IRG Documents Database and Compendium


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A physiological role of the glyoxylate and TCA cycles in fruitbody formation of the coppertolerant brown-rot fungus Fomitopsis palustris
2002 - IRG/WP 02-10430
Changes in activity of the representative enzymes involved in biosynthesis of oxalic acid (oxalate) and carbon metabolism of glucose were investigated in relation to the fruit body formation of the copper-tolerant brown-rot fungus Fomitopsis palustris. Changes in specific activities of the two glyoxylate (GLOX) cycle key enzymes (isocitrate lyase (ICL) and malate synthase (MS)), the two oxalate-fo...
Jeong-Jun Yoon, T Hattori, M Shimada


A new concept of oxalic acid biosynthesis in physiology of copper-tolerant brown-rot fungi
2001 - IRG/WP 01-10394
Recently, a wide variety of roles of oxalic acid (oxalate) in wood decay systems have been receiving much attention. Copper tolerance of wood-rotting basidiomycetes has been believed to be due to the detoxification of copper wood preservatives by oxalate produced by these fungi. However, biochemical mechanism of oxalate biosynthesis in relation to physiology of wood-rotting fungi has not been eluc...
E Munir, T Hattori, M Shimada


Removal of heavy metals from treated wood using biological methods
2005 - IRG/WP 05-50226
Heavy metals were removed from wood treated with copper based preservatives using brown-rot fungus Fomitopsis palustris. The amount of effective elements removed by treatment methods was examined. The relationship between oxalic acid concentration and the amount of heavy metals removed from each treated wood was also investigated. The relationship between fungus weight and removal rate was also in...
Dong-won Son, Dong-heub Lee


A possible role of unique TCA cycles in wood-rotting basidiomycetes
2003 - IRG/WP 03-10461
The copper tolerant brown-rot fungus, Fomitopsis palustris, acquires metabolic energy by use of the constitutively-occurring Kornberg’s glyoxylate cycle coordinating with oxalate biosynthesis and glucose oxidation (Erman Munir et al. Proc. Natl. Acad. Sci. USA, (2001) 98, 11126-11130). Furthermore, this fungus does not have the normal TCA cycle, lacking 2-oxoglutarate dehydrogenase which is a ke...
E Munir, T Hattori, M Shimada


Chemically modified tannin and tannin-copper complexes as preservatives for wood
2001 - IRG/WP 01-30271
The efficacy of Mimosa tannin, chemically modified tannin, and tannin-copper complexes as wood preservatives was studied. When the tannin-ammonia-CuCl2 solutions were impregnated into wood specimens in a one-step procedure, a large quantity of the tannin-copper complex was fixed in the specimens. Little of the complex was leached from specimens by a weathering treatment, and these specimens showed...
H Yamaguchi


Sensitivity to Copper of Basidiospores from Copper Tolerant Fungi: Fomitopsis palustris and Oligoporus placentus
2010 - IRG/WP 10-10707
Copper continues to be an important fungicide in wood preservation. It is the primary component of the preservatives that have replaced chromated copper arsenate for treated wood in residential construction in North America. However, a co-biocide is normally needed to protect against copper tolerant organisms. Previous work has shown that the spores of at least one copper tolerant fungus, Oligopo...
C S Woo, P I Morris


Characterization of Pectinases from Brown-rot Fungus Fomitopsis palustris
2019 - IRG/WP 19-10951
Brown-rot fungi occur on softwood used as building materials and cause destructive breakdown of wood structure. Therefore, a more accurate understanding is important from the perspective of wood protection. Previous studies have reported that hyphae of brown-rot fungi go through bordered pits on tracheids when the fungi grow into softwood [Francis W.M.R.Schwarze (2007)], and torus existing in the ...
Y Tanaka, N Konno, T Suzuki, N Habu


Performance of Tebbacop in laboratory, fungus cellar and field tests
2000 - IRG/WP 00-30222
A novel organo-copper wood preservative ("Tebbacop") has been exposed to 12 years fungus cellar testing and 6 years above ground field testing. In the fungus cellar, Tebbacop at a retention of 0.053% Cu m/m oven dry wood out-performed CCA at a retention of 0.55% m/m oven dry wood. In above ground tests, L-joints treated to a Tebbacop retention of 0.012% Cu are performing as well as joints treated ...
M E Hedley, D R Page, B E Patterson


