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Patterns of bacterial attack in preservative treated eucalypt power transmission poles
1984 - IRG/WP 1223
Patterns of bacterial decay have been examined using light and scanning electron microscopy. Two morphologically distinct patterns have been recognised in preservative treated eucalypt power transmission poles. Bacterial Decay I (BDI) involves the removal of the secondary wall layers initiating with erosion of the S3 layer from the cell lumen. As decay progresses the S2 layer and S1 layer are degr...
G A Willoughby, L E Leightley


Evaluation of an alkyl ammonium compound as a fungicide to control sapstain and mould during diffusion storage
1984 - IRG/WP 3282
An alkyl ammonium compound ('Akzo' ES 255) was evaluated for its effectiveness against mould and sapstain during diffusion storage of boron-treated rubber wood. Though ES 255 at 1.0% concentration was effective against mould (71%) and sapstain (89%) it is less satisfactory compared to 0.5% sodium pentachlorophenoxide against mould (92%) and sapstain (98%)....
R Gnanaharan


Ultrastructural aspects of bacterial attacks on an archaeological wood
1993 - IRG/WP 93-10007
Transmission electron microscopy of wood from a Chinese ship submerged in the mud for over 900 years showed bacteria to be the main factor for its deterioration. The micromorphology of degraded wood cell walls was similar to that observed during the attacks of wood by erosion bacteria. Other bacterial forms, previously considered lo be scavenging bacteria, were also abundant in degraded areas of t...
Yoon Soo Kim, A P Singh


Examining environmental conditions and the biodeterioration of historic waterlogged wood: the Kolding Cog
2002 - IRG/WP 02-10441
Survival of waterlogged wood from thousands and in rare cases millions of years presents scientists with a unique opportunity to examine wood specimens which, due to select properties of the wood itself and/or the depositional environment, have not been completely degraded. This paper discusses the biodeterioration of a submerged shipwreck buried in Kolding Fjord, Denmark for the past 1000 years....
B A Jordan, D J Gregory, E L Schmidt


Sapwood staining fungi
1974 - IRG/WP 125
The compilation is based on the available literature and on research results from the Royal College of Forestry, Stockholm but I am aware that there are great gaps in it and so it must be looked upon as a basis for collecting more information about these fungi. The review in the presented form needs an explanation. Preparing this review, the presented schedule was set up to facilitate summarisatio...
A Käärik


The yeast Pichia sp. As a short-term biological control agent to fungal spoilage of sawn softwood timber
2000 - IRG/WP 00-10362
Previous work has found isolates of the yeast Pichia to be a successful biological control agent toward moulding of fruits. An isolate was tested for the ability to protect sapwood of Pinus sylvestris timber against visual degrade by surface growth of moulds and staining fungi. Successful protection of autoclaved wood sprayed with a mixture of common wood moulding fungi was achieved when the yeast...
C Payne, H J Staines, A Bruce


Evaluation of New Zealand staining fungi for degradation of radiata pine
1999 - IRG/WP 99-10310
This investigation sought to determine the potential of selected NZ staining fungi to induce weight loss and changes in toughness (impact bending strength) and composition in unseasoned radiata pine sapwood. Furthermore, assays were performed to eveluate enzyme production by staining fungi. Sets of side-matched specimens were inoculated with Ophiostoma floccosum, Sphaeropsis sapinea and O. plurian...
A Schirp, R L Farrell, B Kreber


A potential anti-sapstain chemical for sawmills
1984 - IRG/WP 3300
Several biocidal formulations were tested under laboratory conditions in order to discover an alternative antisapstain chemical to chlorophenoxides. The formulations tested were experimental water solutions containing a quaternary ammonium chloride, a commercial fungicide and/or a sodium salt of some carboxylic acids. The effect of sodium carbonate and borax decahydrate in mixture with a quaternar...
I A Linderborg


Susceptibility of harvested softwoods to infection by sap-staining fungi
2002 - IRG/WP 02-10435
In the UK a field study programme was carried out to compare the susceptibility of softwood timber from five different commercially important tree species to infection by sap-stain fungi. Logs cut from freshly felled Scots pine (Pinus sylvestris), Lodgepole pine (P. contorta var. latifolia), Sitka spruce (Picea sitchensis), Norway spruce (P. abies) and Japanese larch (Larix leptolepis) were expo...
E J Young, R A Eaton, J F Webber


