Your search resulted in 706 documents. Displaying 25 entries per page.
Analysis of volatile emissions as an aid in the diagnosis of dry rot
1992 - IRG/WP 92-2393
The dry rot fungus, Serpula lacrymans was grown in pure culture on malt extract and on sapwood of pine. The volatile compounds emitted from the cultures were determined by diffusion sampling on tubes filled with Tenax TA, thermal desorption and gas-chromatography-mass-spectrometry in order to find markers for attack of the fungus....
J Bjurman, J Kristensson
Effect of mineral wools on growth and decay capacities of Serpula lacrymans and some other brown-rot fungi
1991 - IRG/WP 1481
The influence of stone wool and glass wool on growth and decay capacities of Serpula lacrymans and some other brown-rot fungi was studied. Mass losses of pine wood caused by Serpula lacrymans and Gloeophyllum trabeum were increased when stone wool was present. Glass wool had no influence on mass losses. Coniophora puteana, Serpula lacrymans and Gloeophyllum trabeum grew into stone wool and destroy...
L Paajanen, A-C Ritschkoff
Wood degradation mechanisms by the brown rot fungus Gloeophyllum trabeum
1997 - IRG/WP 97-10229
A mechanism for the degradation of wood by the brown rot fungus Gloeophyllum trabeum is outlined. The mechanism includes the function of redox-cycling, low molecular weight phenolic derivatives which sequester and reduce iron in acidic environments. The role of oxalate for the sequestration of iron (hydr)oxides and the pH dependent transfer of iron to the G. trabeum phenolic chelators, as well as ...
B Goodell, J Jellison
Iron in stone wool - one reason for the increased growth and decay capacity of Serpula lacrymans
1992 - IRG/WP 92-1537
The chemical compositions of stone wool and glass wool were analysed. There was more iron in the stone wool than in the glass wool. It was found that iron present in stone wool was easily dissolved by oxalic acid that Serpula lacrymans is able to produce. The stone wool promoted the decay of pine wood by Serpula lacrymans. The glass wool had no effect on the decay capacity of Serpula Iacrymans. Th...
L Paajanen, A-C Ritschkoff
Investigation of microwave as a means of eradicating dry rot attack in buildings
1992 - IRG/WP 92-1545
A microwave-apparatus developed for treatment of building-structures infested by dry rot was tested in the laboratory. The test-conditions simulated treatment of either infested timber or infested brickwork. Sawdust samples, both wet and dry, infested by viable mycelium of Serpula lacrymans were packed in glass-tubes. The tubes were then placed in the middle of either 20 cm thick wooden beams or 3...
C Kjerulf-Jensen, A P Koch
Identification of terminal structures in cellulose degraded by the brown-rot fungus Postia placenta
1989 - IRG/WP 1389
To gain insight into the biochemical mechanism employed by brown-rot fungi to depolymerize cellulose, we identified the end-groups of chemically pure cellulose that had been depolymerized by the brown-rot fungus, Postia placenta. The depolymerized cellulose was acid hydrolyzed and the anion fractions isolated by ion chromatography. Sugar acids were identified by gas chromatographic and mass spectr...
T K Kirk, T L Highley, R E Ibach, M D Mozuch
Biosynthesis of ß-Glucan microfibrils by cellular fractions from brown-rot fungus Postia placenta (MAD-698 and ME-20) and white-rot fungus Schizophyllum commune (MAD-619)
1993 - IRG/WP 93-10025
In this study, we compared the brown rot fungus Postia placenta (MAD-698 and ME-20) with the white rot fungus Schizophyllum commune (MAD-619) to determine the location and distribution of glucan synthetase. We also measured the soluble protein content in subcellular fractions obtained by differential centrifugation MAD-698 is a degradative isolate, but ME-20 and MAD-619 do not produce significant ...
S C Croan, T L Highley
Fungus cellar testing as an evaluation method for performance of treated timber in ground contact
2001 - IRG/WP 01-20227
A fungus cellar method for the accelerated evaluation of performance of treated wood in ground contact is described. The test soil comprised of sandy loam, vermiculite and Japanese horticulture soil "Kanumatsuchi" in a ratio of 6:2:2 by volume. The soil was inoculated with the dominant test fungus isolated with selective medium from decayed wood samples. Pairs of treated and untreated wood specime...
