Your search resulted in 8 documents.
Molecular methods as a tool within the field of wood protection – available methods and new possibilities
2007 - IRG/WP 07-20361
The area of wood protection is in a period of change. New tools are needed to understand the mode of action, and to further improve the new wood protection systems. A set of useful tools are found among the molecular methods. This paper presents an overview of some of the tools available, and the methods are exemplified by papers within the frame of wood protection issues.
However, there is stil...
G Alfredsen, A M Hietala, C G Fossdal, H Solheim
Durability and fungal colonisation patterns in wood samples after six years in soil contact evaluated with qPCR, microscopy, TGA, chitin- and ergosterol assays
2009 - IRG/WP 09-20402
There is a need to establish new objective and sensitive methods for early detection and quantification of decay fungi in wood materials. Molecular methods have proven to be a useful tool within wood protection issues, however, this field is still poorly explored and so far relatively few have used these methods within the field of wood deterioration. Among the techniques used in the indirect quan...
A Pilgård, G Alfredsen, I Børja, C Björdal
Basidiomycete colonization of Scots pine sapwood quantified by qPCR and TGA
2011 - IRG/WP 11-10750
Scots pine (Pinus sylvestris L.) is an important softwood species in Northern Europe and is frequently used as material for various wood protection systems. In Europe, EN 113 is the standard basidiomycete laboratory durability test method, using mass loss as evaluation criteria. In this paper quantitative real-time PCR (qPCR) and thermogravimetric analysis (TGA) was used to characterize coloniza...
G Alfredsen, S Bollmus, T K Bader, K Hofstetter
Molecular investigation of Postia placenta growing in modified wood
2011 - IRG/WP 11-10756
Brown rot is the most common and destructive type of fungal decay for wood in service. These fungi depolymerize preferentially the structural carbohydrates, cellulose and hemicellulose in the cell wall leaving oxidized lignin behind. Modified wood can provide protection against a variety of wood deteriorating organisms, including decay fungi. However, there is still little known about the mode of ...
B Schmöllerl, G Alfredsen, C G Fossdal, M Westin, A Steitz
Communities of mold fungi on flooded building materials
2013 - IRG/WP 13-10799
A small building built to residential code was flooded using farmland pond water to a depth of two feet at Tuskegee University. The building was drained and left enclosed for an additional three weeks. A total of 168 material samples were removed either immediately after opening (wet) or seven months after flooding (dry). Wall materials sampled included fiberglass batt insulation, gypsum wallboard...
F Skrobot III, H Aglan, S V Diehl
Postia placenta cellulase gene expression in modified wood during incipient decay
2013 - IRG/WP 13-40626
In optimization of modified wood, it is important to understand the mode of action of the wood modification and how the fungi response to it. The aim of this study was to investigate the expression of cellulases during the first two weeks of Postia placenta exposure in acetylated, DMDHEU-treated and thermally modified as well as in untreated wood. Using real-time PCR, the gene expression patterns ...
R Ringman, A Pilgård, K Richter
Colonization of treated and untreated decking materials by bacteria, fungi and algae quantified by qPCR
2015 - IRG/WP 15-20568
The use of durable wood species for wood decking in outdoor applications, e.g. garden terrace (use class 3) is gaining more and more interest on the part of consumers and architects. To get detailed information on the practical use of deckings, a project was started in 2010. In total 71 different decking materials (0.03 m²) were installed and evaluated over 30 months. Beside other parameters, the...
E Stoyanova, B Schmöllerl, A Steitz, N Pfabigan, R Gründlinger
Different levels of acetylation lead to groupwise upregulation of non-enzymatic wood degradation genes of Rhodonia placenta during initial brown-rot decay
2020 - IRG/WP 20-10958
Rhodonia placenta, often used as a model fungus to represent brown rot fungi, uses a two-stepped degradation mechanism to degrade wood. Regarding the overcoming of wood protection systems the initial degradation phase seems to be the crucial point. A new laboratory test enables the separation of the non-enzymatic oxidative and the enzymatic degradation phases, which has previously been proven chal...
M Kölle, R Ringman, A Pilgård