Your search resulted in 65 documents. Displaying 25 entries per page.
Identifying the fungal community on western redcedar (Thuja plicata) wood in field tests above and in ground contact exposure: preliminary results
2019 - IRG/WP 19-10939
…14 years. We performed DNA metabarcoding using Illumina sequencing and traditional culturing techniques to characterize the fungal community from samples. Preliminary results from both culture studies and DNA metabarcoding show a predominance of ascomycetes on decks. Basidiomycetes were more frequent on fence…
A Dale, S Kus, R Stirling
Molecular identification of marine borers causing wood deterioration on marine facilities in Korea
2019 - IRG/WP 19-10955
…Chelura sp. and Teredo sp. As a result of DNA sequencing, it was confirmed that they were Grandidierella japonica and Teredo navalis. However, the homology of Chelura sp. was low, so it was difficult to identify by this result alone. As the combination of results between morphology and molecular…
Sae-Min Yoon, Min-Ji Kim, Won-Joung Hwang, Yong-Seok Choi, Dong-Won Son
Imports of African logs, do they harbor wood-decaying alien fungi and bacteria that could become a potential threat at temperate latitudes? – First results
2018 - IRG/WP 18-10904
…on imported logs by identification of fruit bodies by DNA sequencing of the internal transcribed spacer (ITS) regions ITS1 and ITS2. First results revealed the existence of at least one alien fungus on the logs. As only few fruit bodies were found on the logs we also extracted DNA from mycelium and from wood samples…
J Trautner, M Höpken, E Melcher
The copper-transporting ATPase pump and its potential role in copper-tolerance
2016 - IRG/WP 16-10859
…complex mechanism. Annotation of the Fibroporia radiculosa genes involved in copper-tolerance characterized a subset of proteins, three copper-transporting ATPase pumps, which regulate copper concentrations inside the fungal cell by exporting excess copper ions. The goal of this study was to determine the relevance…
K M Ohno, C A Clausen, F Green III, G Stanosz
Real-Time PCR Assays for the detection of the dry rot fungus Serpula lacrymans and its close relative Serpula himantioides
2016 - IRG/WP 16-10867
…the multi-copy ITS and the single-copy beta-tubulin gene (BET). Thus, the diagnostic findings can be confirmed by two independent detection parameters. It was expected that the high specificity of the TaqMan probe technology prohibits the formation of unspecific byproducts and PCR artifacts. As a result of the…
K Jacobs, N Rangno
Bacterial Degradation of Pentachlorophenol Indicated in Groundwater Undergoing Long Term In-Situ Remediation
2016 - IRG/WP 16-50318
…using molecular techniques. Bacteria were identified by sequencing the 16s rDNA fragment from DNA extracted from groundwater cultures and comparing those sequences to a database using a basic local alignment search tool, BLAST. The PCP-degraders Burkholderia cepacia and Flavobacterium (Sphingobium)…
C E Stokes, H Borazjani, L Prewitt
Estimation of wood degradation determined by visual inspection, mechanical testing and DNA characterisation – report after 3 years of exposure
2016 - IRG/WP 16-30700
The influence of two separate treatments, thermal modification and alkaline copper quaternary (ACQ, Silvanolin©) treatment, on fungal decay, visual disfigurement, deterioration in mechanical properties and fungal community structure was assessed on Norway spr…
N Thaler, T Martinović, M Bajc, D Finžgar, H Kraigher, A Sinha, J J Morrell, M Humar
The role of hydrophobins in surface growth by the Blue Stain fungi Aureobasidium pullulans
2015 - IRG/WP 15-10837
…exterior wood coatings (e.g. wood paints). At least two gene sequences encoding hydrophobins in Aureobasidium pullulans were identified, both to be successively disrupted by integrating a recycled selectable marker into the open reading frames of the genes, creating a double knock-out mutant strain, using…
J Stenbæk, L Riber, J Blæsbjerg Nielsen, C Møller Hansen, B Jensen
Emerging function for a glutathione transferase in response to wood extractives toxicity