Some experiences with attack of microorganisms on wooden constructions supporting foundations of houses and bridges
1997 - IRG/WP 97-10232
Reconstructions of bridges and public buildings or damage of houses during the construction of subway lines in Berlin have led to a number of inspections of wooden foundations, mostly pine or spruce piles, representing service lives of between ca. 70 and 140 years. In all cases bacterial attack was found both in wood submerged in ground water and in surface water. The extent of deterioration diffe...
M Grinda


Enzyme systems of bacterial isolates from ponded logs - Potentials of pectin and/or starch degradation
2000 - IRG/WP 00-10378
This paper deals with the degradation potentials of wood constituents by the bacterial isolates from ponded logs. The potentials to degrade pectin as a constituent of pit-tori as well as starch existing in ray parenchyma cells in the areas of sap- and transition wood with the isolates were examined. The pectinase activity was investigated by means of the degradation degree of a carrot strip used a...
S Doi, S Ohta


Standardisation of sapstain tests - A challenge
1992 - IRG/WP 92-2403
In the last decade many new anti-sapstain products have been tested world-wide under laboratory and field conditions. Several extensive test programmes have been executed with different non-standardized test methods and procedures, with the result that the biological findings cannot be compared with each other. In this paper, gathered recommendations will be given in order to standardize test meth...
G Rustenburg, C J Klaver


An evaluation of chemical treatments for the protection of radiata pine logs from fungal degrade
1986 - IRG/WP 3377
Busan 30, Busan 1009, Mitrol PQ375. and sodium pentachlorophenoxide (NaPCP) plus have been evaluated as antisapstain treatments for the long-term protection of debarked radiata pine logs. After 3 months' winter-spring storage, treated debarked logs had less surface and internal sapstain than 'control' logs. After 6 months' storage all logs had internal sapstain ...
J A Drysdale, M E Hedley, J A Butcher


Using DNA probes to characterize the metabolic pathway of pigment production in several wood-staining fungi
1996 - IRG/WP 96-10146
During shipment and storage, lumber is susceptible to sapstain, a wood discoloration caused by fungi. Currently kiln drying and chemical applications are used to control sapstain. However, the chemicals used to protect wood have a broad range of action, and so can affect other organisms. In addition, in Canada most of these chemicals are under temporary registration. Thus there is a need to develo...
R Eagen, S Riecken, J Kronstad, C Breuil


Wood extractives as carbon sources for staining fungi in the sapwood of lodgepole pine and trembling aspen
1995 - IRG/WP 95-10098
Wood discoloration by sapstaining fungi reduces lumber value. In Canada, the most commonly recorded sapstaining fungi belong to the genera Ophiostoma. This work evaluated the effect that Ophiostoma piceae, Ophiostoma ainoae, and Ophiostoma piliferum used the lipids present in the sapwood of lodgepole pine (Pinus contorta Dougl.) and trembling aspen (Populus tremuloides Michx.). Wood lipids, common...
Yong Gao, C Breuil


Screening results of fungicides for sapstain control on Pinus radiata
1983 - IRG/WP 3236
Thirty-two compounds were tested to determine their ability to contain the growth of stain, mould and rot organisms on fresh Pinus radiata D Don. A screening technique was employed using 35 to 50 mm diameter biscuits of Pinus radiata stemwood 10 mm thick. No compound was found to be cost effective when compared against the standard treatments of NaPCP (0.5% a.i.) plus borax (1.5%) and Captafol (0....
P J Hayward, J Duff, W Rae


Laboratory evaluation of potential antisapstain treatments for Pinus radiata
1983 - IRG/WP 3237
Twelve formulations were tested in the laboratory using a 'mini' board test for effectiveness on Pinus radiata (D. Don) against sapstain, mould and decay fungi. Three formulations, sodium pentachlorophenoxide (NaPCP) plus borax, Haipen 50 WP, and Mitrol 375, are used commercially in New Zealand. Busan 1009 (0.5% product), IWD Product A (0.2% ai), IWD Product B (0.3% ai), Fennotox...
J A Drysdale


