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Bioactivity of Eucalyptus camaldulensis essential oil against Microcerotermes diversus (Isoptera: Termitidae)
2013 - IRG/WP 13-30631
Microcerotermes diversus Silvestri (Isoptera: Termitidae) is the most economically destructive wood pest in structures in Khuzestan province (Iran). Chemicals such as essential oils and plant extracts that are compatible with the environment and that have high potential to be used in integrated pest management programs are extremely important. This study evaluated the repellency, contact and digestive toxicity of Eucalyptus essential oil in no-choice and choice bioassays and feeding inhibition trials on M. diversus. Concentrations of the essential oil ranged from 0.3 to 1.6 % (g cc-1). The results of the choice tests and feeding inhibition trial showed that the essential oil could act as a repellent at the given concentrations. Concentrations used in these tests resulted in mortality of termites, and a direct relationship between concentration and mortality was observed. The essential oil also increased the mortality of termites at concentrations higher than 0.7%. Termite feeding decreased with increased in concentration. Due to the ability of termites to choose the untreated filter-paper in the choice trial, values of LT and LC, were higher than in no-choice trials. The highest effects of Eucalyptus essential oil (≈100% mortality) was induced by the concentration 1.6%. Overall, this study reveals that Eucalyptus essential oil may be suggested as an effective toxicant with suitable contact and digestive toxicity and repellent effects on M. diversus.
B Habibpour, E Shafiei Alavijeh, A Rasekh

Field test evaluation of preservatives and treatment methods for fence posts
1985 - IRG/WP 3347
This work presents the field test results after fifteen years exposure of Eucalyptus saligna fence posts treated with six different preservatives and five treatment methods. All the combinations with oil-borne preservatives presented the best results and among the waterborne preservatives, the fence posts treated by immersion method were with the lowest performance in the field test.
G A C Lopez, E S Lepage

Effects of some essential oils on wood destroying organisms
1993 - IRG/WP 93-10047
Three wood destroying fungi: Botryodiplodia theobromae Pat. (stain), Gloeophyllum sepiarium (brown rot), and Pycnoporus sanguineus (white rot) were exposed to six plant essential oils: the peppermint, kaffir lime or leech lime, lavender, tarragon, holy basil, and the eucalyptus. The peppermint oil showed most effective to inhibit fungal growth, while eucalypus oil was the least effective. The other oils inhibition rate varied according to the species of fungi. In the experiment of the powder post beetles Heterobostrychus aequalis Waterh., the insects were killed within three days in the oil of tarragon, eucalyptus and holy basil, while in lavender oil they could live to ten days the same as controls. But on the contrary in the oil of peppermint and kaffir lime, some of them could even lived longer than the controls.
K Atisongkroh, C Anantachoke, P Lekuthai, S Pensook, T Kittirattrakarn

The evaluation of the effectiveness of wood preservatives by means of IUFRO's method for field tests with wooden stakes
1985 - IRG/WP 3348
Pinus elliottii and Eucalyptus saligna stakes treated with CCA-A, CCA-C, CCB, Cashew Nut Shell Oil and Benzotar solutions were exposed in seven test sites in Sao Paulo State, Brazil. After five years of exposure, between oil-borne preservatives Benzotar show better performance than CNSL (cashew nut shell liquid), and within waterborne preservatives the performance of CCB is not so good as that of CCA-A and CCA-C, which show similar performance.
G A C Lopez, A M F Oliveira, E S Lepage

Soft rot in heartwood of preservative-treated pole stubs of Eucalyptus cypellocarpa L. Johnson
1983 - IRG/WP 1204
Pole stubs of Eucalyptus cypellocarpa L. Johnson, treated in the sapwood with certain waterborne preservatives and exposed for 21 to 23 years in a dry tropical region were severely soft-rotted in the heartwood, as well as the sapwood. They broke with a brash fracture when stress tested (loaded as a cantilever) in the field. The severe soft rot in the heartwood was not detected by the knife test or the Shigometer. Similar stubs, treated with oilborne preservatives, showed no microscopic evidence of soft rot in the heartwood and the few which have broken under load did so with a tension-type fracture. Implications of these findings for future testing of soft rot susceptibility of eucalypt heartwood within a treated annulus are discussed.
J E Barnacle, G C Johnson, M A Tighe

