Your search resulted in 22 documents.
A summary of current information on actinomycetes and wood
1978 - IRG/WP 177
B King, R A Eaton, A A W Baecker
A method of isolating actinomycetes from decayed wood
1974 - IRG/WP 126
This paper deals with a tentative method of isolating Actinomycetes from dacayed wood....
T Haraguchi
A technique for the quantitative isolation of Actinomycetes from decayed wood
1980 - IRG/WP 1116
A technique combining comminution and high-speed homogenisation has been developed for the preparation of suspensions of micro-organisms in colonised wood. When standard inocula of Streptomyces spores were applied to wood, constant proportions of the inocula were recovered. This method was used in conjunction with selective culture media to isolate actinomycetes from wood which had been buried in ...
A A W Baecker, B King
Isolation of the dry rot fungus, Serpula lacrymans, from the forests of the Himalayan Foothills
1995 - IRG/WP 95-10129
Previous expeditions to the Himalayas (Singh 1993, 1994) have reported on the presence in this area of the dry rot fungus Serpula lacrymans. However, attempts to isolate the organism from material brought back from these expeditions were not successful. In this paper we report on the isolation of Serpula lacrymans from spores and cord material brought back from the Narkanda region of the Himalayas...
N A White, J W Palfreyman, J Singh, S Singh
The isolation of actinomycetes from wood in ground contact and the sea
1980 - IRG/WP 1110
M S Cavalcante, R A Eaton
Isolation and identification of bacteria from degraded wood - A progress report
1986 - IRG/WP 192
During attempts to isolate tunnelling bacteria in pure culture, physical separation from other bacteria was successfully achieved by the use of cellophane. Cellophane was used in liquid cultures as a substrate for tunnelling bacteria that were present in a mixture with other bacteria. By tunnelling into cellophane, TB were physically removed from contaminating bacteria which could then be removed ...
J A Drysdale, P J Ruland, J A Butcher
Isolation and identification of bacteria from CCA-treated eucalypt power transmission poles
1987 - IRG/WP 1317
Bacteria have been isolated from CCA treated Eucalyptus maculata poles which possessed characteristic bacterial decay patterns. Cultures were isolated on a number of different media which had been inoculated with a splinter of degraded wood. Primary tests indicated that most isolates belonged to the genera Pseudomonas and Bacillus. The species Pseudomonas cepacia was most frequently isolated....
G A Willoughby, A C Hayward, L E Leightley
Isolation and identification of the fungal flora in treated wood
1976 - IRG/WP 144
J F Levy
A new method for the study of microbiological decay of wood in a strictly anaerobic environment
1988 - IRG/WP 2319
Recent work has suggested that strictly anaerobic bacteria may play a role in the biodeterioration of wood, particularly in aqueous environments. However, no suitable test methods exist for the investigation of this hypothesis. An aqueous minimal medium in specifically adapted conical flasks was sterilised, gassed using a 5.5% H2; 31.8% C02; 62.7% N2 gas mixture to remove oxygen and transferred to...
G M Rogers, A A W Baecker
Isolation of soil borne bacteria and fungi from treated timber
2001 - IRG/WP 01-50174
Most research in the last few decades has focused on the development of new strategies to control biological attack and the means to quantify this. Comparatively little work has been done to examine the effect that treated timber might have on its surrounding environment. This presentation will describe a methodology that attempts to detect any changes which might occur in the soil microflora foll...
I Stephan, A Stegemann, G Heidrich
Isolation of a putative endogenous endo-ß-1,4-glucanase (cellulase) from the midgut diverticulae of the wood-boring crustacean, Limnoria quadripunctata
2003 - IRG/WP 03-10494
Over the last 75 years, many workers have conducted research into the digestive biochemistry of Limnoria. Cellulolytic enzymes have been detected in crude extracts, although whether these are of endogenous or microbial origin has been in question. To elucidate the source of these enzymes, RNA was isolated from the midgut diverticulae of Limnoria quadripunctata. Reverse transcription polymerase ...
J Dymond, M J Guille, S M Cragg
Isolation and identification of the trail pheromone of the subterranean termite Reticulitermes speratus (Kolbe) (Isoptera: Rhinotermitidae)
1990 - IRG/WP 1459
The trail pheromone was successfully isolated from whole body extract of the termite Reticulitermes speratus (Kolbe) through various chromatographic reactions. Its trail-following activity was examined by bioassays. The primary structure of the pheromone was determined as normal chain dodecatrien-1-ol by capillary GC-MS analysis. Moreover, by means of capillary GC-MS and capillary GC-FTIR analyses...
M Tokoro, M Takahashi, R Yamaoka
Isolation and identification of non-decay fungi affecting the performance of alkylammonium compounds
1993 - IRG/WP 93-10020
The isolation of DDAC tolerant fungi was carried out on lumber treated with a range of antisapstain chemicals containing DDAC as one of their active ingredients. The tolerant fungi were selected by using malt media spiked with DDAC (100 and 2500 ppm). Isolations were tentatively identified into a range of fungi commonly found associated with wood deterioration (i.e. Penicillium sp. and Trichoderma...
