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Étude in vitro de la colonisation et de la dégradation structural du bois de hêtre par Coriolus versicolor (L.) Quélet
1976 - IRG/WP 158
L'attaque du bois de Hêtre par Coriolus versicolor (L.) Quélet, agent de pourriture fibreuse blanche est étudiée après des périodes d'exposition à ce champignon variant de 15 jours à trois mois. L'observation des premiers stades, c'est à dire de la colonisation du bois par les filaments de champignon est faite en microscopie optique, puis au fur et à m...
D Dirol


Effect of wood moisture on ability of Sphaeropsis sapinea to colonise Pinus radiata
1999 - IRG/WP 99-10311
The factors influencing colonisation of unseasoned radiata pine logs by sapstaining fungi which can result in detrimental wood discolouration, are being explored. The objective of the current study was to determine the effect of wood moisture content on fungal growth and wood colouration (melanisation). Five replicate trials were set up using increment cores (10mm diameter) which were taken from ...
B Kreber, D R Eden, R N Wakeling, C M Chittenden, J G Van der Waals, B Carpenter


Initial microbial colonisation of CCA-treated, creosote-treated and untreated Pinus sylvestris L. blocks in seawater
1996 - IRG/WP 96-10171
CCA-treated, creosote-treated and untreated Pinus sylvestris sample blocks were immersed in a seawater through-flow system for periods of between 1 and 265 hours. Samples were fixed immediately on removal and examined both qualitatively and quantitatively using the scanning electron microscope. This study of primary microbial colonisation showed differences between treatments in the diversity of m...
K Sturgess, A J Pitman


The resistance of wood coated with different solvent-borne paints against colonisation by decay fungi
2009 - IRG/WP 09-40468
This paper examines different solvent-borne paints characteristics and their decay resistance when applied on pine wood surface. It was determined by the standard ENV 839 procedure. The part of samples were subjected to accelerated ageing according to the EN 84 standard. The discussed commercial paint systems were typical stains or penetrating oil-based products, with or without biocides....
B Mazela, P Hochmańska


Étude in vitro de la colonisation et de la dégradation structurale du bois d'aubier de Pin sylvestre par la Mérule: Serpula lacrymans Schum. ex Fr. S. F. Gray
1979 - IRG/WP 198
The degradation of Scots pine sapwood cell walls by Serpula lacrymans, a brown rot fungus, is observed after various periods of exposure from two weeks to twelve weeks. The observation by microscopy shows that the hyphae of Serpula rapidly invade the wood tissues as cell wall degradation starts. That deterioration is not gradual, it is observed to be very irregular as well within the whole of the ...
D Dirol


L-joint trials: Part 2: The relationship between colonisation by decay fungi and long-term performance
2002 - IRG/WP 02-20251
Many of the L-joint trials conducted at BRE were assessed during the early stages to establish the rate of colonisation by fungi, principally basidiomycetes. At that time, a system of assessment incorporating colonisation studies was proposed for inclusion within the European Standard EN 330 but was rejected for the final version of the standard. The long-term trials associated with the colonisa...
J K Carey


L-joint trials: Part 1: Observations on the process of colonisation and decay
2002 - IRG/WP 02-20250
The first trials using the small L-joint, later adopted for use in BS EN 330 and AWPA Standard E9-97 were exposed at BRE in 1975 but all were assessed destructively during the early stages of exposure. The longest running trial which included replicates for long-term exposure will have been exposed for 20 years in July 2002. This paper provides an overview of the results of testing during that p...
J K Carey


Invasion and colonisation of bamboo culm material by stain and decay fungi
2002 - IRG/WP 02-10453
Two distinct stages of the fungal infection of bamboo culms can be identified: entry into the culm itself (invasion) and further colonisation by spread within the culm wall tissue. This laboratory study aimed to characterise different invasion strategies of a variety of fungi. Well-known isolates of white- (Coriolus versicolor, Schizophyllum commune), brown- (Coniophora puteana, Gloeophyllum tra...
G Kleist, I Morris, R J Murphy


The colonisation pattern of anaerobic fungi in wood
1989 - IRG/WP 1403
Timber foundations may be degraded in service in anaerobic conditions. The present work was conducted to investigate whether anaerobic fungi could colonise wood, using rumen fungi as test microorganisms. Strictly anaerobic fungi were isolated and purified from cow dung and goat and sheep rumen fluids respectively. Colonisation of Eucalyptus grandis and Pinus patula by these fungi and Neocallimasti...
A H Wiederhold, M Morrison, A A W Baecker


Differential susceptibility of living and dead timber to colonisation by sapstain and mould fungi
1998 - IRG/WP 98-10288
Field studies have revealed that when timber is irradiated (i.e. killed) it is more susceptible to colonisation by mould fungi than sapstain fungi. By comparison, freshly sawn timber shows very little mould colonisation, tending to be colonised by sapstain fungi. It appears, therefore that the physiological state of the wood may influence the pattern of colonisation. A laboratory trial was underta...
J R Williams, D J Dickinson, J F Webber


Effect of bioextracts on colonisation of radiata pine sapwood by three sapstain fungi
2003 - IRG/WP 03-10485
The objective of this study was to investigate the effect of aqueous bioextracts prepared following two-week incubation of forest soil, garden compost and chicken manure respectively, on the ability of Ophiostoma flocossum, Leptographium procerum and Sphaeropsis sapinea to colonise unseasoned radiata pine sapwood. The research showed that all bioextracts tested retarded the rate of fungal colonis...
J van der Waals, C Chittenden, B Kreber