Recent soft-rot research in softwoods and hardwoods
1980 - IRG/WP 1108
The purpose of this paper is to describe briefly the current status of our research on soft-rot fungi. The work to be discussed is still in progress and any results described must be regarded as provisional....
J A Butcher


Cytochemical localization of hydrogen peroxide in brown rot fungus Tyromyces palustris by cerium chloride technique
1999 - IRG/WP 99-10299
Cerium chloride (CeCl3) was used to localize H2O2 cytochemically for studying relationship between ultrastructural and functional characteristics of cellulose degradation by brown rot fungi. This technique proved very useful in localizing discrete electron-densereactionproducts at high resolution with minimal nonspecific deposition. The cytochemical localization of extracellular H2O2 by CeCl3 usin...
Yoon Soo Kim, Seung-Gon Wi


Effectiveness and synergistic effects between copper and polymer betaine
1996 - IRG/WP 96-30097
Different formulations of "Copper Amine" and Polymer Betaine were studied. During laboratory tests a synergism between both active ingredients against soft rot and dry rot has been found. The efficacy against soft rot according to the "BAM method" and the European Standard ENV 807 depends only on the amount of copper. Long term tests in a fungus cellar for determining the relative protective effec...
H Härtner, V Barth


Lignin-copper, a new wood preservative without arsenic and chromium
1992 - IRG/WP 92-3702
A more environmentally sound treatment for wood with preservatives containing no arsenic or chromium, has been developed and studied on a laboratory scale. The method involves a first step impregnation with an aqueous solution containing modified, water-soluble kraft lignin followed by a second step involving impregnation with a copper salt solution to give fixation of the lignin into a water-inso...
B Ohlsson, R Simonson


Soil-bed studies. Part 2: The efficacy of wood preservative
1983 - IRG/WP 2205
Various methods of decay assessment were investigated. Three stages or phases of decay were identified which could be used to describe the efficacy of a preservative system or virulence of a soil-bed testing medium. These included the lag, decay, and senescent phase. Premature senescence could arise if wood samples became waterlogged. It was concluded that time to failure was unsuitable as a metho...
P Vinden, J F Levy, D J Dickinson


Fungus cellar testing as an evaluation method for performance of treated timber in ground contact
2001 - IRG/WP 01-20227
A fungus cellar method for the accelerated evaluation of performance of treated wood in ground contact is described. The test soil comprised of sandy loam, vermiculite and Japanese horticulture soil "Kanumatsuchi" in a ratio of 6:2:2 by volume. The soil was inoculated with the dominant test fungus isolated with selective medium from decayed wood samples. Pairs of treated and untreated wood specime...
Y Nagano


Assessment of the toxicity of some copper-, zinc- and boron-based wood preservatives to the cellar fungus Coniophora cerebella Schröet
1974 - IRG/WP 242
This article reports the use of a method based on the determination of the probability of the protection of timber against destruction by fungi. By converting the probability values to probit values and plotting them as a function of the amount of preservative retained in the timber, curves of the toxic effect are obtained, enabling any timber protection probability to be assessed....
V N Sozonova, D A Belenkov


Serpula lacrymans, The Dry Rot Fungus and its Tolerance towards Copper-based Wood Preservatives
2005 - IRG/WP 05-10555
Serpula lacrymans (Wulfen : Fries) Schröter, the dry rot fungus, is considered the most economically important wood decay fungus in temperate regions of the world i.e. northern Europe, Japan and Australia. Previously copper based wood preservatives were the most commonly used preservatives for pressure treatment of wood for building constructions. Because of a suspicion about tolerance toward cop...
A C Steenkjær Hastrup, F Green III, C A Clausen, B Jensen


Observations on the performance of copper-based wood preservatives in fungal cellar (soil-bed) tests
1994 - IRG/WP 94-20047
Fungal cellar (soil-bed) tests are considered to be an important tool for the evaluation of the performance of ground contact wood preservatives. Facilities of this type have been established world wide although caution has been exercised in their introduction into standard testing methods for the approval of wood preservatives. This is the result of concerns over the variability in the biological...
G R Williams, D Rudolph, M E Hedley, J A Drysdale, R F Fox


