IRG Documents Database and Compendium



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Activity of Two Strobilurin Fungicides Against Three Species of Decay Fungi in Agar Plate Tests
2017 - IRG/WP 17-30704
…activity. All treatments were incorporated in an agar-based media and evaluated for their effects on mycelial growth rate. Based on minimum inhibitory concentration values found for F. radiculosa (a copper-tolerant fungus), Insignia was 5.5x more toxic than Heritage. SHAM at 100 ppm, increased toxicity 9x…
J D Tang, T Ciaramitaro, M Tomaso-Peterson, S V Diehl


Standard Test Methods for Wood Preservatives by Laboratory Agar-Block Test
2006 - IRG/WP 06-20350
…tested for their ability to control decay fungi in agar-block tests. In an agar-block test, blocks of wood containing a pre-determined retention level of chemical are exposed to wood-destroying species of both brown-rot and white-rot fungi. The blocks along with the fungi inoculums are placed within a sealed…
P K Sarker, M A Rahman, M R Bulbul, T Das, G N M Ilias


The effect of malt and agar trademarks on growth, decay and stilbene resistance of fungi
2000 - IRG/WP 00-20202
Effects of malt and agar trademarks used in growth media on growth of fungi in cultures, amount of decay they cause in birch samples and their resistance to stilbenes were tested. It was tested also had the conditions where fungus cultures were stored before inoculation of test…
L Syrjälä, L Paajanen, A Pappinen


Protection of wood blocks treated with Trichoderma isolates selected on the basis of preliminary agar screening studies
1996 - IRG/WP 96-10154
Previously reported results of agar interaction screening studies for biocontrol agents of wood decay basidiomycetes showed two Trichoderma viride isolates, killed 16 of 19 target fungi (Tucker and Bruce, 1995). Testing of these isolates in wood was required to assess their performance at…
E J B Tucker, A Bruce, H J Staines


Fungitoxic effect of the quaternary ammonium compounds preservatives against Basidiomycetes by using agar-plate and agar-block methods
1996 - IRG/WP 96-30118
…to the wood destroying fungi have been presented. The agar-plate (screening) and agar-block method were applied. A wide range of the fungitoxic activity of all the three formulations and a very small leachability were proved.
J Wazny, P Rudniewski


Screening wood preservatives: Comparison of the soil block, agar block and agar plate tests
1993 - IRG/WP 93-20001
…In this paper the relative merits of the soil block, agar block and agar plate tests are compared. Eight commercially available biocides encompassing inorganic and organic systems were tested against four basidiomycete decay fungi. Each biocide was ranked according to its performance in the three tests. The…
K J Archer, D D Nicholas, T Schultz


Development of accelerated decay test for CLT using a fungus grown on agar medium
2019 - IRG/WP 19-20664
…mm with fungus (Fomitopsis palustris) grown in agar medium in plastic container for 3 and 6 months. As the results, the mass loss of the samples attached with two containers (samples sandwiched between two containers) was higher than of with one container. The mass loss of the samples exposed for 3 months…
T Miyauchi, K Kambara, W Ohmura, T Mori, H Matsunaga, N Hattori


Comparison between agar-block and soil-block methods for wood-destroying Basidiomycetes
1992 - IRG/WP 92-2401
…of the fungi in this method was more homogeneous than in agar-block. However the soil-block method presents some difficulties not found in the agar-block method. As related to the performance of Picnoporus sanguineus strains, the greater loss of mass occured when it was isolated from carpophore, no matter what…
M B B Monteiro, S Brazolin, G Catanozi


Basidiospore germination threshold against borate in-vitro may vary between liquid and agar media
1992 - IRG/WP 92-2405
…to prevent basidiospore germination on malt extract agar. Spore germination sensitivity was lower (50%) for only one of the white rot fungi. However, when spore germination thresholds were tested in malt extract-borate solutions in well slides (no agar), much lower thresholds were noted for 3 of 4 fungi.…
E L Schmidt, Ya-Lih Lin


Agar block durability test of plywood made of neem (Azadiracta indica) leaves treated veneer using two white rot fungi
2017 - IRG/WP 17-40782
This study has been undertaken to investigate the durability of plywood made from non-durable wood (simul, Bombax ceiba) veneer treated by neem (Azadiracta indica, A. Juss.) leaves. The veneers were treated with cold and hot water solution of neem leaves of di…
K Akhter, M A Hashem, S Akhter


Interaction between Aspergillus niger fungus and other filamentous fungi on salt-agar medium and their growth on natural wood and wood treated with fungicide
2015 - IRG/WP 15-20569
…fungi, the growth rate of Aspergillus niger fungus on salt-agar medium in comparison to other filamentous fungi and to compare its growth on Scots pine wood both natural and chemically preserved with model wood preservative. It was observed that the growth rate of Aspergillus niger (An) fungus in one-species test has…
A Fojutowski, A Kropacz, A Koziróg


Growth of selected wood decay fungi on various agar-supplemented media
2003 - IRG/WP 03-10456
…of wood decay fungi (brown and white rots) on various agar-supplemented media have been determined and compared. The agar media investigated were Malt extract agar (MEA), Potato dextrose agar (PDA), YMPG agar (yeast extract, malt extract, bacto-peptone, glucose, asparagine and thiamine), YMPG agar (without amino…
S A Amartey, M Humar, F Pohleven