A K Doyle, J N R Ruddick
Isolation and identification of the fungal flora in treated wood. Revised technique
1977 - IRG/WP 159
At the 8th Annual Meeting in Wildhaus a paper was presented for discussion on the isolation of fungi from treated wood.·Since then work of this nature has been undertaken at Imperial College and as a result a revision of that document has been made and is presented here. The main alterations are: 1) To streamline the isolation procedure 2) Modification of the benomyl agar 3) The inclusion of a st...
C P Clubbe, J F Levy
Isolation of a gene from the melanin pathway of the sapstaining fungi Ophiostoma piceae using PCR
1997 - IRG/WP 97-10219
To prevent sapstaining fungi from discoloring wood, it is necessary to determine what factors affect the biosynthesis and characteristics of the pigment(s) and to identify the genes involved in the pathway. Using inhibitors and heterologous DNA probes from Alternaria alternata, we suggest that melanin, the pigment of Ophiostoma piceae, is produced by the dihydroxynaphthalene (DHN) pathway. Recentl...
R Eagen, J Kronstad, C Breuil
Evaluation of fungi isolated from the galleries of the striped ambrosia beetle Xyloterus lineatus (Ol.) (Col., Scolytidae)
1995 - IRG/WP 95-10092
Fungi from the galleries of the striped ambrosia beetle Xyloterus lineatus (Trypodendron lineatum) (Ol.) found in spruce logs were studied. The following fungi were isolated and identified: Trichoderma harzianum Rifai, the yeast Pichia anomale Hansen (Kurzman), the blue-stain fungus Ceratocystis piceae (graphium) (Münch) Bakshi and a fungus closely similar to Ceratocystis araucariae (anamorph). T...
G Babuder,F Pohleven
Isolation and characterization of hydroxyl-radical-producing glycopeptide genes from the white-rot basidiomycete Phanerochaete chrysosporium
2006 - IRG/WP 06-10588
During wood decay, the white-rot basidiomycete Phanerochaete chrysosporium secretes low-molecular-weight glycopeptides that catalyze a redox reaction between O2 and electron donors to produce hydroxyl radical. This reaction accounts for most of the hydroxyl radical produced in wood-degrading cultures of P. chrysosporium. In combination with phenol oxidases, hydroxyl radical is believed to play a r...
H Tanaka, G Yoshida, Y Baba, K Matsumura, S Itakura, A Enoki
Isolation and identification of stain fungi and mould fungi on bamboo wood in China
2009 - IRG/WP 09-10703
One hundred and seventeen isolations were isolated from 150 specimens of mildewed or stained bamboo wood and bamboo products from 9 locations in north, south and central China. The molecular (ribosomal DNA Internal Transcribed Spacer, rDNA-ITS) and morphological identification results showed these fungi could be categorized 2 phyla, 2 classes, 4 subclasses, 10 orders, 15 families, 22 genera, 56 sp...
Xingxia Ma, Mingliang Jiang, Daochun Qin
Isolation and evaluation of Lactobacillus brevis from chilli waste for potential use as a wood preservative
2011 - IRG/WP 11-10749
Lactic acid bacteria were isolated from chilli waste and evaluated for their ability to arrest wood rotting basidiomycetes. In previous work a quick screening method using 96 well plates and measuring absorbance to determine fungal growth was developed specifically to investigate the efficacy of isolated bacteria against wood decay fungi. Using this method, one bacterium (isolate C11) was identif...
D O’Callahan, T Singh, I R McDonald
Characterization of test fields
2013 - IRG/WP 13-20508
Test field characteristics and impact of test fields on wood degradation is important when testing wood protection. The current EN 252 standard has no requirement for knowledge of decay hazards, but most commonly a test field is known as a “brown”,- “white”,- or “soft rot” field. To understand which decay hazard wood preservatives are tested against, each test field should be character...
U Råberg, N Terziev, G Daniel
Isolation of the microbiota from wood decay insects and some of their enzymatic activities
2023 - IRG/WP 23-11014
Wood decay organisms have the ability to degrade wood components (cellulose, hemicellulose and lignin) by enzymatic capacities, but these enzymes can belong to the proper organism, or to the associated microbiota. The identification of the microorganisms, as well as their role in the digestion of many substrates not always is very well known.
The wood decay insects produce big damages in wood str...
M T Troya, F Llinares, J Arranz, S Izpura-Luis, S M Santos, L Robertson
Comparison of culturable fungi found on two types of modified wood cladding
2023 - IRG/WP 23-11025
Modified woods are rapidly gaining popularity in the United States and are appearing on more public buildings. Because these are biocide-free products, they can support the growth of non-wood degrading fungi on their surfaces, many of which produce dark pigments and deface the cladding. Even growth of dark pigmented fungi on modified woods yields an even grey-black color, giving the materials a we...
G Presley, J Cappellazzi, B Held, R Blanchette