Y Nagano
An electron spin resonance study of manganese changes in wood decayed by the brown-rot fungus, Postia placenta
1988 - IRG/WP 1359
Electron spin resonance (ESR) spectrometry was used to examine wood decay by the brown-rot fungus, Postia placenta. Wood slivers of Douglas-fir, white fir, redwood, sweetgum and yellow poplar were incubated for 4 weeks in custom-made quartz ESR tubes with or without Postia placenta. In all wood species without fungus, a weak partially resolved signal (about g=2, presumably due to manganese) was de...
B Illman, D C Meinholtz, T L Highley
Occurrence, prevention and repair of Dry Rot
1990 - IRG/WP 1439
Information about the frequency, detection, identification and repair of Dry Rot attacks (Serpula lacrymans) is summarized from 13 European countries. Based on 28 completed and returned questionnaires it appears that Dry Rot attacks are recognized in all countries participating, and that there is an appreciable similarity in the frequency of attacks and methods of detection and identification. How...
A P Koch
Evidence for wood cell wall degradation by the blue stain fungus Botryodiplodia theobromae Pat
1994 - IRG/WP 94-10077
Botryodiplodia theobromae Pat., a world wide ubiquitous polyfagus sapstain fungus, was found able to destroy the cell walls of birch fibres (Betula verrucosa Ehrh.) but not Caribbean (Pinus caribaea var. hondurensis Barr. and Golf.) and Scots pine (Pinus sylvestris L.) tracheids. The fungus caused characteristic erosion of fibre cell walls similar to soft rot type 2; destruction of the S1 - S2 int...
O Encinas, G F Daniel
The growth and behaviour of Australian isolates of dry rot fungi, mainly Serpula lacrymans (Schum. ex Fr.) S.F. Gray. A comparision with the world literature
1983 - IRG/WP 1190
In Australia Serpula lacrymans occupies a rather unique environment, being restricted to growth within basal regions of buildings containing masonry. Its rate of timber attack in these situations appears to be far faster than that experienced in other parts of the world. Therefore the question arises as to how Australian isolates (particularly 16508 which has become a "standard" test fungus for so...
J D Thornton, O Collett
The critical moisture and temperature conditions for the growth of some mould fungi and the brown rot fungus Coniophora puteana on wood
1988 - IRG/WP 1369
The growth of some mould fungi on pine and spruce sapwood was studied in 40 different constant moisture and temperature conditions. The temperature range was between +10 and +40°C and the relative humidities varied from 76 to 100% relative humidity (RH). The incubation time was 12 weeks. The mixed mould inoculation used included typical fungi growing on wood: Aspergillus versicolor, Cladosporium ...
H Viitanen, L Paajanen
Generation of hydroxyl radical by the brown-rot fungus, Postia placenta
1988 - IRG/WP 1360
In an electron spin resonance (ESR) survey of various liquid cultures and wood slivers innoculated with the brown-rot fungus, Postia placenta, the spin trap dimethyl-l-pyrroline N-oxide (DMPO) was used to detect the presence of the hydroxyl radical. The ESR spectra for the paramagnetic DMPO- hydroxyl radical adduct was observed in (1) nitrogen-limited, liquid cultures having 1.0% glucose or 0.1% c...
B Illman, D C Meinholtz, T L Highley
Observations on the performance of copper-based wood preservatives in fungal cellar (soil-bed) tests
1994 - IRG/WP 94-20047
Fungal cellar (soil-bed) tests are considered to be an important tool for the evaluation of the performance of ground contact wood preservatives. Facilities of this type have been established world wide although caution has been exercised in their introduction into standard testing methods for the approval of wood preservatives. This is the result of concerns over the variability in the biological...
G R Williams, D Rudolph, M E Hedley, J A Drysdale, R F Fox
Preservative effect of cellulose insulation material against some mould fungi and brown rot fungus Coniophora puteana in pine sapwood
1991 - IRG/WP 1484
The influence of mineral wool and loose-fill cellulose wool on biodeterioration of pine sapwood was studied. The test fungi were mould fungi Aspergillus versicolor, Cladosporium sphaerospermum, Penicillium sp. and Aureobasidium pullullans and brown rot fungus Coniophora puteana. Cellulose wool inhibited the growth of mould fungi on the face of wood contacted with insulation material at RH of 97 an...