Screening of bacteria, yeasts and Trichoderma isolates for antagonism toward stain and mould fungi on agar media and wood
1999 - IRG/WP 99-20159
…mould and wood stain fungi was undertaken on 8 types of agar-based media by application of several bacteria to each plate. The rapid screening method was subsequently verified by testing antagonism of the most promising bacteria on Scots pine sapwood. Most of the 64 bacterial showed antagonistic effects on certain…
C Payne, A Bruce


Utilisation of carbohydrates by stain fungi in agar culture
1998 - IRG/WP 98-10248
…and Aureobasidium pullulans (de Bary) Arnaud were grown on agar supplemented with a variety of carbon sources, and mycelial growth rates were measured to determine which carbohydrates can be used by these fungi.
J Snow, P Vinden, S M Read


The proposal for optimalization of the agar-block method for wood preservatives fungitoxic evaluation
1995 - IRG/WP 95-20065
…scientists results the proposal for optimalization of agar-block method was presented by: - selection of test fungal species and strains and central distribution of their pure cultures, - change of the treatment and control samples exposure procedure, - application of mathematical estimation of toxic value…
J Wazny


Preliminary agar screening studies as the first stage in the development of a biological control system against basidiomycetes in ground contact situations
1995 - IRG/WP 95-10111
…on three types of media. Media used were 3% malt extract agar (MEA); a low nutrient synthetic media with a carbon: nitrogen ratio typical of that found in softwoods (LNM); and a similar synthetic media where the amino acid profile represented the major constituents shown by analysis to be present in Scots pine…
E J B Tucker, A Bruce


Comparison of the agar-block and soil-block methods used for evaluation of fungitoxic value of QAC and CCA wood preservatives
1994 - IRG/WP 94-20039
The modyfied agar-block and soil-block methods were used for comparing the fungitoxic value of QAC and CCA type preservatives against Coniophora puteana and Coniophora olivacea The mass loss and moisture contents of wood were analysed.
J Wazny, L J Cookson


Effect of sterilization method on germination of spores of wood decay fungi observed by contact agar block method
1978 - IRG/WP 2117
…response of decay fungi. Germination was assessed on agar discs fused by aqueous diffusion path to 1 cm³ samples of aspen and white spruce sapwood.
E L Schmidt, D W French


Cellulose nanomaterials in growth media for wood decay fungi
2024 - IRG/WP 24-11035
…(CNFs) were investigated as a partial substitution for agar in growth media for wood decay fungi. Radial growth measurements of eight basidiomycete fungi were taken on solid growth media with and without CNFs. Additionally, fungal strain virulence was evaluated using the European CSN Standard EN 113-2 wood decay…
K M Ohno, R A Arango, R Sabo, C M Clemons, G T Kirker, A B Bishell


Cellulose nanomaterials in growth media for wood decay fungi
2024 - IRG/WP 24-11035
…(CNFs) were investigated as a partial substitution for agar in growth media for wood decay fungi. Radial growth measurements of eight basidiomycete fungi were taken on solid growth media with and without CNFs. Additionally, fungal strain virulence was evaluated using the European CSN Standard EN 113-2 wood decay…
K M Ohno, R A Arango, R Sabo, C M Clemons, G T Kirker, A B Bishell


In Vitro Growth of the Basidiomycetes Physisporinus vitreus strains in various conditions in comparison with some white rot fungi
2022 - IRG/WP 22-10987
…of P. vitreus by chemotropic test, choice test, and agar plate toxicity tests and determination of the effect of various ions on oxalic acid secretion by these white rot fungi. In the project, it is also aimed to run fungal decay tests to determine nano and soluble-Cu tolerance of the test fungi and Cu…
E E Soytürk, S N Kartal


Exploring the use of X-ray micro CT as a tool for the monitoring of moisture production and mass loss during lab-based fungal degradation testing
2019 - IRG/WP 19-20654
…durability data is available for solid wood, the standard tests, mainly measuring the mass loss caused by decay fungi after sixteen weeks, do not offer much information on what is going on inside the wood during degradation. Notwithstanding that standard tests are excellent for testing the durability of a certain…
L De Ligne, J Van den Bulcke, A De Muynck, J M Baetens, B De Baets, L Van Hoorebeke, J Van Acker


The Impact of Cryopreservation Techniques and of Water Quality on the Wood Degrading Ability of Fungal Strains: Recommendations for Maintenance and Culturing Procedures
2018 - IRG/WP 18-20636
…of water used for the preparation of the culturing malt agar medium on the mycelium growth and on the virulence of fungal strains was assessed. The vitality of all fungi was successfully preserved with cryopreservation at -80 °C during six months in sterile water with 25% or 50% of glycerol used as…
A Stum, M Montibus, I le Bayon, M Kutnik


Laboratory test to determine the effect on durability of combining biobased building materials with timber in construction
2017 - IRG/WP 17-20604
…wool or straw. Traditional laboratory based wood decay tests do not take into account this combination of biobased materials and it may be possible that non wood materials such as plant or animal fibre insulation could influence the durability of adjacent wood elements by synergistic mechanisms. One method may…
S F Curling, G A Ormondroyd


Decay resistance variability of European wood species thermally modified by industrial process
2017 - IRG/WP 17-40807
…wood species: spruce, ash, beech and poplar. All of the tests concerning the decay resistance were carried out in the laboratory using untreated beech and pine woods as reference materials. An agar block test was used to determine the resistance of thermally modified woods, leached beforehand according to EN 84…
K Candelier, L Pignolet, S Lotte, A Guyot, E Cuny, B Bousseau, M-F Thévenon