Termite Response to Oil-Heat-Treated Norway spruce, Scots Pine and Eucalyptus Wood
2006 - IRG/WP 06-20325
The work here presented focused on the behaviour of subterranean termites (Reticulitermes grassei Clément) towards Oil-Heat-Treated (OHT) Norway spruce (Picea abies L.), Scots pine (Pinus sylvestris L.) and eucalyptus (Eucalyptus globulus Labill) wood, with a particular interest in the development of simple methods adequate to study the resistence of these materials to termite attack. Small test specimens (30 x 10 x 10 mm) were exposed to the termites in a no-choice test adapted from the European Standard EN117 and in, developed for the purpose, choice and repellence tests, with maritime pine (Pinus pinaster Ait.) as virulence control in all tests. The OHT Norway spruce, Scots pine and eucalyptus wood proved to be non-toxic and non-repellent to termites but exhibit an increase in durability when higher oil retentions were achieved, as in the case of Scots pine treatments.
L Nunes, T Nobre, C Welzbacher, A O Rapp

Moldicidal properties of seven essential oils
2006 - IRG/WP 06-30404
When wood and wood products are exposed to moisture during storage, construction or while in-service, mold growth can occur in 24 to 48 hours. Mold growth could be suppressed or prevented if wood was treated with an effective mold inhibitor. The objective of this study was to evaluate the mold inhibiting properties of natural plant extracts such as essential oils. Seven essential oils were evaluated for their ability to inhibit growth of Aspergillus niger, Trichoderma viride, and Penicillium chrysogenum on Southern yellow pine (SYP) specimens that were either dip-treated or exposed to volatiles of the test oils. Dip treatment with thyme or geranium (Egyptian) oil inhibited growth of test fungi for 20 weeks. Vapors from dill weed oil also inhibited all test fungi for at least 20 weeks when the vapor source remained in the test apparatus. Essential oils may be useful as moldicidal surface-treatments or fumigants for wood and wood products.
V W Yang, C A Clausen

Potentiality of use extracts from Tetraclinis articulata like biocide against wood destroying organisms: Reticulitermes santonensis
2007 - IRG/WP 07-30418
Screening trials were conducted to determine the antitermitic and antifungal properties of essential oil and methanolic extractives obtained from Tetraclinis articulata heartwood. Extract-treated cellulose pads were used to evaluate antitermitic activity and complete termite mortality was obtained with the essential oil: the threshold is situated under 5% (v/v). Standardised tests according European standard EN 118 allow validate the use of essential oil like biocide.
F El hanbali, N Amusant, F Mellouki, M Akssira, C Baudasse

Antifungal Essential Oil Metabolites
2010 - IRG/WP 10-30531
New environmentally-friendly wood protection systems based on “green” technologies are needed to inhibit wood-inhabiting mold and decay fungi. Utilizing bioactive essential oils from select herbaceous plants is one promising approach, but the concentrations of bioactive compounds are somewhat variable even in the highest (therapeutic) grade essential oils. Purified primary metabolites from four bioactive plant essential oils were evaluated for antifungal activity in southern pine treated with those compounds. Purified carvone, citronellol, geraniol, thymol and borneol inhibited growth of Aspergillus niger, Penicillium chrysogenum and Trichoderma viride for 12 weeks at concentrations equal to or less than those present in therapeutic grade essential oils. Thymol and borneol effectively inhibited two brown-rot fungi, Postia placenta and Gloeophyllum trabeum and one white-rot fungus, Trametes versicolor, but other metabolites tested were ineffective against the decay fungi. Select purified bioactive metabolites of essential oils effectively inhibit fungi that inhabit wood and wood products.
C A Clausen, B M Woodward, V W Yang

Natural compounds: A review of their use for wood protection
2010 - IRG/WP 10-30545
A lot of research in the field of wood protection has focused on natural compounds but very few of these have been implemented by industry. This review is an attempt to bring together information from selected area of work: - A brief review of the work done on natural products as organic biocides for wood protection. - Table containing information on natural products, their use as organic biocides, references to studies done. - Brief discussion about possibilities and limitations of using natural compounds as wood protectant.
T Singh, A P Singh

