IRG Documents Database and Compendium


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Effect of methylene bisthiocyanate on morphology and ultra-structure of a sapstain fungus, Ophiostoma floccosum
2003 - IRG/WP 03-10471
In vitro effects of methylene bisthiocyanate (MBT) on hyphal morphology and ultrastructure of Ophiostoma floccosum were examined using differential interference contrast, epifluorescence and transmission electron microscopy (TEM). Differential interference contrast microscopy suggested that MBT caused rapid changes to the morphology of O. floccosum resulting in excessive formation of vacuoles and...
T Singh, B Kreber, R N Wakeling, A Stewart


Controlling the sapstain fungus Ceratocystis coerulescens by metabolites obtained from Bjerkandera adusta and Talaromyces flavus
1993 - IRG/WP 93-10024
Sapstain causes severe damage to wood and wood products, posing a major economic problem for the wood industry. The purpose of this study was to determine if metabolites from Bjerkandera adusta and Talaromyces flavus would (1) decolorize stain in wood caused by Ceratocystis coerulescens and (2) prevent sapstain by Ceratocystis coerulescens. We studied the interaction of the sapstain fungus Ceratoc...
S C Croan, T L Highley


Organic nitrogen in wood: Growth substrates for a sapstain fungus
1993 - IRG/WP 93-10019
The nitrogen content of mature wood from lodgepole pine was 0.046% as determined by micro-Kjeldahl. Low molecular weight proteins between 14 and 21 kDa were identified in mature wood, branch wood and sap pressed from wood chips. Amino acids were quantified in acid-hydrolyzed wood samples and accounted for between 19 and 35% of the nitrogen in wood. The sapstaining ascomycete Ophiostoma piceae stra...
L D Abraham, C Breuil


Quantification of methylene bis thiocyanate in wood and its effect on a sapstain fungus Ophiostoma floccosum
2006 - IRG/WP 06-10591
The mobility and concentrations of MBT at different depths of wood billets (200 mm long, 40 mm in diameter) were determined using NMR spectroscopy and ICP-ASE, and then correlated the effect of wood MBT concentrations on growth of O. floccosum in a bioassay using stereomicroscopy and confocal laser scanning microscopy (CLSM). The 13C NMR spectra showed the presence or absence of MBT in wood but w...
T Singh


Biological control of the sapstain fungus Diplodia pinea (syn. Sphaeropsis sapinea) in the field
2008 - IRG/WP 08-10663
Diplodia pinea, syn. Sphaeropsis sapinea, is well known as the worldwide ocurring causal agent of the Diplodia tip-blight disease on various pine species, leading to great losses of valuable wood in the forests. Moreover, this fungus is also responsible for the fornation of sapstain of felled logs, leading to undesired disation of surface layers in the OSB manufacturing industry. In Central Europe...
C Gradinger, T Boisselet, K Messner, K Fackler


Image analysis for mould and sapstain detection on wood
2013 - IRG/WP 13-20519
Laboratory tests for mould growth on wood products are time consuming and rely on visual assessments of specimens utilizing subjective rating systems. Accelerated laboratory methods are needed that can provide rapid, quantitative assessment of mould and sapstain growth on solid and composite wood products. Image analysis of scanned spore imprints from southern pine or oriented strandboard (OSB) sp...
C A Clausen, V W Yang


Which fungi cause sapstain in Canadian softwoods?
1998 - IRG/WP 98-10285
The Canadian forest products industry suffers considerable losses in revenue due to fungal stains. There is an increased awareness that more complete knowledge about the causal organisms might help solve the problem. A first step was to initiate a thorough survey of bluestain fungi in Canada. Systematic sampling was done at seven selected sawmills in six Canadian provinces. In summer 1997 fresh lo...
A Uzunovic, Dian-Qing Yang, P Gagné, C Breuil, L Bernier, A Byrne


Screening potential preservatives against stain and mould fungi on pine timber in Zimbabwe
1995 - IRG/WP 95-30063
The search for environmentally and toxicologically safer chemicals for use in the timber preservative industry against stain and mould fungi has been intensified during the past few years. Results of field tests with two chemicals previously evaluated in the laboratory are presented. The conventional sodium pentachlorophenate was the more efficacious chemical against stain and mould fungi, providi...
A J Masuka


Improvements of monitoring the effects of soil organisms on wood in fungal cellar tests
1996 - IRG/WP 96-20093
Accelerated testing the durability of preservative treated timber in a so called "fungal cellar" or "soil-bed" to evaluate its performance in ground contact is widespread practice. In order to obtain a more accurate and reproducible estimate of preservative performance, several institutes, among them the BAM in Berlin, have routinely carried out static bending tests in addition to visual examinati...
I Stephan, S Göller, D Rudolph


The dry rot fungus Serpula lacrymans. Examples of attack and remedial treatment
1988 - IRG/WP 1347
The film deals with several aspects of dry rot attack and eradication in buildings. The detailed biology and morphological charasteristics of the fungus are portrayed. The various forms of mycelial growth, the role of the strands in the nourishment and spread of the fungus, as well as the many types of fruitbody formation are outlined. Environmental and nutritional requirements of the fungus as we...
G Buchwald, B M Hegarty, W Metzner, R Pospischil, H Siegmund, P Grabow


Improved techniques designed for evaluation of fungicides in soil for control of dry rot fungus Serpula lacrymans
1985 - IRG/WP 2238
Improved techniques provide a laboratory method for the evaluation of chemicals in soil for control of dry rot fungus Serpula lacrymans. Results with their application to three chemicals were reported. These techniques are useful to eliminate chemicals lacking the necessary toxicity and weatherbility for dry rot control when the chemicals have been applied to the soil....
M Takahashi, K Nishimoto