2014 - IRG/WP 14-10824
…a transcriptomic analysis of Phanerochaete chrysosporium genes was performed in presence of oak acetonic extractives. The most up-regulated genes relate to nutrition, nucleic acid modification, gene regulation, signalling and stress responses. Focusing on the induced antioxidant and detoxification systems, a…
A Thuillier, K Chibani, A Deroy, S Dumarçay, P Gérardin, A Kohler, J-P Jacquot, E Gelhaye, M Morel-Rouhier
Identification of soft-rot fungi existed in the samples from the galley excavated at Yenikapi
2014 - IRG/WP 14-10833
…(Köse and Taylor 2013). In this study, therefore, genetical approaches using rDNA ITS region was conducted to identify fungal species existed in the samples. Six ascomycete strains were detected, and one of them was Penicillium paneum, which is known as a typical soft-rot fungus of wood. We are identifying…
T Wada, C Köse, K Igarashi
Communities of mold fungi in moisture damaged building materials
2014 - IRG/WP 14-20542
…were studied by fungal ITS region targeted PCR-DGGE and sequencing. Fungal communities differed between building materials and humidity conditions. In RH 90% the majority of the sequences obtained belonged to genus Aspergillus. As expected, in RH 98% the fungal community was more diverse containing e.g. genera…
E Sohlberg, H Viitanen
Communities of mold fungi on flooded building materials
2013 - IRG/WP 13-10799
…siding, and house wrap and were analyzed by cloning and sequencing to identify the mold species present both above and below the water line. The vinyl siding and house wrap had the lowest mold growth while the batt insulation had very high quantities of mold, followed by the paper siding of the gypsum. The common…
F Skrobot III, H Aglan, S V Diehl
High-throughput sequencing highlighted contrasted pioneer fungal communities associated to coniferous and deciduous wood preservation assays
2013 - IRG/WP 13-10800
…the pioneer steps of wood degradation. The use of Next-generation DNA sequencing methods, which produce massive volumes of data, provided new perspectives in fungal molecular ecology. In this paper we used high throughput sequencing to identify factors influencing fungal colonisation during the early stages of…
Y Mathieu, A Dassé, I Le Bayon, M Kutnik, L Harvengt, E Gelhaye, M Buée
A case study of investigating fungi that affect traditional Japanese shake roof with/without copper plates
2013 - IRG/WP 13-10810
…agar (MA) medium and a non-cultural method, the next generation sequencing method. Fungal strains were isolated on MA plates from ethanol-sterilized wooden pieces and non-sterilized pieces respectively, to see fungal species rich on the surface and inside the wooden pieces. Surface sterilization resulted in a…
T Wada, Y Fujiwara, Y Fujii, R Kigawa
Characterization of test fields
2013 - IRG/WP 13-20508
…different media and identified the isolated fungi by DNA sequencing. DNA profiles were established for Scots pine mini- stakes as well as microscopy characterization of stake cross sections for decay type and degree of attack. MOE and mass loss was measured for the mini-stakes at one test field. For all fields, the…
U Råberg, N Terziev, G Daniel
Virulence of two Laboratory Test Strains and one Natural Isolate of Rhodonia (Oligoporus) placenta against Thermally Modified Pine and Beech Wood
2013 - IRG/WP 13-20524
…and molecular analysis, the last one based on sequencing the internal transcribed spacer (ITS) of the nuclear ribosomal DNA (nrDNA). As expected, the durability of beech and pine sapwood was considerably increased by thermal modification. Distinct differences were found between the virulence of the R.…
K Plaschkies, W Scheiding, K Jacobs, N Rangno
What molecular biology can tell us about the biodegradation of lignocellulose: the utilization of molecular techniques for the detection, identification and enhanced understanding of wood degrading organisms
2013 - IRG/WP 13-20528