Antifungal activity of wood extractives from waste products of steam distillation of Aniba rosaeodora
2012 - IRG/WP 12-10779
Aniba rosaeodora, is a slow growing evergreens of the Lauraceae family which are indigenous over a wide range of the Greater Amazon Region (the Guianas and Venezuela, Brazilian Amazon…). The essential oil obtained from the wood has a characteristic aroma and is a long-established ingredient in the more expensive perfumes. Around the olfactive characteristic of the essential oil is due to the presence of levogyre linalol. The steam distilled wood oil is obtained in a yield ranging around 1% and up to 90% of the oil consists of optically active linalol. This work is focused on the valorization of steam distillated sawdust, waste obtained from extraction of essential oil. After steam distillation, the sawdust was extracted by ethyl acetate and methanolic solvents and the antifungal activity was evaluated against basidomycetes fungi. The both extracts were active in vitro against white and brown rot fungi and allowed to propose these extractives as wood preservative agent.
N Amusant, A Digeon, E Hoüel, J Beauchène

Chemical compositions and anti-termite activities of essential oils from Gabonese Canarium schweinfurthii Engl, Dacryodes buettneri Engl and Aucoumea klaineana Pierre wood resins.
2017 - IRG/WP 17-10895
Essential oil extract from resins of Canarium schweinfurthii, Dacryodes buettneri and Aucoumea klaineana woods from Cap Esterias and Oyem areas, Gabon, were prepared by Clevenger - steam distillation. The chemical compositions of these respective essential oils were analyzed by a Gas Chromatography coupled with Mass Spectrometry (GC-MS). Although monoterpenes were the main constituents of the three essential oils, each wood resins had a various essential oil yield after steam distillation process [6,92% (A. klaineana), 4,20% (C. schweinfurthii) and 13,19% (D. buettneri)] and their respective chemical compositions were slightly different. It results that monoterpenes, as α –pinene, o-cymene, alpha-phellandrene and D-limonene form the major constituents of terpenoides and phenylpropanoïdes compounds which are the most active substances against termite activity. The anti-termite activities of the three essential oils were evaluated, performing no-choice tests. 70 μL of each essential oil diluted in acetone with mass ratios of 50:50 and 25:75 [essential oil: acetone] were impregnated on Whatman papers and exposed to termite (Reticulitermes flavipes). Essential oil from Canarium schweinfurthii resin showed the strongest inhibitory activity against the termite with 100% mortality after 1 day at 50:50 and 25:75 concentrations followed by Aucoumea klaineana resin with the 100% mortality after 3 days at 50:50 and 25:75 concentrations. Finally, essential oil from Dacryodes buettneri resin showed the lowest termite resistance with 48.34 % and 58.34% mortalities after 14 days at 50:50 and 25:75 concentrations, respectively. The number of chemical components from each essential oil and their respective quantity, determined by GC-MS, are related to their anti-termite activity level.
Chemical compositions and anti-termite activities of essential oils from Gabonese Canarium schweinfurthii Engl, Dacryodes buettneri Engl and Aucoumea klaineana Pierre wood resins.

Response of the symbiotic flagellate protists community of subterranean termites to sublethal amounts of biocides
2018 - IRG/WP 18-10911
Subterranean termites are quite efficient at extracting nutrients from lignocellulose. Their ability relies not only on the digestive tract physiology but also on symbiotic relationships established with flagellate protists and bacteria. This work aimed to screen the response of the flagellate protists community of the subterranean termite Reticulitermes grassei Clément to the ingestion of different biocides. The substances chosen were applied at sublethal doses and included antibiotics (amoxicillin), an antiprotozoal (metronidazole), a termite intestine pH alteration agent and respiration inhibitor (boric acid), an essential oil (cloves) and its main constituent (eugenol), together with the solvent (water) and a positive control of pine wood. Termites were captured in three different zones of the same pine forest, sufficiently distant to be considered as different colonies; three replicate samples from each colony were selected for testing. Immediately after termite capture the initial flagellate protists community was evaluated for all samples (initial controls). Groups of termite workers were then fed on diet disks impregnated with the substances and, after the trials the diversity and abundance of the flagellate protist community was evaluated. Twelve morphotypes were present in the controls. The naturally less abundant morphotypes were positively associated with the termites screened before the trials and the ones fed on water treated diet disks or original wood. Metronidazole showed to affect negatively most morphotypes, however, two morphotypes’ abundance increased; these two morphotypes abundances decreased when termites fed on amoxicillin treated diet disks. For eugenol and boric acid significant negative impact was found for one morphotype with parallel increase in abundance of two others. Overall, the results suggest a possible maintenance of hindgut equilibrium or minimum functioning relying both on: changes on abundances of two or three morphotypes; and presence and abundance of the less common morphotypes. Three morphotypes exhibit differentiated response to changes in hindgut conditions, triggered by the addition of substances to the termite diet. This dynamic nutritional symbiosis equilibrium seems to provide a wide range of defences of the termite to exposure to substances potentially harmful and general dietary changes.
S Duarte, T Nobre, P Borges, L Nunes

