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Effect of Trichoderma harzianum on induction of laccase by Trametes versicolor on ponderosa pine sapwood
1996 - IRG/WP 96-10177
Trichoderma harzianum has long been studied as a possible biocontrol agent against wood degrading fungi, and has provided varying degrees of protection in several European field trials. In laboratory trials, however, this fungus appears to be less active against white rot fungi. This characteristic was studied using a wood wafer sandwich procedure which simultaneously expose ponderosa pine sapwood...
E A Canessa, J J Morrell


An alternative method for the determination of lignocellulolytic enzymatic activities of white rot fungi
1998 - IRG/WP 98-10261
Some lignocellulolytic activities of wood decay fungi can be evaluated in an indirect way through the study of the decolorization that white rot fungi produces in culture media in the presence of dyes. In this assay, ten dyes were selected, which were added in concentrations of 0.00 1% - 0.003% (P/V) to four-day cultures of Trametes versicolor containing 1% of beech sawdust in a basal salt medium...
D Muñoz-Mingarro, F Llinares, F Rubio, M Yuste, C Rodríguez-Borrajo, J Jiménez, M T De Troya


Enzymatic study of Ceratocystis sp., blue-stain fungi on Pinus nigra
1999 - IRG/WP 99-10315
One of the main problems that the forest exploitation industry has with Pinus nigra wood is the blue-stain fungi, whose causing agent is unknown. Therefore, the objective of this work has been to study, through enzymatic tests of the isolated cultures, if these fungi infect Pinus nigra in any specific way. After the incubations, isolates of Ceratocystis were obtained. These were cultured in a sali...
M T De Troya, F Rubio, D Muñoz-Mingarro, F Llinares, C Rodríguez-Borrajo, M Yuste, M J Pozuelo, J I Fernández-Golfín


Degradation of polycyclic aromatic hydrocarbons by laccase
1998 - IRG/WP 98-10267
The degradation of polycyclic aromatic hydrocarbons (PAH) by laccase was investigated. Laccase catalyzed oxidation of phenanthrene, solubilized in the surfactant Tween 20, exhibited an absolute requirement for the mediator compound 1-hydroxybenzotriazole. When Tween 80 was used as the solubilizing agent, the oxidation rates were more than six times higher as compared to Tween 20. Using the thiobar...
S Böhmer, E Srebotnik, K Messner


Extracellular laccase production in brown-rot fungus Coniophora puteana
2001 - IRG/WP 01-10391
Coniophora puteana, one of representing brown-rot fungus, was investigated in terms of extracellular ligninolytic enzyme production. Three fungal strains of C. puteana were inoculated in liquid culture medium with sawdust of pine and oak wood. Extracellular laccase production was observed in some strains of C. puteana in the culture medium with sawdust of oak wood. The highest activity occurred at...
Kwang-Ho Lee, Seung-Gon Wi, Yoon Soo Kim


Phenol oxidase activity and one-electron oxidation activity in wood degradation by soft-rot deuteromycetes
2007 - IRG/WP 07-10615
Wood degradation, one-electron oxidation activity as assayed by ethylene generation from 2-keto-4-thiomethylbutyric acid (KTBA), and phenol oxidase activity were measured in cultures of six deuteromyce fungi, with glucose or wood as the carbon source. The four fungi that degraded Japanese beech wood had higher one-electron oxidation activities in wood-containing cultures than in glucose-containing...
H Tanaka, M Yamakawa, S Itakura, A Enoki


Effect of wood polymers degradation during heat treatment on extracellular enzymatic activities involved in beech degradation by Trametes versicolor
2008 - IRG/WP 08-40392
Effect of heat treatment on extracellular enzymes involved in wood degradation by Trametes versicolor was investigated. Heat-treated and untreated beech blocks were exposed to T. versicolor on malt agar medium and extracellular enzymatic activities investigated. A strong ABTS oxidizing activity has been detected during the first stage of colonization in both cases, while cellulase activities are m...
S Lekounougou, G Nguila Inari, M Pétrissans, S Dumarçay, J P Jacquot, E Gelhaye, P Gérardin


Degradation of polychlorinated organic biocides by the wood decaying fungi
2008 - IRG/WP 08-50253
Organochlorine biocides such as lindane, pentachlorophenol sodium salt, and polychlorinated biphenyl 153 have been widely used as wood preservatives. Due to their low degradability by soil bacteria, treated waste wood products pose a serious threat to the environment. Consequently, there is an increased need for development of new strategies for their detoxification. In this work, the bioremediati...
I Vidic, L Zupancic-Kralj, K Sepcic, F Pohleven


Fungal laccases as a tool for wood functionalization
2013 - IRG/WP 13-20533
Wood degrading fungi are the most important production organisms for laccase, a promising oxidative biocatalyst with numerous biotechnological applications in the forest products industry. Laccases are multi-copper containing oxidases (EC 1.10.3.2), which catalyse mono-electronic oxidation of phenols, polyphenols and anilines at the expense of molecular oxygen. Interest in these essentially ‘eco...
M Schubert


A novel enzymatic treatment as a new approach for wood protection
2015 - IRG/WP 15-40701
Wood durability is a worldwide concern and most treatments tend to fail with regard to their efficacy along the time. Weather conditions lead to a loss of the treatment substances into the surrounding environment and, in consequence, generate also a public health concern. Recently, the employment of oxidative enzymes, such as laccases, has been proposed as a tool for lignocellulosics modificatio...
C Fernández-Costas, S Palanti, M Ángeles Sanromán, D Moldes


Protection of bamboo against fungi by Laccase-Catalyzed Iodination
2016 - IRG/WP 16-30694
This paper investigated the effectiveness of bamboo treated by laccase-catalyzed oxidation of potassium iodide. Hence, the indoor and outdoor mold fungi resistances were carried out according to the AWPA (2007) Standard E24–06. According to the results, after 35days good resistances against the fungi were in the order of Trichoderma viride > Penicillium citrinum >Aspergillus niger. It was also o...
K P Nayebare, Shaoyong Zhang, Fangli Sun, B Goodell


Wood properties modification by an enzymatic assisted treatment with phenolic extractives
2016 - IRG/WP 16-30696
Extractives of sawdust and bark from P. pinaster and, condensed tannins (CTs) from P. radiata bark were used to treat beech and pine veneers in combination with an oxidative enzyme, in order to stably graft such extractives onto the veneers. First, the antifungal activity of P. pinaster phenolic extractives against wood decay fungi was studied. These extractives were also used to hydrophobize pi...
D Filgueira, C Fernández-Costas, D E García, D Moldes


New perspectives for wood protection: enzyme-based treatments
2016 - IRG/WP 16-40742
Wood protection research is continuously looking for more benign and effective treatments. One of the main concerns deals with the toxicity of the active compounds employed in the formulations of the preservatives, since they could become a health and environmental issue. Secondly, long-lasting treatments are preferred in order to increase the service life of the treated wood. Weathering condition...
C Fernández-Costas, S Palanti, M Á Sanromán, D Moldes