Fungal colonisation of the keelson and associated structures of a nineteenth century wooden frigate: Concepts of community structure and development
1994 - IRG/WP 94-10072
The early stages of microbial colonization and succession of wooden surfaces exposed to the sea have been extensively studied as have the community structures of archaeological timbers subjected to submergence and then retrieval from the sea. The frigate UNICORN, a largely intact 19th century wooden ship based in Dundee, Scotland, provides the microbial ecologist with a unique opportunity to study...
N A White, J W Palfreyman, G M Smith


Colonisation and detection of New Zealand sapstain fungi
2000 - IRG/WP 00-10329
Sapstain is the bluish black discolouration in logs and timber caused in New Zealand by Sphaeropsis sapinea (Diploida pinea) and more than twenty different species of the Ophiostomataceae Family. Laboratory and field trials were conducted by inoculating radiata pine to establish staining colour and extent of colonisation with various New Zealand sapstaining fungal species. Colonisation patterns, c...
J M Thwaites, R L Farrell


The colonisation and succession of fungi in wood
1980 - IRG/WP 1107
A study of the microbial ecology of small stakes of Pinus sylvestris and Betula pendula, both untreated, and treated with a 1% solution of CCA is in progress. Results are presented for the early stages, up to 18 months.Two new techniques were developed linked by the philosophy of objective assessment. Isolations were made from the wood in a pre-determined pattern and transferred to four selective ...
C P Clubbe


Colonisation of painted wood by Aureobasidium pullulans - Analysis of features and consequences for failure in service
1996 - IRG/WP 96-10144
Wooden blocks of spruce were painted with different paint formulations. Water- and solvent-borne model and commercial paints were used. The painted wooden blocks were inoculated with a spore suspension of Aureobasidium pullulans (de Bary) Berkg. and placed in an environment of high humidity for 14 months. Different colonisation and growth patterns were observed on the different paint surfaces. The...
S L Bardage


Effects of the specimen position on fungal colonisation and wood decay by en 113 test fungi
1998 - IRG/WP 98-20136
For testing wood preservatives according to EN 113 it is common practice to plant the test blocks on neutral supports in order to prevent (1) a diffusion of chemicals into the agar medium and (2) an excessive moistening of the specimens. The procedure was employed in EN 350-1 for testing the natural durability of solid wood. It turned out to be of problematic nature because of the individual requ...
G Kleist, M-T Lenz, R-D Peek


The resistance of wood coated with different water-borne paints against colonisation by decay fungi
1996 - IRG/WP 96-10165
The susceptibility of wood painted with model paints of known composition to decay fungi was tested without previous weathering. Included in the study were five alkyd emulsion paints and five acrylic paints; one linseed oil paint and two solvent-borne alkyd paints. It was found that several components influenced the susceptibility of these paints. The results of the present study indicate that the...
J Bjurman


Comparing microbial colonisation and Decay Rates of Wood from Sound and Aphid-Killed Kenyan-Grown Mexican Cypress (Cupressus lusitanica)
2006 - IRG/WP 06-10599
Samples of wood from 10, 15, and 30-year old trees attacked and killed by the cypress aphid (Cinara cupressi) and sound Kenyan-grown Cupressus lusitanica trees were investigated for variations in moisture content, density and susceptibility to microbial decay. MC varied with tree age, a normal trend, and between samples from aphid-killed and sound trees. In samples from 10, 15 and 20-year old so...
R Venkatasamy


A step towards a better understanding of fungal colonization of modified wood - QRT-PCR studies
2008 - IRG/WP 08-10653
The area of wood protection is in a period of change. New wood protection systems have been developed while their mode of action remains insufficiently understood. The development of molecular methods provides potential tools to investigate the interaction between modified wood and decay fungi. One small step to tackle some of the unsolved questions about the mode of action of modified wood is tak...
G Alfredsen, A Pilgård, A Hietala


Durability and fungal colonisation patterns in wood samples after six years in soil contact evaluated with qPCR, microscopy, TGA, chitin- and ergosterol assays
2009 - IRG/WP 09-20402
There is a need to establish new objective and sensitive methods for early detection and quantification of decay fungi in wood materials. Molecular methods have proven to be a useful tool within wood protection issues, however, this field is still poorly explored and so far relatively few have used these methods within the field of wood deterioration. Among the techniques used in the indirect quan...
A Pilgård, G Alfredsen, I Børja, C Björdal


Early detection of colonisation by blue stain and mould on modified wood using PCR technique
2010 - IRG/WP 10-10730
The aim of this research was the early detection of fungal attack on the surfaces of modified and unmodified wood after natural weathering. The investigation was carried out by molecular techniques and the development and establishment of genus/species specific PCR for important moulds and sapstain fungi was part of this work. After adaption of the method, a monitoring of the succession of Ascomyc...
A Steitz, B Schmöllerl, N Pfabigan, S Bollmus, G Grüll


Estimation of wood degradation determined by visual inspection, mechanical testing and DNA characterisation – report after 3 years of exposure
2016 - IRG/WP 16-30700
The influence of two separate treatments, thermal modification and alkaline copper quaternary (ACQ, Silvanolin©) treatment, on fungal decay, visual disfigurement, deterioration in mechanical properties and fungal community structure was assessed on Norway spruce (Picea abies) and compared with the performance of two naturally more resistant tree species native to North America: Douglas fir (Pseud...
N Thaler, T Martinović, M Bajc, D Finžgar, H Kraigher, A Sinha, J J Morrell, M Humar