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Basidiosporogenesis by the white-rot basidiomycetes in vitr
1994 - IRG/WP 94-10081
Basidiospores of all forest-inhabiting basidiomycetes are a primary source of infection causing wood decay. However, most studies evaluating wood preservatives have used mycelia or basidiospores obtained from wild mushrooms. The objective of this study was to demonstrate in vitro methods that promote carpogenesis and basidiosporogenesis by the white-rot basidiomycetes, Schizophyllum commune and Trametes versicolor. After preincubation in the dark at 27°C for three to fifteen days, basidiospores were produced in four to sixteen weeks in basidiomes exposed to light at 12°C. Adequate light exposure, aeration, and low temperature treatment after preincubation are essential for fruiting body of these white-rotting basidiomycetes. Carpogenesis and basidiosporogenesis of Schizophyllum commune is controlled by nitrogen and carbon limitation. However, fruiting body formation in Trametes versicolor was induced by nitrogen limitation. Walset cellulose was found to be the best carboun source for carpogenesis and subsequent basidiosporogenesis. These results demonstrate the feasibility of using various potential inhibitors on basidiospore germination rather than relying on mycelial growth.
S C Croan


Basidiosporogenesis by brown-rot basidiomycetes in vitro
1995 - IRG/WP 95-10126
Basidiospores of wood-rotting basidiomycetes are a primary sourse of infection causing wood biodeterioration, especially in wood above ground. Most studies evaluating wood preservatives have used mycelia or basidiospores obtained from wild mushrooms. The objective of this study was to demonstrate in vitro methods that promote carpogenesis and basidiosporogenesis by the brown-rot fungi Antrodia carbonisa, Neolentinus lepideus, and Postia placenta. After preincubation in the dark at 27°C for 3 to 11 days, basidiospores were easily prodused in 1 to 4 months by basidiomata exposed to light at 12°C. Adequate light exposure, aeration, and low temperature treatment after preincubation are essential for fruiting body formation of these brown-rot basidiomycetes. The morphology of the basidiomata differed according to the basidiomycetes and the medium used. These results demonstrate that an enormous quantity of basidiosporee can be easily and continuously prodused in 2 to 4 months in vitro.
S C Croan


Fruitbody formation and basidiosporogenesis by the white rot fungus Pycnoporus cinnabarinus
1988 - IRG/WP 1348
Conditions for fruitbody formation by the white rot fungus Pycnoporus cinnabarinus in vitro were outlined. A marked difference in substrate requirements in comparison to previously tested brown rot fungi was revealed. Agar media containing Walseth cellulose and NH4 tartrate at 1 g/l permit profound production of basidiospores particularly at 15°C but only under light treatment. Higher NH4 tartrate concentrations and glucose addition slowed down or inhibited the fruitbody formation. Axenic basidiospores, to be used in tests for inhibitors contributing to natural durability, as well as in tests for fungicides could thus be produced.
J Bjurman