species causing biodeterioration for wood building, are generally classified into white-rot, brown-rot and soft-rot fungi based on their decay modes. Since white-rot and brown-rot fungi are known to reduce wood strength significantly, it is important to clarify the mechanisms of their wood degradation. White-rot fungi reduce wood strength as the decay progress and de...
R Tsukida, T Hatano, Y Kojima, Y Horikawa, S Nakaba, R Funada, M Yoshida


Confocal laser scanning microscopy of a novel decay in preservative treated radiata pine in wet acidic soils
1997 - IRG/WP 97-10215
Light microscopy of radiata pine (Pinus radiata D. Don) field test stakes (20x20x500mm3) exposed in wet acidic (pH 3-4) soil for 12 - 24 months showed predominance of an unusual type of decay characte-rised by tunnelling attack of wood cell walls. After two years decay was moderate to severe in wood treated to ground contact CCA specifications and also equivalent retentions of creosote, and a numb...
R N Wakeling, Ying Xiao, A P Singh


Effect of acetylation on decay resistance of wood against brown-rot, white-rot and soft-rot fungi
1989 - IRG/WP 3540
Effect of acetylation on decay resistance of wood was investigated using wood blocks of Cryptomeria japonica, Pinus densiflora, Albizia falcata and Fagus crenata. Blocks were treated with uncatalyzed acetic anhydride for different lengths of time and exposed to Tyromyces palustris, Serpula lacrymans, Coriolus versicolor and unsterilized soil. The action of OH-radical on acetylated wood was also ex...
M Takahashi, Y Imamura, M Tanahashi


Field trial with poles of Scots pine treated with six different creosotes
1996 - IRG/WP 96-30115
In the middle of the 50's field trials with creosote-treated poles were started in France, Germany and Sweden. The trials were initiated by WEI (Western-European Institute for Wood Preservation). Six different creosotes were used and 40 poles per creosote were installed at each test field. Results after 39 years of exposure in Simlangsdalen, Sweden are reported. Poles treated with a heavy...
Ö Bergman


Questionnaire - Fungal decay types
1985 - IRG/WP 1265
T Nilsson


JWPA method for testing effectiveness of surface coatings with preservatives against decay fungi
1981 - IRG/WP 2164
In 1979 JWPA established a new method for testing effectiveness of surface coatings in accordance with practical use of preservative-treated lumber. Comparing the new testing method with JIS A 9302, a few new trials - size of wood specimen, weathering procedure, and decay-test procedure - are incorporated....
K Tsunoda


Co-operative studies on determining toxic values against wood-destroying Basidiomycetes: Progress report to May 1989
1989 - IRG/WP 2339
This document reports progress on the co-operative study between nine laboratories set up following the proposals contained in Document IRG/WP/2316. Results have been received from two laboratories. Toxic values data have been established successfully using the test fungus Coniophora puteana but problems have been encountered with the other test fungi....
A F Bravery, J K Carey


Monographic cards for wood-destroying fungi. [Fiches monographiques pour les champignons lignivores]
1970 - IRG/WP I 5B
C Jacquiot


On Donkioporia expansa (Desm.) Kotl. & Pouzar
1986 - IRG/WP 1285
Donkioporia expansa is found more often in houses than realised until now. Virulence tests according to EN 113 show not only an attack of oak, but also of other hardwoods and even soft-woods....
G Buchwald


Nondestructive Evaluation of Oriented Strand Board Exposed to Decay Fungi
2002 - IRG/WP 02-20243
Stress wave nondestructive evaluation (NDE) technologies are being used in our laboratory to evaluate the performance properties of engineered wood. These techniques have proven useful in the inspection of timber structures to locate internal voids and decayed or deteriorated areas in large timbers. But no information exists concerning NDE and important properties of wood composites exposed to dec...
B Illman, V W Yang, R J Ross, W J Nelson


Moisture content levels and decay of hemlock
1986 - IRG/WP 1287
As a model of decay conditions of wooden members in wooden houses, a decay test was set up in which samples of western hemlock (Tsuga heterophylla) under 4 moisture levels were examined. Each week the samples were weighed and if the weights indicated that their moisture contents were lower than the expected levels, distilled water was added. Every 8 weeks 3 samples from each condition were oven dr...
K Suzuki


Dimensional stability and decay resistance of hot-melt self-bonded particleboard by surface benzylated pine chips
1991 - IRG/WP 3652
Akamatsu (Pinus densiflora Sieb. et Zucc: Japanese red pine) particles were pretreated with 40% NaOH solution and benzylated with benzyl chloride, and the surface of particle was converted into meltable materials. Hot-melt self bonded particleboard having smooth and high glossiness surface was prepared by hot pressing at 150°C and 1.96 MPa without using any conventional adhesives. Dimensional sta...
M Kiguchi, K Yamamoto


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