Japan's comments on ISO/DIS 12583-1/2
1996 - IRG/WP 96-20100
The paper describes an accelerated field test for the evaluation of timber preservative formulations against subterranean termites. The method has been adopted by the South African wood preservation industry as a screening method for the approval of wood preservatives for use under SA conditions. The method which is based upon the fungal cellar test offers a rapid means of evaluating the comparati...
P Turner, D Conradie


The accelerated field simulator (= fungal cellar)
1982 - IRG/WP 2170
G C Johnson, J D Thornton, H Greaves


The decay resistance of chemically modified aspen composites to the white rot fungus Coriolus versicolor (L.) Quelet
1998 - IRG/WP 98-40122
Chemical modification of Aspen wood (Populus tremula L.) in the form of solid wood, veneers and sawdust was undertaken by a two step procedure consisting of esterification with maleic anhydride (MA) and subsequent oligoesterification with MA and glycidyl methacrylate (GMA) or allyl glycidyl ether (AGE). Modified wood was thermoplastic and was thermally formed by hot-pressing to produce veneer or s...
M C Timar, A J Pitman, M D Mihai


Synergistic effect of boron on Streptomyces rimosus metabolites in preventing conidial germination of sapstain and mold fungi
1992 - IRG/WP 92-1565
We evaluated the synergistic effect of boron (4% BAE solution of Tim-Bor or 4% boric acid) on Streptomyces rimosus metabolites in preventing spore germination of sapstain and mold fungi using plate bioassay, Southern yellow pine and sweetgum block tests, and green pine log sections: sapstain -- Ceratocystis coerulescens, Ceratocystis minor, and Aureobasidum pullulans; mold fungi -- Aspergillus nig...
S C Croan, T L Highley


Comparison of Different Methods for Assessing the Performance of Preservatives in the BAM Fungus Cellar Test
1998 - IRG/WP 98-20149
The fungus cellar test is a common means to get reliable data on the long term performance of treated wood in soil contact. A constantly high humidity and a suitable of water holding capacity for a range of micro-organisms provide high decay rates in untreated wood and produce intensive microbial pressures on wood treated with biocides. Presently a range of biocides are under test in the BAM fungu...
I Stephan, M Grinda, D Rudolph


Chemical compounds from Eperua falcata and Eperua grandiflora heartwood and their biological activities against wood destroying fungus (Coriolus versicolor)
2005 - IRG/WP 05-30373
The chemistry analysis of the compounds present in dichloromethane and ethanolic fraction as well as bioassays enables to understand the durability differences of Eperua falcata and Eperua grandiflora. The principal distinction between these two species is the acidic subfraction of diterpenoic extract, which is antifungic in Eperua falcata when tested in in-vitro conditions. This study also enable...
N Amusant, C Moretti, B Richard, E Prost, J M Nuzillard, M-F Thévenon


The dry rot fungus and other fungi in houses. Part 2
1993 - IRG/WP 93-10001
J Bech-Andersen


Protocol for evaluation and approving new wood preservative
1985 - IRG/WP 2159
M E Hedley, J A Butcher


The dry rot fungus and other fungi in houses. Part 1
1992 - IRG/WP 92-2389
J Bech-Andersen


Commercially available anti-sapstain chemicals in New Zealand - An update
1987 - IRG/WP 3416
Six anti-sapstain chemicals or mixtures (NaPCP plus borax, Haipen 5F, Mitrol PQ375, Busan 1009, Protek S, Pinefol 50W) are available as commercial treatments in New Zealand. A further two (Hylite 20F and Isothon-35) have shown potential in field and mill trials and will be available for use. A number of other formulations are under evaluation....
J A Drysdale


Discussion of diiodomethyl p-tolyl sulfone (Amical® 48) as a fungicide for wood preservatio
1987 - IRG/WP 3425
The effectiveness of diiodomethyl-p-tolyl sulfone (Amical® 48) as a fungicide for preservation of wood is supported by a discussion of results from the literature and current research programs....
J M Stamm, K J Littel, F M H Casati, M B Friedman


Performance of Tebbacop in laboratory, fungus cellar and field tests
2000 - IRG/WP 00-30222
A novel organo-copper wood preservative ("Tebbacop") has been exposed to 12 years fungus cellar testing and 6 years above ground field testing. In the fungus cellar, Tebbacop at a retention of 0.053% Cu m/m oven dry wood out-performed CCA at a retention of 0.55% m/m oven dry wood. In above ground tests, L-joints treated to a Tebbacop retention of 0.012% Cu are performing as well as joints treated ...
M E Hedley, D R Page, B E Patterson


In search of alternative antisapstain chemicals for use in Papua New Guinea
1988 - IRG/WP 3472
The paper presents results of antisapstain field trials from three locations in Papua New Guinea as part of the Research Centre's programme to find suitable antisapstains to replace the hazardous sodium pentachlorophenate. Effectiveness of seven tested chemicals varied between indigenous pines (Araucaria cunninghamii, Araucaria husteinii) and white coloured hardwoods (Alstonia scholaris, ...
A Oteng-Amoako


Observations on the colonization of freshly-felled timber treated with prophylactic chemicals by mould and sapstain fungi
1989 - IRG/WP 1394
Field tests using freshly felled pine sapwood were set up to determine the effectiveness of a range of antisapstain compounds and to study the problems of colonization by mould and sapstain fungi. Differences were recorded both in the overall performance of the compounds and also their selectivity in controlling specific fungal types. These results were found to be useful in gaining a better under...
G R Williams, D A Lewis


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