…will be followed by a discussion of the power of sequencing and other procedures for better understanding: the mechanisms involved in the biological degradation of wood, the metabolic basis of preservative function and ultimately the evolutionary history and ecological function of some of these unique…
J Jellison, B Goodell, G Alfredsen, D Eastwood, G Daniel, S M Cragg, J K Grace
Postia placenta cellulase gene expression in modified wood during incipient decay
2013 - IRG/WP 13-40626
…as well as in untreated wood. Using real-time PCR, the gene expression patterns of the P. placenta endoglucanase Ppl103675 and β-glucosidase Ppl112501 during 56 days of decay was analyzed. Preliminary data indicate that both genes are expressed at higher values in untreated wood at 56 days of exposure (56% mass…
R Ringman, A Pilgård, K Richter
Connecting fungal genomic variations to wood chemical composition: A way to understand the adaptation of wood decaying fungi
2012 - IRG/WP 12-10776
The development of new sequencing methods allows now exploring the detoxication systems of wood-decaying fungi. These systems, called xenome, are mainly composed of oxidative enzymes (phase I) and of conjugation enzymes (phase II). These enzymes belong to superfamilies, which are…
E Gelhaye, Y Mathieu, E Meux, A Thuillier, M Mélanie
Variation in two Postia placenta strains, MAD-698-R and FPRL 280 – mass loss, DNA content and gene
2012 - IRG/WP 12-10781
…was investigated: mass loss, fungal gDNA content and gene expression. A significant difference was found in mass loss after eight weeks between the P. placenta strains MAD-698-R and FPRL 280. MAD-698-R gave higher mass loss than FPRL 280. However, MAD-698-R seems to have a slightly slower growth rate than FPRL…
N Thaler, G Alfredsen, C G Fossdal
Genetic diversity and morphometric study of thirteen populations of Microcerotermes diversus (Silvestri)(Isoptera: Termitidae) in southern Iran
2012 - IRG/WP 12-10782
…to cellulosic products in buildings. To investigate the genetic diversity of this pest, 13 populations from different areas of Khuzestan province as well as two samples from Khârg Island and Bandar Abbas were collected. After DNA extraction, a fragment with 486 bp length from the mithochondrial cytochrome oxidase…
E A Firouzabadi, B Habibpour, H Galehdari, P Shishehbor
Biodegration of treated wood waste by native fungal communities of tropical soil in French Guiana
2012 - IRG/WP 12-50285
Woods have been protected with fungicides for a long time, and the effects of these fungicides on soil after being leached into the ground have turned out to be a true environmental issue. It is in this perspective that we are proposing to study fungal communi…
A Zaremski, L Gastonguay, C Zaremski, F Chaffannel, J Beauchêne, G LeFloch
Gene expression analysis of a copper-tolerant brown rot fungus on MCQ-treated wood
2011 - IRG/WP 11-10748
…species, Antrodia radiculosa, to examine expression of genes on MCQ-treated wood. Our hypothesis was genes that decreased copper bioavailability would be up-regulated early, when wood showed no strength loss, while genes that degraded the structural polysaccharides would be up-regulated late, when wood exhibited…
J D Tang, A Perkins, S V Diehl
The effects of acetylation level on the growth of Postia placenta
2011 - IRG/WP 11-10751
…exposed to different wood protection systems the study of gene expression can give us some answers. When the DNA sequences are known, primers can be designed to detect transcripts of genes with gene products related to basic cellular processes and hyphal growth. The characteristic gene products induced in different…
A Pilgård, G Alfredsen, C G Fossdal, C J Long II
Molecular investigation of Postia placenta growing in modified wood
2011 - IRG/WP 11-10756
…the decay resistance. The biochemical mechanisms and gene products induced in brown rot during growth in modified wood are poorly understood. In this paper the data collected from mass loss studies and qPCR and qRT-PCR were used for profiling growth dynamics and gene expression of the brown rot fungus Postia…
B Schmöllerl, G Alfredsen, C G Fossdal, M Westin, A Steitz