H Viitanen
Oxalic acid quantification, oxaloacetase assay and ESI localization of P, C, and Fe from the brown rot fungus Postia placenta
1994 - IRG/WP 94-10063
The mechanism by which brown-rot fungi initiate depolymerization of holocellulose in wood remains unknown. Recently, oxalic acid (OA) has received considerable attention in cellulose breakdown by brown-rot fungi. The OA could serve as a proton donor for hydrolytic or an electron donor for oxidative (Fenton's reaction-H2O2/Fe2+) cleavages of cellulose. The acid may originate via oxaloaceta...
C R Jordan, W V Dashek, T L Highley
Proposed model for the penetration and decay of wood by the hyphal sheath of the brown-rot fungus Postia placenta
1989 - IRG/WP 1391
Scanning electron microscopy (SEM) of Pinus sp. decayed by the brown-rot fungus Postia placenta confirmed the existence of extracellular membranous structures previously described by transmission electron microscopy (TEM). These structures appear to be an integral part of the hyphal sheath and assume a variety of forms including lamellar sheets, fibrils, and vesicles. These structures were observe...
F Green III, M J Larsen, L L Murmanis, T L Highley
Production of exraxellular hydrogen peroxide and oxalic acid by brown-rot fungus Poria placenta
1995 - IRG/WP 95-10112
Hydrogen peroxide and oxalic acid have been suggested to be essential in the degradation of wood carbohydrates by brown-rot fungi. Hydrogen peroxide has been suggested to be one of the diffusible low molecular weight agents produced by brown-rot fungi for the degradation of wood carbohydrates by generating highly active radicals. The production of hydrogen peroxide and oxalic acid by Poria placent...
A-C Ritschkoff, M Rättö, J Buchert, L Viikari
Detection of a brown-rot fungus using serological assays
1986 - IRG/WP 1305
Polyclonal antisera produced to Poria placenta (Fr.) Cooke were used in two tests to qualitatively assay for the fungus. Fungal hyphae were fixed to slides and fluorescent antibody (FA) techniques used to visualize the hyphae under the microscope. Fluorescence of non-Poria fungi, when present, could be reduced but not eliminated by cross-absorbing the sera with these fungi. The antisera was also u...
B Goodell, J Jellison
A laboratory technique to measure the performance of preservative treated hardwoods in ground contact
1982 - IRG/WP 2172
A laboratory technique to measure the performance of preservative treated timber in ground contact is described. It uses partially sealed blocks of timber which are treated, leached and sawn into small stakelets for soil exposure in a fungal cellar. Performance is monitored by the loss of static bending strength with time, and a simple apparatus for measuring the deflection of a stakelet under a l...
E F Baines
Secretion of ligninolytic enzymes by hyphal autolysis of the white rot fungus Phanerochaete chrysosporium
1991 - IRG/WP 1480
The secretion of ligninases by Phanerochaete chrysosporium was investigated with polyclonal antibodies, followed by immmunogold-silver staining and light microscopy. After growing fungal mycelium on nitrocellulose, extracellular ligninases were detected around old, plasmaless hyphae, but not at arthrospores, chlamydospores, or blastoconidia. Labeling of fungal hyphae on coverslip cultures was obse...
R Lackner, E Srebotnik, K Messner
Immuno-electron microscopic localization of extracellular metabolites in spruce wood decayed by brown-rot fungus Postia placenta
1990 - IRG/WP 1441
Degradation by Postia placenta in spruce and birch wood was shown to occur not only in the wood cell wall but also in the middle lamellae region. Middle lamellae was often found to be degraded along the centerline so that cells could separate along this line. Extracellular membrane structures were found surrounding the hyphae and this matrix labelled positively with antisera produced to Postia pla...
Y S Kim, B Goodell, J Jellison
Respiration measurement of dry-rot
1995 - IRG/WP 95-10095
Methods for measuring the production of carbondioxide from wood infected with the dry-rot fungus were evaluated. By enclosing whole wood blocks and measuring the concentration of gas in the headspace by gas chromatography, an exponential increase in the concentration of CO2 was observed for at least 2 months. This technique could therefore present a method for evaluating various treatments of dry-...
L Toft
On the biotope of dry rot (Serpula lacrymans) in the wild
1995 - IRG/WP 95-10123
The True Dry rot fungus was found in the Himalayas several times in this century. The finds were able to cross breed with Serpula lacrymans from Denmark and therefore all belong to this species (Harmsen 1960). In order to understand its successful colonisation in buildings we were interested in studying the natural biotope, i.e. the moisture, temperature and soil relationships. All together 15 fru...
J Bech-Andersen, S A Elborne, K Bech-Andersen