Chemical composition, antitermite and antifungal activity of Dacryodes edulis oleoresin
2019 - IRG/WP 19-20653
Damages to wood structures and other cellulosic materials caused by wood destroying insects and fungi are estimated to several billions of dollars each year in the world. Among these, termites are considered as one of the most economically important pests for wooden structures. In the past, several wood protection chemicals like CCA, creosote, lindane or pentachlorophenol have been used. However, even if some of these products are still in use depending of the countries and of their own regulations, most of them have been largely limited in Europe (and Northern America or even banned because of their impact on the environment and the human health. Growing environmental pressures associated to the decrease of fossil resources has contributed to significant changes in the field of wood preservation leading to the research of more environmentally acceptable wood preservation solutions. In this context, products issued from renewable biomass present several advantages: they require less energy to be produced limiting carbon dioxide emissions, biodegradability of biomass make them generally less harmful to the environment. The use of natural products derived from renewable raw materials, replacing chemicals of petrochemical origin, is therefore of growing interest. Some wood species are naturally resistant to termites and fungi attacks, due to the presence of secondary metabolites produced by trees as natural defense system. Dacryodes edulis, also known as African plum tree (En) or Safoutier (Fr), occurs naturally in Gabon, where it is widely used for its fruits. Its wood is reported to present similar properties to African mahogany, but it is still mainly used as firewood, even if it is reported by local populations to be resistant to termites. The tree is also able to exudate oleoresin in response to different stress or injuries. Indeed, exudation is a natural mechanism that plants use to heal their wounds. In addition, some authors consider it to be a protection in response to mechanical lesions or microbial invasion. In this context, the aim of this study was to evaluate the properties of Dacryodes edulis oleoresin as potential anti-termite and fungicide agents to develop more acceptable wood protection systems based on used of bio-pesticides. For this purpose, essential oil was separated from oleoresin using steam distillation with a Clevenger apparatus, while oleoresin was purified using different solvents. Each fraction was analyzed using GC-MS and subjected to different biological tests to evaluate their anti-termite and fungicidal properties.
W F Bedounguindzi, K Candelier, P E Engonga, Se Dumarcay, M-F Thevenon, P Gerardin

Efficiency of three resin fractions from Aucoumea Klaineana Pierre, Canarium schweinfurthii Engl and Dacryodes edulis (G. Don) H. J. Lam from Gabon combined with Tebuconazole as wood preservatives
2022 - IRG/WP 22-10998
Pure resins from Aucoumea Klaineana, Canarium schweinfurthii and Dacryodes edulis were harvested and then hydro-distilled to obtain essential oils and purified resins fractions. Due to the potential leaching and volatility hazards of the compounds that constitute these fractions, resins and essential oils were combined with tebuconazole to produce both antifungal and anti-termite wood preservatives formulations. Beech and scots pine wood block samples were impregnated by these different formulations and expose to Trametes versicolor and Coniophora puteana, respectively. Treated and control scots pine samples were also expose to termite (Reticulitermes flavipes). The results indicate that the use of aqueous formulations of the three fractions of the different Gabonese wood species combined to tebuconazole improves the wood resistance against termites and Coniophora puteana. Currently, the promising protection obtained would be limited to indoor applications since the different formulations should be formulated to be resistant to leaching.
W F Bedounguindzi, K Candelier, P Edou Engonga, S Dumarcay, M-F Thevenon, P Gerardin

IRG/COIPM INTERNATIONAL MARINE TEST - to determine the effect of timber substrate on the effectiveness of water-borne salt preservatives in sea-water. Progress Report 2: Report of treatment and installation in Australia
1978 - IRG/WP 440
The purpose of this test and the procedures to be followed have been fully set out in documents distributed by the International Research Group on Wood Preservation and numbered IRG/WP/414 and IRG/WP/420. The prescriptions set out in these two documents have been closely followed.
J Beesley