The effect of primary blue-staining fungi on the strength properties of Scots pine sapwood
2004 - IRG/WP 04-10515
The fungi action may have influence on wood properties especially Basidiomycotina fungi causing wood decay. The investigation of influence of fungi causing mainly wood discoloration and disfigurement on wood properties is still in development process. There were determined the differences between the bending strength, the compression along the grains and impact strength of control and blue stained...
A Fojutowski


Degradation features of waterlogged archaeological compression wood
1998 - IRG/WP 98-10258
The degradation characteristics of waterlogged archaeological compression wood excavated in South Korea were examined by transmission electron microscopy (TEM) and confocal laser scanning microscopy (CLSM). Degradation of Pinus compression wood occurred mainly in the inner part of S2 layer. In contrast, the outer part of S2 layer remained relatively intact. CLSM and TEM showed the erosion type of ...
Yoon Soo Kim, A P Singh


Effect of volatiles from bacteria and yeast on the growth and pigmentation of sap-stain fungi
2000 - IRG/WP 00-10331
Sapstain fungi cause deterioration of wood due to colonisation by pigmented hyphae but without producing significant strength losses. This is due to the production of melanin in the fungal cell walls of the staining fungi. Any biological control strategy targeted against this type of deterioration would therefore be considered successful if it inhibited either fungal growth or pigment production. ...
A Bruce, R E Wheatley, S Verrall


Mixtures of fungicides screened for the control of sapstain on Pinus radiata
1984 - IRG/WP 3307
Fourty nine mixtures of fungicides were evaluated in a rapid laboratory screen for the control of stain, mould and decay fungi on Pinus radiata (D.Don). The most effective mixture was thiophanate methyl plus chlorothalonil closely followed by the mixtures of thiophanate methyl plus benzisothiazolone and thiophanate methyl plus dithio-bis (benzmethylamide). Benzalkonium chloride, thiram and ziram, ...
P J Hayward, W Rae, J Duff


Secretion of ligninolytic enzymes by hyphal autolysis of the white rot fungus Phanerochaete chrysosporium
1991 - IRG/WP 1480
The secretion of ligninases by Phanerochaete chrysosporium was investigated with polyclonal antibodies, followed by immmunogold-silver staining and light microscopy. After growing fungal mycelium on nitrocellulose, extracellular ligninases were detected around old, plasmaless hyphae, but not at arthrospores, chlamydospores, or blastoconidia. Labeling of fungal hyphae on coverslip cultures was obse...
R Lackner, E Srebotnik, K Messner


Blue stain in timber in service. Results of further IRG collaborative tests to compare different artificial weathering systems
1981 - IRG/WP 2146
The previous work described in Document No IRG/WP/286 examined 3 preservatives (1% phenyl mercury succinate - PMS, 5% pentachlorophenol - PCP and 1.5% Preventol A4 - PA4) subjected to 5 different artificial weathering systems (BAM Xenotest, Cuprinol Marr, EMPA Xenotest, Gori Atlas and PRL Marr) and after natural weathering at 5 sites (BAM, Cuprinol, EMPA, Gori and PRL). Results showed good correla...
A F Bravery, D J Dickinson


Assessment of treated wood leachates genotoxicity with a bacterial test
1997 - IRG/WP 97-50089
Genotoxicity is known as the damage caused by environmental stressors (biological, chemical and physical) on the genetic material of an organism. This toxicological effect can be assessed by a lot of biological assays and especially by bacterial tests. These tests are frequently performed on environmental samples or on pures substances and are in that case, strongly correlated to the carcinogenic ...
P Marchal, C Martin


Integrated protection of freshly sawn lumber using Bacillus subtilis and selected fungicide
1997 - IRG/WP 97-10235
Bioprotection against stain fungi has tremendous potential for reducing discoloration of freshly sawn wood while decreasing chemical consumption. Unfortunately, most bioprotectants appear to be unable to consistently perform under the array of conditions to which freshly sawn wood is exposed. While research is underway to understand the nature of the inconsistent performance, a more pragmatic appr...
M E Mankowski, M Anderson, J J Morrell


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