Decay and termite durabilities of heat-treated wood
2004 - IRG/WP 04-40272
Decay and termite resistances of Plato-treated timbers were evaluated using a modified JIS decay test method, feeding test in a laboratory scale and a field exposure against Reticulitermes speratus (Kolbe) or Coptotermes formosanus Shiraki. The results showed that no significant decay durability of the treated timbers although mass loss from decay slightly decreased in case of treated timbers. ...
S Doi, K Hanataa, E Kamonji, Yuuji Miyazaki


Preservative ability of wood to be fixed hydroxyl apatite substituted for antimicrobial metals
2001 - IRG/WP 01-30272
We succeeded in forming hydroxy apatite (HAp) in wood. HAp is non-toxicity and safe. Preservative ability of the wood, which substituted one part of Ca of constituent element of this HA p for antibacterial metals was measured. When Ca was substituted for Ag or Zn, mass loss in decay by brown-rot fungus F. palustris was restrained in about 50% (Ag) ~30% (Zn) of value of control specimen. However, w...
Y Haruhiko, I Sumaru


Tests with ammoniacal copper and alkyl ammonium compounds as wood preservatives
1984 - IRG/WP 3299
Formulations based on copper and alkyl ammonium compounds in ammonia solution have been tested in a fungus cellar on Pinus radiata and Fagus sylvatica. This type of products gives promising results as wood preservatives, especially on hardwood and are safe to destroy by e.g. combustion. The best results were achieved with a dialkyl ammonium compound, Cu/octyldecyl dimethyl ammonium chloride (NH3)....
C-E Sundman


Variation of natural durability of sugi (Cryptomeria japonica) wood in 15 clones examined by decay test (Preliminary report)
2004 - IRG/WP 04-10526
Natural durability of wood in 27 trees from 15 clones of sugi (Cryptomeria japonica)  was investigated by an accelerated decay test. Thirty years old trees were collected from a clonal trial in Ibaraki prefecture, Japan. Natural durability is usually evaluated in heartwood. Mass losses of heartwood caused by a brown rot decay fungus, Fomitopsis palustris and a white rot decay fungus, Py...
K Yamamoto, A Tamura, R Nakada


Targeted inhibition of wood decay (Using everything but the kitchen sink)
1997 - IRG/WP 97-10203
Low molecular weight oxidative decay agents have been implicated in the degradation of wood by brown-rot decay as evidenced by chemical analysis of brown-rotted wood and detection of oxalic acid and hydroxy radicals. Fenton chemistry (H2O2 / Fe++) is often proposed as the mechanism for generating hydroxy radicals. Previous authors have shown iron to enhance the brown-rot hydrolysis of wood, while ...
F Green III, T A Kuster, T L Highley


Effects of cyproconazole and copper sulphate on the length of the hyphal growth unit (HGU) of the white-rot fungus Coriolus versicolor
2003 - IRG/WP 03-10473
Wood decay basidiomycetes have been shown to produce appreciable quantities of extracellular mucilaginous materials (ECMM). The relationship between ECMM and total biomass production has been investigated in the white-rot fungus Coriolus versicolor (CTB 863 A). Differences in the amount of ECMM produced by the fungus proportionally to the total biomass, were observed under a range of physiological...
D Vesentini, D J Dickinson, R J Murphy


Growth of the copper tolerant brown rot fungus Antrodia vaillantii on different substrates
1995 - IRG/WP 95-10121
In recent years the copper tolerant brown rot fungus Antrodia vaillantii caused severe damages on impregnated wood in ground contact. The pattern of decay gave the impression that impregnated wood was even more severely attacked than unimpregnated. To investigate this question more closely laboratory tests were carried out. In a "choice test" Antrodia vaillantii grew preferably towards CC-impregna...
H Leithoff, I Stephan, M-T Lenz, R-D Peek


Silicic acid-Boric acid complexes as wood preservatives
2001 - IRG/WP 01-30273
A silicic acid monomer aqueous solution (SAMS) or colloidal silicic acid solution (CSAS) was combined with various metal compounds or boric acid. Agents where SAMS or CSAS was combined with boric acid gave good protection against decay caused by the brown-rot fungus Fomitopsis palustris, the treated wood (Cryptomera japonica D. Don) specimens after the leaching test maintained a high resistance to...
H Yamaguchi


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