Fire resistance of preservative treated fence posts
1994 - IRG/WP 94-30033
Pine fence posts were pressure treated separately with CCA-C, CCA-wax, CCA-oil and creosote. Treated posts and untreated controls were planted in the ground in a randomised block design, weathered for six months and then subjected to a controlled burning test using two fuel loads. Creosote treatment increased the time that posts were alight whereas CCA treatment had no such effect. However, CCA treated posts smouldered until destruction of the majority of the posts occurred. Posts treated with CCA-oil took longer for destruction to occur than posts treated with CCA-C or CCA-wax. Creosote treated posts and untreated controls did not show prolonged smouldering and consequently were not destroyed by the burning test, although their strength was reduced. A high fuel load increased the time that posts were alight and smouldering, and for CCA treated posts decreased their time to destruction.
P D Evans, P J Beutel, C F Donnelly, R B Cunningham

Phoracantha semipunctata Fab. dans le sud-ouest Espagnol: Lutte et dommages
1985 - IRG/WP 1250
L G Tirado

Pinus and Eucalyptus fenceposts treated with creosote and solvex tar by hot and cold open-tank process
1987 - IRG/WP 3455
A comparative study of the behaviour of two different wood preservatives, creosote and solvex-tar, was made, using two wood species, Pinus pinaster Ait and Eucalyptus globulus Labill, by the hot and cold open-tank process. Results showed that the creosote behaved better in relation with the uniformity of its distribution in wood. On the other hand, better results were obtained on Pinus for both preservatives.
M V Baonza Merino

Effectiveness of "Gang-Nail" plates in preventing splitting of Eucalyptus poles and Brazilian hardwood sleepers
1984 - IRG/WP 3262
This paper presents the results of some tests carried out with an anti-splitting device, placed on the end surfaces of Eucalyptus spp utility poles and Brazilian hardwood sleepers at the beginning of an air-drying period. The type of device used, a "Gang-Nail" plate, reduces significantly the splits at the end-surface of poles, but reduces only a little the splits occurring in sleepers.
A M F Oliveira, J A C Sodré, O B Neto

Field trials of groundline remedial treatments on soft rot attacked CCA treated Eucalyptus poles
1983 - IRG/WP 3222
A total of 17 CCA treated Eucalyptus poles, which were found to contain 2-5 mm of soft rot in October, 1980, were reinspected in October, 1982. In 1980, 11 of the poles were given a supplemental groundline bandage treatment of either Osmoplastic or Patox, while 6 of the poles were designated as untreated controls. Two years after remedial treatment, samples were removed from the poles for microscopic observations and for chemical retention analysis. It was found that the remedial bandage treatments were effective in preventing any further advance of soft rot. Based on the positive results of this study, a treatment efficacy of five years or longer is predicted.
W S McNamara, R J Ziobro, J F Triana

Performance of preservative-treated hardwoods with particular reference to soft rot. Report of condition of specimens installed in Victoria, Australia
1980 - IRG/WP 3155
J Beesley, R McCarthy

A case study on quality control on telephone poles as a cost saving tool in Tanzania
1987 - IRG/WP 3418
A sample of 28 CCA treated Eucalyptus poles from a lot of 2,000 poles awaiting delivery to the field, was studied to reveal the quality of treatment. Results showed a product of very poor quality. Average figures for penetration and retention were 8.4 mm and 2.2 kg/m³; these results are 66% and 91% below the required standards, respectively. Consequences of such results are estimated to amount to losses of billion of shillings.
K K Murira

The utilisation and preservation of Eucalyptus globulus agricultural stakes from Portugal
1989 - IRG/WP 3520
This paper reviews the development and utilisation of Tanalith C treated Eucalyptus globulus stakes: particular reference is made to their use in vineyards. The treatment characteristics of commercially available stakes will be described and penetration patterns evaluated.
A Milne e Carmo, D A Lewis, A Lyman

Principles and procedure of the planeing test
1981 - IRG/WP 2162
Small end-sealed samples of pine-sapwood (1.5 x 2.5 x 5 cm³) are treated by brushing and afterwards different parts of the treated surface are removed. The remaining part of the sample is tested against either insects or fungi. If no attack occurs sufficient amounts of biocides have been penetrated at least beyond the zone which has been removed. In spite of some problems the test seems the only suitable method, to evaluate organic solvent preservatives, mainly those containing resins, for simple treating methods.
H Willeitner, M Gersonde

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